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Table 2 Example of representative recombinant proteins produced via in vitro cultures

From: Platforms for Plant-Based Protein Production

Recombinant proteins

Host plant species

Promoter

Protein yieldsa

References

Plant suspension cells

Anti-HIV antibody 2G12

N. tabacum cv BY-2

CaMV35S

12 mg L−1

[52]

Human antibody M12

N. tabacum cv BY-2

CaMV35S

107 mg L−1

[53]

Anti-hepatitis B surface antigen (HBsAg) mAb

N. tabacum cv BY-2

CaMV35S

15 mg L−1

[54]

Human growth hormone (hGH)

N. tabacum cv BY-2

CaMV35S

35 mg L−1

[49]

O. sativa cv Donjin

RAmy3D

[55]

Human interferon α2b (hIFNα2)

N. tabacum cv BY-2

CaMV35S

28 mg L−1

[48]

Human α-1-antitrypsin (rAAT)

O. sativa

RAmy3D

247 mg L−1

[33]

D. carota

CaMV35S

www.protalix.com

hGM-CSF

O. sativa

RAmy3D

129 mg L−1

[56]

Glucocerebrosidase

D. carota

CaMV35S

N/A

[57]

α-Galactosidase-A (PRX-102)

D. carota

CaMV35S

N/A

[58]

www.protalix.com

Human interleukin-12 (IL-12)

O. sativa

RAmy3D

31 mg L−1

[59]

Human serum albumin (hSA)

O. sativa

RAmy3D

45 mg L−1

[60]

Enhanced GFP

N. tabacum cv BY-2

CaMV35S

125–131 mg L−1

[61]

Hairy roots

Human antibody M12

N. tabacum

CaMV35S

5.9 mg L−1

[62]

Human antibody H10

N. tabacum

CaMV35S

2–3 mg L−1

[63]

N. benthamiana (DeltaXTFT)

Hepatitis B surface antigen (HBsAg)

Solanum tuberosum (var. Kufri bahar )

(Aocs) 3 AmasPmas a

97.1 ng g−1FW

[64]

14D9 murine IgG1

N. tabacum

CaMV35S

64 mg L−1

[65]

Human epidermal growth factor (hEGF)

N. tabacum

CaMV35S

2 ug g−1FW

[66]

Thaumatin

N. tabacum

CaMV35S

2.6 mg L−1

[67]

GFP

Brassica rapa rapa (turnip)

CaMV35S

120 mg L−1

[68]

Human tissue plasminogen activator (tPA)

Cucumis melo L cv Geumssaragi-euncheon or N. tabacum

CaMV35S

798 ug g−1FW or 900 ng mg TSP

[69, 70]

Moss

Vascular endothelial growth factor (VEGF)

Physcomitrella patens

CaMV35S

656 μg g−1DW

[71, 72]

Erythropoietin (EPO)

P. patens

PpUbq1 b

250 μg g−1DW

[73, 74]

Complement factor H

P. patens

Ppact5-P c

23.2 μg g−1DW (secreted); 25.8 μg g−1DW (intracellular)

[75]

Multi-epitope fusion protein (HIV vaccine)

P. patens

Pca200P d

3.7 μg g−1FW

[76]

α-Galactosidase, β-glucocerebrosidase, FGF7/KGF

P. patens

N/A

N/A

[77]; www.greenovation.com

  1. aFW fresh weight, DW dry weight, TSP total soluble protein, NA date not available
  2. bChimeric super-promoter consisting of three copies of the octopine synthase activator (Aocs) and one copy of the mannopine synthase activator (Amas) located upstream of the mannopine synthase promoter (Pmas)
  3. cPpact5-P: P. patens actin5 gene promoter
  4. dPpUbq1: Physcomitrella ubiquitin gene-derived promoter
  5. ePca200P: P. patens carbonic anhydrase 200 promoter
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