Abstract
Production of secondary metabolites from in vitro cell and hairy root cultures (CHRC) of medicinal and aromatic plants (MAP) is considered a promising alternative to gathering plant material from MAP natural populations, often a reason for their overexploitation and even extinction. However, most of the valuable secondary metabolites extracted from different MAP species are present in very low amounts in the respective CHRC. Plant metabolic engineering offers an attractive opportunity to increase the content of target secondary metabolites in engineered transgenic CHRC for production at feasible levels. Moreover, applying metabolic engineering makes it possible to redirect target metabolic pathway(s) in the transgenic CHRC to produce new compounds not present in the wild plant itself. This chapter describes the strategies and experimental toolbox for plant metabolic engineering with examples from engineering secondary metabolite production in CHRC from MAP, as well as a review of these century reported studies on metabolic engineering of CHRC. The directions and prospects for CHRC metabolic engineering applications in production of valuable secondary metabolites are discussed
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Abbreviations
- CHRC:
-
Cell and hairy root culture
- CS:
-
Cell suspension
- HR:
-
Hairy roots
- MAP:
-
Medicinal and aromatic plants
- ME:
-
Metabolic engineering
- RNAi:
-
RNA interference
- TF:
-
Transcription factor
- TIA:
-
Monoterpenoid indole alkaloids
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Rusanov, K., Atanassov, A., Atanassov, I. (2018). Engineering Cell and Organ Cultures from Medicinal and Aromatic Plants Toward Commercial Production of Bioactive Metabolites. In: Pavlov, A., Bley, T. (eds) Bioprocessing of Plant In Vitro Systems. Reference Series in Phytochemistry. Springer, Cham. https://doi.org/10.1007/978-3-319-54600-1_8
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