Abstract
Regeneration of damaged or malfunctioning tissues or organs is important goal of tissue engineering. Various techniques such as cell sheet engineering, cell spheroids, scaffold assisted methods and 3D printing of the cells with polymers have been tested in tissue engineering. Among these techniques, scaffold assisted method is extensively employed as it acts as a supporting matrix for the cells, providing suitable microenvironment to facilitate the cell attachment, proliferation and differentiation. In this context, designing scaffolds which mimics extracellular matrix (ECM) is essential to regenerate the damaged tissues and organs. The electrospinning technique is a versatile tool to fabricate ECM mimicking scaffolds. ECMs obtained using this technique are highly desired due to their excellent physical properties such as high surface area. High surface area assists in immobilizing bulk quantity of biomolecules like growth factors, enzymes, and drugs which provide favorable microenvironment to cells. Hence, the electrospinning is a suitable tool in regenerative tissue engineering. This chapter discusses about the importance of electrospun polymer fibers for regeneration of various tissues including bone, cartilage, heart muscles, liver and neural tissues. Influence of properties such as surface chemistry, mechanical properties and porosity on gene expression of stem cell will be addressed. The impact of biomolecule immobilization, electrospun fiber size, fiber orientation and fiber morphology on stem cell differentiation is also discussed. The performance of biopolymer and synthetic degradable polymer based electrospun fibers in tissue engineering will also be briefly reported.
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References
Cheng L, Sun X, Zhao X, Wang L, Yu J, Pan G et al (2016) Surface biofunctional drug-loaded electrospun fibrous scaffolds for comprehensive repairing hypertrophic scars. Biomaterials 83:169–181
Yamato M, Okano T (2004) Cell sheet engineering. Mater Today 7:42–47
Drury JL, Mooney DJ (2003) Hydrogels for tissue engineering: scaffold design variables and applications. Biomaterials 24:4337–4351
Hutmacher DW (2000) Scaffolds in tissue engineering bone and cartilage. Biomaterials 21:2529–2543
Han D, Gouma P-I (2006) Electrospun bioscaffolds that mimic the topology of extracellular matrix. Nanomedicine: Nanotechnol Biol Med 2:37–41
Huang Z-M, Zhang YZ, Kotaki M, Ramakrishna S (2003) A review on polymer nanofibers by electrospinning and their applications in nanocomposites. Compos Sci Technol 63:2223–2253
Kidoaki S, Kwon IK, Matsuda T (2005) Mesoscopic spatial designs of nano- and microfiber meshes for tissue-engineering matrix and scaffold based on newly devised multilayering and mixing electrospinning techniques. Biomaterials 26:37–46
Tong H-W, Wang M (2007) Electrospinning of aligned biodegradable polymer fibers and composite fibers for tissue engineering applications. J Nanosci Nanotechnol 7:3834–3840
Wang X, Ding B, Li B (2013) Biomimetic electrospun nanofibrous structures for tissue engineering. Mater Today 16:229–241
Li W-J, Mauck RL, Cooper JA, Yuan X, Tuan RS (2007) Engineering controllable anisotropy in electrospun biodegradable nanofibrous scaffolds for musculoskeletal tissue engineering. J Biomech 40:1686–1693
Haider A, Haider S, Kang I-K (2015) A comprehensive review summarizing the effect of electrospinning parameters and potential applications of nanofibers in biomedical and biotechnology. Arab J Chem
Agarwal S, Wendorff JH, Greiner A (2008) Use of electrospinning technique for biomedical applications. Polymer 49:5603–5621
Vieira MGA, da Silva MA, dos Santos LO, Beppu MM (2011) Natural-based plasticizers and biopolymer films: a review. Eur Polym J 47:254–263
Okamoto M, John B (2013) Synthetic biopolymer nanocomposites for tissue engineering scaffolds. Prog Polym Sci 38:1487–1503
Nagarajan S, Pochat-Bohatier C, Teyssier C, Balme S, Miele P, Kalkura N et al (2016) Design of graphene oxide/gelatin electrospun nanocomposite fibers for tissue engineering applications. RSC Adv 6:109150–109156
Kim SJ, Yang DH, Chun HJ, Chae GT, Jang JW, Shim YB (2013) Evaluations of chitosan/poly(D,L-lactic-co-glycolic acid) composite fibrous scaffold for tissue engineering applications. Macromol Res 21:931–939
West JL, Hubbell JA (1999) Polymeric biomaterials with degradation sites for proteases involved in cell migration. Macromolecules 32:241–244
Malliaras K, Kreke M, Marbán E (2011) The stuttering progress of cell therapy for heart disease. Clin Pharmacol Ther 90:532–541
Sepantafar M, Maheronnaghsh R, Mohammadi H, Rajabi-Zeleti S, Annabi N, Aghdami N et al (2016) Stem cells and injectable hydrogels: synergistic therapeutics in myocardial repair. Biotechnol Adv 34:362–379
Bhowmick S, Scharnweber D, Koul V (2016) Co-cultivation of keratinocyte-human mesenchymal stem cell (hMSC) on sericin loaded electrospun nanofibrous composite scaffold (cationic gelatin/hyaluronan/chondroitin sulfate) stimulates epithelial differentiation in hMSCs: in vitro study. Biomaterials 88:83–96
Kai D, Wang Q-L, Wang H-J, Prabhakaran MP, Zhang Y, Tan Y-Z et al (2014) Stem cell-loaded nanofibrous patch promotes the regeneration of infarcted myocardium with functional improvement in rat model. Acta Biomater 10:2727–2738
Teitelbaum SL (2000) Bone resorption by osteoclasts. Science 289:1504–1508
Gómez-Guillén MC, Giménez B, López-Caballero ME, Montero MP (2011) Functional and bioactive properties of collagen and gelatin from alternative sources: a review. Food Hydrocoll 25:1813–1827
Asghar A, Henrickson RL (1982) Chemical, biochemical, functional, and nutritional characteristics of collagen in food systems. Adv Food Res 28:231–372
Narayanan N, Jiang C, Uzunalli G, Thankappan SK, Laurencin CT, Deng M (2016) Polymeric electrospinning for musculoskeletal regenerative engineering. Regen Eng Transl Med 2:69–84
Matthews JA, Wnek GE, Simpson DG, Bowlin GL (2002) Electrospinning of collagen nanofibers. Biomacromolecules 3:232–238
Shih Y-RV, Chen C-N, Tsai S-W, Wang YJ, Lee OK (2006) Growth of mesenchymal stem cells on electrospun type I collagen nanofibers. Stem Cells 24:2391–2397
Dhand C, Ong ST, Dwivedi N, Diaz SM, Venugopal JR, Navaneethan B et al (2016) Bio-inspired in situ crosslinking and mineralization of electrospun collagen scaffolds for bone tissue engineering. Biomaterials 104:323–338
Song J-H, Kim H-E, Kim H-W (2008) Electrospun fibrous web of collagen–apatite precipitated nanocomposite for bone regeneration. J Mater Sci Mater Med 19:2925–2932
Su Y, Su Q, Liu W, Lim M, Venugopal JR, Mo X et al (2012) Controlled release of bone morphogenetic protein 2 and dexamethasone loaded in core–shell PLLACL–collagen fibers for use in bone tissue engineering. Acta Biomater 8:763–771
Wang K, Chen X, Pan Y, Cui Y, Zhou X, Kong D et al (2015) Enhanced vascularization in hybrid PCL/gelatin fibrous scaffolds with sustained release of VEGF. Biomed Res Int 2015:10
Zhiwei R, Shiqing M, Le J, Zihao L, Deping L, Xu Z et al (2017) Repairing a bone defect with a three-dimensional cellular construct composed of a multi-layered cell sheet on electrospun mesh. Biofabrication 9:025036
Zhang Y, Ouyang H, Lim CT, Ramakrishna S, Huang Z-M (2005) Electrospinning of gelatin fibers and gelatin/PCL composite fibrous scaffolds. J Biomed Mater Res B Appl Biomater 72B:156–165
Kwak S, Haider A, Gupta KC, Kim S, Kang I-K (2016) Micro/nano multilayered scaffolds of PLGA and collagen by alternately electrospinning for bone tissue engineering. Nanoscale Res Lett 11:323
Kim K-H, Jeong L, Park H-N, Shin S-Y, Park W-H, Lee S-C et al (2005) Biological efficacy of silk fibroin nanofiber membranes for guided bone regeneration. J Biotechnol 120:327–339
Shao W, He J, Sang F, Ding B, Chen L, Cui S et al (2016) Coaxial electrospun aligned tussah silk fibroin nanostructured fiber scaffolds embedded with hydroxyapatite–tussah silk fibroin nanoparticles for bone tissue engineering. Mater Sci Eng C 58:342–351
Niu B, Li B, Gu Y, Shen X, Liu Y, Chen L (2017) In vitro evaluation of electrospun silk fibroin/nano-hydroxyapatite/BMP-2 scaffolds for bone regeneration. J Biomater Sci Polym Ed 28:257–270
Li C, Vepari C, Jin H-J, Kim HJ, Kaplan DL (2006) Electrospun silk-BMP-2 scaffolds for bone tissue engineering. Biomaterials 27:3115–3124
Chen J-P, Chen S-H, Lai G-J (2012) Preparation and characterization of biomimetic silk fibroin/chitosan composite nanofibers by electrospinning for osteoblasts culture. Nanoscale Res Lett 7:170
Homayoni H, Ravandi SAH, Valizadeh M (2009) Electrospinning of chitosan nanofibers: processing optimization. Carbohydr Polym 77:656–661
Geng X, Kwon O-H, Jang J (2005) Electrospinning of chitosan dissolved in concentrated acetic acid solution. Biomaterials 26:5427–5432
Min B-M, Lee SW, Lim JN, You Y, Lee TS, Kang PH et al (2004) Chitin and chitosan nanofibers: electrospinning of chitin and deacetylation of chitin nanofibers. Polymer 45:7137–7142
Jayakumar R, Prabaharan M, Nair SV, Tamura H (2010) Novel chitin and chitosan nanofibers in biomedical applications. Biotechnol Adv 28:142–150
Sangsanoh P, Suwantong O, Neamnark A, Cheepsunthorn P, Pavasant P, Supaphol P (2010) In vitro biocompatibility of electrospun and solvent-cast chitosan substrata towards Schwann, osteoblast, keratinocyte and fibroblast cells. Eur Polym J 46:428–440
Jayakumar R, Menon D, Manzoor K, Nair SV, Tamura H (2010) Biomedical applications of chitin and chitosan based nanomaterials—a short review. Carbohydr Polym 82:227–232
Croisier F, Jérôme C (2013) Chitosan-based biomaterials for tissue engineering. Eur Polym J 49:780–792
Khajavi R, Abbasipour M, Bahador A (2016) Electrospun biodegradable nanofibers scaffolds for bone tissue engineering. J Appl Polym Sci 133:n/a–n/a
Amaral IF, Lamghari M, Sousa SR, Sampaio P, Barbosa MA (2005) Rat bone marrow stromal cell osteogenic differentiation and fibronectin adsorption on chitosan membranes: the effect of the degree of acetylation. J Biomed Mater Res A 75A:387–397
Zhang Y, Venugopal JR, El-Turki A, Ramakrishna S, Su B, Lim CT (2008) Electrospun biomimetic nanocomposite nanofibers of hydroxyapatite/chitosan for bone tissue engineering. Biomaterials 29:4314–4322
Yilgor P, Tuzlakoglu K, Reis RL, Hasirci N, Hasirci V (2009) Incorporation of a sequential BMP-2/BMP-7 delivery system into chitosan-based scaffolds for bone tissue engineering. Biomaterials 30:3551–3559
Filion TM, Kutikov A, Song J (2011) Chemically modified cellulose fibrous meshes for use as tissue engineering scaffolds. Bioorg Med Chem Lett 21:5067–5070
Romero R, Chubb L, Travers JK, Gonzales TR, Ehrhart NP, Kipper MJ (2015) Coating cortical bone allografts with periosteum-mimetic scaffolds made of chitosan, trimethyl chitosan, and heparin. Carbohydr Polym 122:144–151
Ghasemi-Mobarakeh L, Prabhakaran MP, Morshed M, Nasr-Esfahani M-H, Ramakrishna S (2008) Electrospun poly(ɛ-caprolactone)/gelatin nanofibrous scaffolds for nerve tissue engineering. Biomaterials 29:4532–4539
Li W-J, Tuli R, Huang X, Laquerriere P, Tuan RS (2005) Multilineage differentiation of human mesenchymal stem cells in a three-dimensional nanofibrous scaffold. Biomaterials 26:5158–5166
Yoshimoto H, Shin YM, Terai H, Vacanti JP (2003) A biodegradable nanofiber scaffold by electrospinning and its potential for bone tissue engineering. Biomaterials 24:2077–2082
Jiang W, Shi J, Li W, Sun K (2012) Morphology, wettability, and mechanical properties of polycaprolactone/hydroxyapatite composite scaffolds with interconnected pore structures fabricated by a mini-deposition system. Polym Eng Sci 52:2396–2402
Xu T, Miszuk JM, Zhao Y, Sun H, Fong H (2015) Electrospun polycaprolactone 3D nanofibrous scaffold with interconnected and hierarchically structured pores for bone tissue engineering. Adv Healthc Mater 4:2238–2246
Phipps MC, Clem WC, Grunda JM, Clines GA, Bellis SL (2012) Increasing the pore sizes of bone-mimetic electrospun scaffolds comprised of polycaprolactone, collagen I and hydroxyapatite to enhance cell infiltration. Biomaterials 33:524–534
Guo Z, Xu J, Ding S, Li H, Zhou C, Li L (2015) In vitro evaluation of random and aligned polycaprolactone/gelatin fibers via electrospinning for bone tissue engineering. J Biomater Sci Polym Ed 26:989–1001
Baker BM, Mauck RL (2007) The effect of nanofiber alignment on the maturation of engineered meniscus constructs. Biomaterials 28:1967–1977
Li T-T, Ebert K, Vogel J, Groth T (2013) Comparative studies on osteogenic potential of micro- and nanofibre scaffolds prepared by electrospinning of poly(ε-caprolactone). Prog Biomater 2:13
Scaglione S, Guarino V, Sandri M, Tampieri A, Ambrosio L, Quarto R (2012) In vivo lamellar bone formation in fibre coated MgCHA–PCL-composite scaffolds. J Mater Sci Mater Med 23:117–128
Chen X, Ergun A, Gevgilili H, Ozkan S, Kalyon DM, Wang H (2013) Shell-core bi-layered scaffolds for engineering of vascularized osteon-like structures. Biomaterials 34:8203–8212
Rong D, Chen P, Yang Y, Li Q, Wan W, Fang X et al (2016) Fabrication of gelatin/PCL electrospun fiber mat with bone powder and the study of its biocompatibility. J Funct Biomater 7:6
Qi H, Ye Z, Ren H, Chen N, Zeng Q, Wu X et al (2016) Bioactivity assessment of PLLA/PCL/HAP electrospun nanofibrous scaffolds for bone tissue engineering. Life Sci 148:139–144
Wutticharoenmongkol P, Pavasant P, Supaphol P (2007) Osteoblastic phenotype expression of MC3T3-E1 cultured on electrospun polycaprolactone fiber mats filled with hydroxyapatite nanoparticles. Biomacromolecules 8:2602–2610
Nandakumar A, Yang L, Habibovic P, van Blitterswijk C (2010) Calcium phosphate coated electrospun fiber matrices as scaffolds for bone tissue engineering. Langmuir 26:7380–7387
Yang F, Wolke JGC, Jansen JA (2008) Biomimetic calcium phosphate coating on electrospun poly(ɛ-caprolactone) scaffolds for bone tissue engineering. Chem Eng J 137:154–161
Thomas V, Jagani S, Johnson K, Jose MV, Dean DR, Vohra YK et al (2006) Electrospun bioactive nanocomposite scaffolds of polycaprolactone and nanohydroxyapatite for bone tissue engineering. J Nanosci Nanotechnol 6:487–493
Catledge SA, Clem WC, Shrikishen N, Chowdhury S, Stanishevsky AV, Koopman M et al (2007) An electrospun triphasic nanofibrous scaffold for bone tissue engineering. Biomed Mater 2:142
Yu H-S, Jang J-H, Kim T-I, Lee H-H, Kim H-W (2009) Apatite-mineralized polycaprolactone nanofibrous web as a bone tissue regeneration substrate. J Biomed Mater Res A 88A:747–754
Li X, Xie J, Yuan X, Xia Y (2008) Coating electrospun poly(ε-caprolactone) fibers with gelatin and calcium phosphate and their use as biomimetic scaffolds for bone tissue engineering. Langmuir 24:14145–14150
Nitya G, Nair GT, Mony U, Chennazhi KP, Nair SV (2012) In vitro evaluation of electrospun PCL/nanoclay composite scaffold for bone tissue engineering. J Mater Sci Mater Med 23:1749–1761
Ji W, Yang F, Ma J, Bouma MJ, Boerman OC, Chen Z et al (2013) Incorporation of stromal cell-derived factor-1α in PCL/gelatin electrospun membranes for guided bone regeneration. Biomaterials 34:735–745
Spadaccio C, Rainer A, Trombetta M, Vadalá G, Chello M, Covino E et al (2009) Poly-l-lactic acid/hydroxyapatite electrospun nanocomposites induce chondrogenic differentiation of human MSC. Ann Biomed Eng 37:1376–1389
Chen J, Chu B, Hsiao BS (2006) Mineralization of hydroxyapatite in electrospun nanofibrous poly(L-lactic acid) scaffolds. J Biomed Mater Res A 79A:307–317
Prabhakaran MP, Venugopal J, Ramakrishna S (2009) Electrospun nanostructured scaffolds for bone tissue engineering. Acta Biomater 5:2884–2893
Makadia HK, Siegel SJ (2011) Poly lactic-co-glycolic acid (PLGA) as biodegradable controlled drug delivery carrier. Polymers 3:1377
Lao L, Wang Y, Zhu Y, Zhang Y, Gao C (2011) Poly(lactide-co-glycolide)/hydroxyapatite nanofibrous scaffolds fabricated by electrospinning for bone tissue engineering. J Mater Sci Mater Med 22:1873–1884
Li D, Sun H, Jiang L, Zhang K, Liu W, Zhu Y et al (2014) Enhanced biocompatibility of PLGA nanofibers with gelatin/nano-hydroxyapatite bone biomimetics incorporation. ACS Appl Mater Interfaces 6:9402–9410
Lyu S, Huang C, Yang H, Zhang X (2013) Electrospun fibers as a scaffolding platform for bone tissue repair. J Orthop Res 31:1382–1389
Zhang H (2011) Electrospun poly (lactic-co-glycolic acid)/multiwalled carbon nanotubes composite scaffolds for guided bone tissue regeneration. J Bioact Compat Polym 26:347–362
Ito Y, Hasuda H, Kamitakahara M, Ohtsuki C, Tanihara M, Kang I-K et al (2005) A composite of hydroxyapatite with electrospun biodegradable nanofibers as a tissue engineering material. J Biosci Bioeng 100:43–49
Xie J, Willerth SM, Li X, Macewan MR, Rader A, Sakiyama-Elbert SE et al (2009) The differentiation of embryonic stem cells seeded on electrospun nanofibers into neural lineages. Biomaterials 30:354–362
Yeh L-C, Dai C-F, Yeh J-M, Hsieh P-Y, Wei Y, Chin T-Y et al (2013) Neat poly(ortho-methoxyaniline) electrospun nanofibers for neural stem cell differentiation. J Mater Chem B 1:5469–5477
Álvarez Z, Castaño O, Castells AA, Mateos-Timoneda MA, Planell JA, Engel E et al (2014) Neurogenesis and vascularization of the damaged brain using a lactate-releasing biomimetic scaffold. Biomaterials 35:4769–4781
Zhang K, Zheng H, Liang S, Gao C (2016) Aligned PLLA nanofibrous scaffolds coated with graphene oxide for promoting neural cell growth. Acta Biomater 37:131–142
Doyle AD, Yamada KM (2016) Mechanosensing via cell-matrix adhesions in 3D microenvironments. Exp Cell Res 343:60–66
Christopherson GT, Song H, Mao H-Q (2009) The influence of fiber diameter of electrospun substrates on neural stem cell differentiation and proliferation. Biomaterials 30:556–564
Wang A, Tang Z, Park I-H, Zhu Y, Patel S, Daley GQ et al (2011) Induced pluripotent stem cells for neural tissue engineering. Biomaterials 32:5023–5032
Panseri S, Cunha C, Lowery J, Del Carro U, Taraballi F, Amadio S et al (2008) Electrospun micro- and nanofiber tubes for functional nervous regeneration in sciatic nerve transections. BMC Biotechnol 8:39
Li W, Guo Y, Wang H, Shi D, Liang C, Ye Z et al (2008) Electrospun nanofibers immobilized with collagen for neural stem cells culture. J Mater Sci Mater Med 19:847–854
Cho YI, Choi JS, Jeong SY, Yoo HS (2010) Nerve growth factor (NGF)-conjugated electrospun nanostructures with topographical cues for neuronal differentiation of mesenchymal stem cells. Acta Biomater 6:4725–4733
Prabhakaran MP, Ghasemi-Mobarakeh L, Jin G, Ramakrishna S (2011) Electrospun conducting polymer nanofibers and electrical stimulation of nerve stem cells. J Biosci Bioeng 112:501–507
Lee JY, Bashur CA, Goldstein AS, Schmidt CE (2009) Polypyrrole-coated electrospun PLGA nanofibers for neural tissue applications. Biomaterials 30:4325–4335
Lins LC, Wianny F, Livi S, Hidalgo IA, Dehay C, Duchet-Rumeau J et al (2016) Development of bioresorbable hydrophilic–hydrophobic electrospun scaffolds for neural tissue engineering. Biomacromolecules 17:3172–3187
Mottaghitalab F, Farokhi M, Zaminy A, Kokabi M, Soleimani M, Mirahmadi F et al (2013) A biosynthetic nerve guide conduit based on silk/SWNT/fibronectin nanocomposite for peripheral nerve regeneration. PLoS One 8:e74417
Das S, Sharma M, Saharia D, Sarma KK, Sarma MG, Borthakur BB et al (2015) In vivo studies of silk based gold nano-composite conduits for functional peripheral nerve regeneration. Biomaterials 62:66–75
Wang G, Hu X, Lin W, Dong C, Wu H (2011) Electrospun PLGA–silk fibroin–collagen nanofibrous scaffolds for nerve tissue engineering. In Vitro Cell Dev Biol Anim 47:234–240
Prabhakaran MP, Venugopal JR, Ter Chyan T, Hai LB, Chan CK, Lim AY, Ramakrisha S (2008) Electrospun biocomposite nanofibrous scaffolds for neural tissue engineering. Tissue Eng Part A 14:1787–1797
Cooper A, Bhattarai N, Zhang M (2011) Fabrication and cellular compatibility of aligned chitosan–PCL fibers for nerve tissue regeneration. Carbohydr Polym 85:149–156
Prabhakaran MP, Vatankhah E, Ramakrishna S (2013) Electrospun aligned PHBV/collagen nanofibers as substrates for nerve tissue engineering. Biotechnol Bioeng 110:2775–2784
Huang C, Chen R, Ke Q, Morsi Y, Zhang K, Mo X (2011) Electrospun collagen–chitosan–TPU nanofibrous scaffolds for tissue engineered tubular grafts. Colloids Surf B: Biointerfaces 82:307–315
Baiguera S, Del Gaudio C, Lucatelli E, Kuevda E, Boieri M, Mazzanti B et al (2014) Electrospun gelatin scaffolds incorporating rat decellularized brain extracellular matrix for neural tissue engineering. Biomaterials 35:1205–1214
Han J, Wu Q, Xia Y, Wagner MB, Xu C (2016) Cell alignment induced by anisotropic electrospun fibrous scaffolds alone has limited effect on cardiomyocyte maturation. Stem Cell Res 16:740–750
Liu Q, Tian S, Zhao C, Chen X, Lei I, Wang Z et al (2015) Porous nanofibrous poly(l-lactic acid) scaffolds supporting cardiovascular progenitor cells for cardiac tissue engineering. Acta Biomater 26:105–114
Kai D, Prabhakaran MP, Jin G, Ramakrishna S (2011) Guided orientation of cardiomyocytes on electrospun aligned nanofibers for cardiac tissue engineering. J Biomed Mater Res B Appl Biomater 98B:379–386
Kang B-J, Kim H, Lee SK, Kim J, Shen Y, Jung S et al (2014) Umbilical-cord-blood-derived mesenchymal stem cells seeded onto fibronectin-immobilized polycaprolactone nanofiber improve cardiac function. Acta Biomater 10:3007–3017
Fleischer S, Feiner R, Shapira A, Ji J, Sui X, Daniel Wagner H et al (2013) Spring-like fibers for cardiac tissue engineering. Biomaterials 34:8599–8606
Tandon N, Cannizzaro C, Chao P-HG, Maidhof R, Marsano A, Au HTH et al (2009) Electrical stimulation systems for cardiac tissue engineering. Nat Protocol 4:155–173
Sridhar S, Venugopal JR, Sridhar R, Ramakrishna S (2015) Cardiogenic differentiation of mesenchymal stem cells with gold nanoparticle loaded functionalized nanofibers. Colloids Surf B: Biointerfaces 134:346–354
Kharaziha M, Shin SR, Nikkhah M, Topkaya SN, Masoumi N, Annabi N et al (2014) Tough and flexible CNT–polymeric hybrid scaffolds for engineering cardiac constructs. Biomaterials 35:7346–7354
Hsiao C-W, Bai M-Y, Chang Y, Chung M-F, Lee T-Y, Wu C-T et al (2013) Electrical coupling of isolated cardiomyocyte clusters grown on aligned conductive nanofibrous meshes for their synchronized beating. Biomaterials 34:1063–1072
Chung H-J, Kim J-T, Kim H-J, Kyung H-W, Katila P, Lee J-H et al (2015) Epicardial delivery of VEGF and cardiac stem cells guided by 3-dimensional PLLA mat enhancing cardiac regeneration and angiogenesis in acute myocardial infarction. J Control Release 205:218–230
Molamma PP, Dan K, Laleh G-M, Seeram R (2011) Electrospun biocomposite nanofibrous patch for cardiac tissue engineering. Biomed Mater 6:055001
Masoumi N, Annabi N, Assmann A, Larson BL, Hjortnaes J, Alemdar N et al (2014) Tri-layered elastomeric scaffolds for engineering heart valve leaflets. Biomaterials 35:7774–7785
Yang Liu YX, Zhenhua W, Dezhong W, Wentian Z, Sebastian S, Haiyan L, Yao C, Song X (2016) Electrospun nanofibrous sheets of collagen/elastin/polycaprolactone improve cardiac repair after myocardial infarction. Am J Transl Res 8(4):1678–1694
Meller D, Pauklin M, Thomasen H, Westekemper H, Steuhl K-P (2011) Amniotic membrane transplantation in the human eye. Dtsch Arztebl Int 108:243–248
Ye J, Shi X, Chen X, Xie J, Wang C, Yao K et al (2014) Chitosan-modified, collagen-based biomimetic nanofibrous membranes as selective cell adhering wound dressings in the treatment of chemically burned corneas. J Mater Chem B 2:4226–4236
Deshpande P, Ramachandran C, Sefat F, Mariappan I, Johnson C, McKean R et al (2013) Simplifying corneal surface regeneration using a biodegradable synthetic membrane and limbal tissue explants. Biomaterials 34:5088–5106
Biazar E, Baradaran-Rafii A, Heidari-keshel S, Tavakolifard S (2015) Oriented nanofibrous silk as a natural scaffold for ocular epithelial regeneration. J Biomater Sci Polym Ed 26:1139–1151
Tonsomboon K, Oyen ML (2013) Composite electrospun gelatin fiber-alginate gel scaffolds for mechanically robust tissue engineered cornea. J Mech Behav Biomed Mater 21:185–194
Ortega Í, Ryan AJ, Deshpande P, MacNeil S, Claeyssens F (2013) Combined microfabrication and electrospinning to produce 3-D architectures for corneal repair. Acta Biomater 9:5511–5520
Kong B, Sun W, Chen G, Tang S, Li M, Shao Z et al (2017) Tissue-engineered cornea constructed with compressed collagen and laser-perforated electrospun mat. Sci Rep 7:970
Cejkova J, Trosan P, Cejka C, Lencova A, Zajicova A, Javorkova E et al (2013) Suppression of alkali-induced oxidative injury in the cornea by mesenchymal stem cells growing on nanofiber scaffolds and transferred onto the damaged corneal surface. Exp Eye Res 116:312–323
Acun A, Hasirci V (2014) Construction of a collagen-based, split-thickness cornea substitute. J Biomater Sci Polym Ed 25:1110–1132
Sharma S, Gupta D, Mohanty S, Jassal M, Agrawal AK, Tandon R (2014) Surface-modified electrospun poly(ε-caprolactone) scaffold with improved optical transparency and bioactivity for damaged ocular surface reconstruction PCL scaffold in ocular surface engineering. Invest Ophthalmol Vis Sci 55:899–907
Tucker BA, Redenti SM, Jiang C, Swift JS, Klassen HJ, Smith ME et al (2010) The use of progenitor cell/biodegradable MMP2–PLGA polymer constructs to enhance cellular integration and retinal repopulation. Biomaterials 31:9–19
Zhang C, Wen J, Yan J, Kao Y, Ni Z, Cui X et al (2015) In situ growth induction of the corneal stroma cells using uniaxially aligned composite fibrous scaffolds. RSC Adv 5:12123–12130
Kobsa S, Kristofik NJ, Sawyer AJ, Bothwell ALM, Kyriakides TR, Saltzman WM (2013) An electrospun scaffold integrating nucleic acid delivery for treatment of full-thickness wounds. Biomaterials 34:3891–3901
Choi JS, Leong KW, Yoo HS (2008) In vivo wound healing of diabetic ulcers using electrospun nanofibers immobilized with human epidermal growth factor (EGF). Biomaterials 29:587–596
Huang R, Li W, Lv X, Lei Z, Bian Y, Deng H et al (2015) Biomimetic LBL structured nanofibrous matrices assembled by chitosan/collagen for promoting wound healing. Biomaterials 53:58–75
Rho KS, Jeong L, Lee G, Seo B-M, Park YJ, Hong S-D et al (2006) Electrospinning of collagen nanofibers: effects on the behavior of normal human keratinocytes and early-stage wound healing. Biomaterials 27:1452–1461
Min B-M, Lee G, Kim SH, Nam YS, Lee TS, Park WH (2004) Electrospinning of silk fibroin nanofibers and its effect on the adhesion and spreading of normal human keratinocytes and fibroblasts in vitro. Biomaterials 25:1289–1297
Kang YO, Yoon I-S, Lee SY, Kim D-D, Lee SJ, Park WH et al (2010) Chitosan-coated poly(vinyl alcohol) nanofibers for wound dressings. J Biomed Mater Res B Appl Biomater 92B:568–576
Yao C-H, Yeh J-Y, Chen Y-S, Li M-H, Huang C-H (2017) Wound-healing effect of electrospun gelatin nanofibres containing Centella asiatica extract in a rat model. J Tissue Eng Regen Med 11:905–915
Khil M-S, Cha D-I, Kim H-Y, Kim I-S, Bhattarai N (2003) Electrospun nanofibrous polyurethane membrane as wound dressing. J Biomed Mater Res B Appl Biomater 67B:675–679
Semnani D, Naghashzargar E, Hadjianfar M, Dehghan Manshadi F, Mohammadi S, Karbasi S et al (2017) Evaluation of PCL/chitosan electrospun nanofibers for liver tissue engineering. Int J Polym Mater Polym Biomater 66:149–157
Grant R, Hay DC, Callanan A (2017) A drug-induced hybrid electrospun poly-capro-lactone: cell-derived extracellular matrix scaffold for liver tissue engineering. Tissue Eng A 23:650–662
Naresh K, Utpal B (2012) Silk fibroin based biomimetic artificial extracellular matrix for hepatic tissue engineering applications. Biomed Mater 7:045004
Xu L, Wang S, Sui X, Wang Y, Su Y, Huang L et al (2017) Mesenchymal stem cell-seeded regenerated silk fibroin complex matrices for liver regeneration in an animal model of acute liver failure. ACS Appl Mater Interfaces 9:14716–14723
Liu Y, Li H, Yan S, Wei J, Li X (2014) Hepatocyte cocultures with endothelial cells and fibroblasts on micropatterned fibrous mats to promote liver-specific functions and capillary formation capabilities. Biomacromolecules 15:1044–1054
Kazemnejad S, Allameh A, Soleimani M, Gharehbaghian A, Mohammadi Y, Amirizadeh N et al (2009) Biochemical and molecular characterization of hepatocyte-like cells derived from human bone marrow mesenchymal stem cells on a novel three-dimensional biocompatible nanofibrous scaffold. J Gastroenterol Hepatol 24:278–287
Bishi DK, Guhathakurta S, Venugopal JR, Cherian KM, Ramakrishna S (2014) Low frequency magnetic force augments hepatic differentiation of mesenchymal stem cells on a biomagnetic nanofibrous scaffold. J Mater Sci Mater Med 25:2579–2589
Chen W, Chen S, Morsi Y, El-Hamshary H, El-Newhy M, Fan C et al (2016) Superabsorbent 3D scaffold based on electrospun nanofibers for cartilage tissue engineering. ACS Appl Mater Interfaces 8:24415–24425
Xu H, Cai S, Xu L, Yang Y (2014) Water-stable three-dimensional ultrafine fibrous scaffolds from keratin for cartilage tissue engineering. Langmuir 30:8461–8470
Alves da Silva ML, Martins A, Costa-Pinto AR, Costa P, Faria S, Gomes M et al (2010) Cartilage tissue engineering using electrospun PCL nanofiber meshes and MSCs. Biomacromolecules 11:3228–3236
Kim M, Hong B, Lee J, Kim SE, Kang SS, Kim YH et al (2012) Composite system of PLCL scaffold and heparin-based hydrogel for regeneration of partial-thickness cartilage defects. Biomacromolecules 13:2287–2298
Wang Z, Wang Y, Zhang P, Chen X (2015) Methylsulfonylmethane-loaded electrospun poly(lactide-co-glycolide) mats for cartilage tissue engineering. RSC Adv 5:96725–96732
Xue J, Feng B, Zheng R, Lu Y, Zhou G, Liu W et al (2013) Engineering ear-shaped cartilage using electrospun fibrous membranes of gelatin/polycaprolactone. Biomaterials 34:2624–2631
Subramanian A, Vu D, Larsen GF, Lin H-Y (2005) Preparation and evaluation of the electrospun chitosan/PEO fibers for potential applications in cartilage tissue engineering. J Biomater Sci Polym Ed 16:861–873
Deng J, Wang Y, Zhou L, Gou M, Luo N, Chen H et al (2015) Fabrication and in vivo chondrification of a poly(propylene carbonate)/l-lactide-grafted tetracalcium phosphate electrospun scaffold for cartilage tissue engineering. RSC Adv 5:42943–42954
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Nagarajan, S., Narayana Kalkura, S., Balme, S., Bohatier, C.P., Miele, P., Bechelany, M. (2019). Nanofibrous Scaffolds for Tissue Engineering Application. In: Barhoum, A., Bechelany, M., Makhlouf, A. (eds) Handbook of Nanofibers. Springer, Cham. https://doi.org/10.1007/978-3-319-53655-2_30
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