Encyclopedia of Signaling Molecules

2018 Edition
| Editors: Sangdun Choi


  • Ales TichyEmail author
  • Jaroslav Pejchal
Reference work entry
DOI: https://doi.org/10.1007/978-3-319-67199-4_101620


Historical Background

DNA-dependent protein kinase (DNA-PK) was discovered in early 1990s, when the catalytic activity of a protein kinase triggered by double-stranded DNA was reported for the first time. Interestingly, it was noted that the kinase functions efficiently only in the presence of linear but not supercoiled DNA (Carter et al. 1990). Independently, DNA-PK was reported to phosphorylate transcription factor Sp1 and a number of other DNA-binding proteins. Furthermore, protein Ku was identified as an essential component of DNA-PK and confirmed as a binding subunit that served to recruit DNA-PK catalytic subunit (DNA-PKcs) (Gottlieb and Jackson 1993). Another important breakthrough came from Stamato and his colleagues in 1994, who reported lack of double-strand break (DSB) DNA binding activity in radiosensitive rodent cell line and attributed it to the Ku heterodimer absence (Getts and Stamato 1994). Since then,...

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© Springer International Publishing AG 2018

Authors and Affiliations

  1. 1.Faculty of Military Health Sciences, Department of RadiobiologyUniversity of DefenceHradec KrálovéCzech Republic