Abstract
Extrusion-based bioprinting is a powerful three-dimensional (3D) bioprinting technology that provides unique opportunities for use in organ fabrication. This technology has grown rapidly during the last decade. Extrusion-based bioprinting provides great versatility in printing various biological compounds or devices, including cells, tissues, organoids, and microfluidic devices that can be applied in basic research, pharmaceutics, drug testing, transplantation, and clinical uses. Extrusion-based bioprinting offers great flexibility in printing wide range of bioinks, including tissue spheroids, cell pellets, microcarriers, decellularized matrix components, and cell-laden hydrogels. Despite these assets, extrusion-based bioprinting has several limitations, such as inadequate control and resolution cell deposition, to create a complex tissue micro-microenvironment, shear stress-induced cell damage, and constraints associated with the current bioink materials.
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Achilli M, Mantovani D (2010) Tailoring mechanical properties of collagen-based scaffolds for vascular tissue engineering: the effects of pH, temperature and ionic strength on gelation. Polymers (Basel) 2:664–680. https://doi.org/10.3390/polym2040664
Achilli T, Meyer J, Morgan JR (2012) Advances in the formation, use and understanding of multi- cellular spheroids. Expert Opin Biol Ther 12:1347–1360. https://doi.org/10.1517/14712598.2012.707181.Advances
Ahn S, Lee H, Bonassar LJ, Kim G (2012a) Cells (MC3T3-E1)-laden alginate scaffolds fabricated by a modified solid-freeform fabrication process supplemented with an aerosol spraying. Biomacromolecules 13:2997–3003. https://doi.org/10.1021/bm3011352
Ahn S, Lee H, Puetzer J et al (2012b) Fabrication of cell-laden three-dimensional alginate-scaffolds with an aerosol cross-linking process. J Mater Chem 22:18735. https://doi.org/10.1039/c2jm33749e
Akkouch A, Yu Y, Ozbolat IT (2015) Microfabrication of scaffold-free tissue strands for three-dimensional tissue engineering. Biofabrication 7:31002. https://doi.org/10.1088/1758-5090/7/3/031002
Almeida C, Serra T, Oliveira M et al (2014) Impact of 3-D printed PLA- and chitosan-based scaffolds on human monocyte/macrophage responses: unraveling the effect of 3-D structures on inflammation. Acta Biomater 10:613–622. https://doi.org/10.1016/j.actbio.2013.10.035
Bammesberger SB, Kartmann S, Tanguy L et al (2013) A low-cost, normally closed, solenoid valve for non-contact dispensing in the sub-μl range. Micromachines 4:9–21. 10.3390/mi4010009
Bayram Y, Deveci M, Imirzalioglu N et al (2005) The cell based dressing with living allogenic keratinocytes in the treatment of foot ulcers: a case study. Br J Plast Surg 58:988–996. https://doi.org/10.1016/j.bjps.2005.04.031
Bertassoni LE, Cardoso JC, Manoharan V et al (2014) Direct-write bioprinting of cell-laden methacrylated gelatin hydrogels. Biofabrication 6:024105. https://doi.org/10.1088/1758-5082/6/2/024105
Billiet T, Gevaert E, De Schryver T et al (2014) The 3D printing of gelatin methacrylamide cell-laden tissue-engineered constructs with high cell viability. Biomaterials 35:49–62. https://doi.org/10.1016/j.biomaterials.2013.09.078
Boland T, Mironov V, Gutowska A et al (2003) Cell and organ printing 2: fusion of cell aggregates in three-dimensional gels. Anat Rec Part A Discov Mol Cell Evol Biol 272A:497–502. https://doi.org/10.1002/ar.a.10059
Breslin S, O’Driscoll L (2013) Three-dimensional cell culture: the missing link in drug discovery. Drug Discov Today 18:240–249. https://doi.org/10.1016/j.drudis.2012.10.003
Bruzewicz DA, Reches M, Whitesides GM (2012) Low-cost printing of PDMS barriers to define microchannels in paper. Changes 29:997–1003
Carrow JK, Gaharwar AK (2014) Bioinspired polymeric nanocomposites for regenerative medicine. Macromol Chem Phys. https://doi.org/10.1002/macp.201400427
Chang R, Nam J, Sun W (2008) Effects of dispensing pressure and nozzle diameter on cell survival from solid freeform fabrication-based direct cell writing. Tissue Eng Part A 14:41–48. https://doi.org/10.1089/ten.a.2007.0004
Chang CC, Boland ED, Williams SK, Hoying JB (2011) Direct-write bioprinting three-dimensional biohybrid systems for future regenerative therapies. J Biomed Mater Res B Appl Biomater 98:160–170. https://doi.org/10.1002/jbm.b.31831
Chung JHY, Naficy S, Yue Z et al (2013) Bio-ink properties and printability for extrusion printing living cells. Biomater Sci 1:763. https://doi.org/10.1039/c3bm00012e
Cohen DL, Lipton JI, Bonassar LJ, Lipson H (2010) Additive manufacturing for in situ repair of osteochondral defects. Biofabrication 2:035004. https://doi.org/10.1088/1758-5082/2/3/035004
Cohen J, Zaleski KL, Nourissat G et al (2011) Survival of porcine mesenchymal stem cells over the alginate recovered cellular method. J Biomed Mater Res A 96:93–99. https://doi.org/10.1002/jbm.a.32961
Cui X, Boland T (2009) Human microvasculature fabrication using thermal inkjet printing technology. Biomaterials 30:6221–6227. https://doi.org/10.1016/j.biomaterials.2009.07.056
Curran SJ, Chen R, Curran JM, Hunt J (2005) Expansion of human chondrocytes in an intermittent stirred flow bioreactor, using modified biodegradable microspheres. Tissue Eng 11:1312–1322. https://doi.org/10.1089/ten.2005.11.1312
Dababneh AB, Ozbolat IT (2014) Bioprinting technology: a current state-of-the-art review. J Manuf Sci Eng 136:061016. https://doi.org/10.1115/1.4028512
Dana N, Parker V, Meredith M et al (2004) Photocrosslinkable hyaluronan as a scaffold for articular cartilage repair. Ann Biomed Eng 32:391–397
Davoodi P, Feng F, Xu Q et al (2014) Coaxial electrohydrodynamic atomization: microparticles for drug delivery applications. J Control Release. https://doi.org/10.1016/j.jconrel.2014.12.004
Dean DM, Napolitano AP, Youssef J, Morgan JR (2007) Rods, tori, and honeycombs: the directed self-assembly of microtissues with prescribed microscale geometries. FASEB J 21:4005–4012. https://doi.org/10.1096/fj.07-8710com
Drury JL, Mooney DJ (2003) Hydrogels for tissue engineering: scaffold design variables and applications. Biomaterials 24:4337–4351. https://doi.org/10.1016/S0142-9612(03)00340-5
Duan B, Hockaday L, Kang KH, Butcher JT (2013) 3D bioprinting of heterogeneous aortic valve conduits with alginate/gelatin hydrogels. J Biomed Mater Res Part A 101 A:1255–1264. https://doi.org/10.1002/jbm.a.34420
Duarte Campos DF, Blaeser A, Weber M et al (2013) Three-dimensional printing of stem cell-laden hydrogels submerged in a hydrophobic high-density fluid. Biofabrication 5:015003. https://doi.org/10.1088/1758-5082/5/1/015003
Duarte Campos DF, Blaeser A, Korsten A et al (2014) The stiffness and structure of three-dimensional printed hydrogels direct the differentiation of mesenchymal stromal cells toward adipogenic and osteogenic lineages. Tissue Eng Part A:1–17. https://doi.org/10.1089/ten.TEA.2014.0231
Ehsan SM, Welch-Reardon KM, Waterman ML et al (2014) A three-dimensional in vitro model of tumor cell intravasation. Integr Biol (Camb) 6:603–610. https://doi.org/10.1039/c3ib40170g
Elbert DL (2012) Liquid-liquid two phase systems for the production of porous hydrogels and hydrogel microspheres for biomedical applications: a tutorial review. Acta Biomater 7:31–56. https://doi.org/10.1016/j.actbio.2010.07.028.Liquid-liquid
Fedorovich NE, Alblas J, de Wijn JR et al (2007) Hydrogels as extracellular matrices for skeletal tissue engineering: state-of-the-art and novel application in organ printing. Tissue Eng 13:1905–1925. https://doi.org/10.1089/ten.2006.0175
Fedorovich NE, De Wijn JR, Verbout AJ et al (2008) Three-dimensional fiber deposition of cell-laden, viable, patterned constructs for bone tissue printing. Tissue Eng Part A 14:127–133. https://doi.org/10.1089/ten.a.2007.0158
Fedorovich NE, Wijnberg HM, Dhert WJ, Alblas J (2011) Distinct tissue formation by heterogeneous printing of osteo- and endothelial progenitor cells. Tissue Eng Part A 17:2113–2121. https://doi.org/10.1089/ten.tea.2011.0019
Fielding G, Bandyopadhyay A, Bose S (2012) Effects of silica and zinc oxide doping on mechanical and biological properties of 3D printed tricalcium phosphate tissue engineering scaffolds. Dent Mater 28:113–122. https://doi.org/10.1016/j.dental.2011.09.010
Geng L, Feng W, Hutmacher DW et al (2005) Direct writing of chitosan scaffolds using a robotic system. Rapid Prototyp J 11:90–97. https://doi.org/10.1108/13552540510589458
Gerecht S, Burdick J, Ferreira LS et al (2007) Hyaluronic acid hydrogel for controlled self-renewal and differentiation of human embryonic stem cells. Proc Natl Acad Sci U S A 104:11298–11303. https://doi.org/10.1073/pnas.0703723104
Giovagnoli S, Tsai T, DeLuca PP (2010) Formulation and release behavior of doxycycline-alginate hydrogel microparticles embedded into pluronic F127 thermogels as a potential new vehicle for doxycycline intradermal sustained delivery. AAPS PharmSciTech 11:212–220. https://doi.org/10.1208/s12249-009-9361-8
Gómez-Guillén MC, Giménez B, López-Caballero ME, Montero MP (2011) Functional and bioactive properties of collagen and gelatin from alternative sources: a review. Food Hydrocoll 25:1813–1827. https://doi.org/10.1016/j.foodhyd.2011.02.007
Gou M, Qu X, Zhu W et al (2014) Bio-inspired detoxification using 3D-printed hydrogel nanocomposites. Nat Commun 5:3774. https://doi.org/10.1038/ncomms4774
Gregor A, Hošek J (2011) 3D printing methods of biological scaffolds used in tissue engineering. Rom Rev Precis Mech Opt Mechatronics 3:143–148
Grzesik WJ, Robey PG (1994) Bone matrix RGD glycoproteins: immunolocalization bone cells in vitro. J Bone Miner Res 9:487–496
Hao T, Wen N, Cao JK et al (2010) The support of matrix accumulation and the promotion of sheep articular cartilage defects repair in vivo by chitosan hydrogels. Osteoarthr Cartil 18:257–265. https://doi.org/10.1016/j.joca.2009.08.007
Hockaday L, Kang KH, Colangelo NW et al (2012) Rapid 3D printing of anatomically accurate and mechanically heterogeneous aortic valve hydrogel scaffolds. Biofabrication 4:035005. https://doi.org/10.1088/1758-5082/4/3/035005
Homenick CM, de Silveira G, Sheardown H, Adronov A (2011) Pluronics as crosslinking agents for collagen: novel amphiphilic hydrogels. Polym Int 60:458–465. https://doi.org/10.1002/pi.2969
Hong Y, Song H, Gong Y et al (2007) Covalently crosslinked chitosan hydrogel: properties of in vitro degradation and chondrocyte encapsulation. Acta Biomater 3:23–31. https://doi.org/10.1016/j.actbio.2006.06.007
Hsiao AY, Torisawa YS, Tung YC et al (2009) Microfluidic system for formation of PC-3 prostate cancer co-culture spheroids. Biomaterials 30:3020–3027. https://doi.org/10.1016/j.biomaterials.2009.02.047
Hussain I, Hussain SZ, Habib-ur-Rehman et al (2010) In situ growth of gold nanoparticles on latent fingerprints-from forensic applications to inkjet printed nanoparticle patterns. Nanoscale 2:2575–2578. https://doi.org/10.1039/c0nr00593b
Jakab K, Norotte C, Marga F et al (2010) Tissue engineering by self-assembly and bio-printing of living cells. Biofabrication 2:22001
Jakob PH, Kehrer J, Flood P et al (2016) A 3-D cell culture system to study epithelia functions using microcarriers. Cytotechnology 1–13. https://doi.org/10.1007/s10616-015-9935-0
Jang J, Kim TG, Kim BS et al (2016) Tailoring mechanical properties of decellularized extracellular matrix bioink by vitamin B2-induced photo-crosslinking. Acta Biomater 33:88–95. https://doi.org/10.1016/j.actbio.2016.01.013
Jeon O, Song SJ, Lee K-J et al (2007) Mechanical properties and degradation behaviors of hyaluronic acid hydrogels cross-linked at various cross-linking densities. Carbohydr Polym 70:251–257. https://doi.org/10.1016/j.carbpol.2007.04.002
Jia J, Richards DJ, Pollard S et al (2014) Engineering alginate as bioink for bioprinting. Acta Biomater 10:4323–4331. https://doi.org/10.1016/j.actbio.2014.06.034
Jungst T, Smolan W, Schacht K et al (2016) Strategies and molecular design criteria for 3D printable hydrogels. Chem Rev 116:1496–1539. https://doi.org/10.1021/acs.chemrev.5b00303
Katakai T, Hara T, Lee JH et al (2004) A novel reticular stromal structure in lymph node cortex: an immuno-platform for interactions among dendritic cells, T cells and B cells. Int Immunol 16:1133–1142. https://doi.org/10.1093/intimm/dxh113
Ker EDF, Chu B, Phillippi J et al (2011) Engineering spatial control of multiple differentiation fates within a stem cell population. Biomaterials 32:3413–3422. https://doi.org/10.1016/j.biomaterials.2011.01.036
Kesti M, Eberhardt C, Pagliccia G et al (2015) Bioprinting complex cartilaginous structures with clinically compliant biomaterials. Adv Funct Mater 25:7406–7417. https://doi.org/10.1002/adfm.201503423
Khalil S, Sun W (2009) Bioprinting endothelial cells with alginate for 3D tissue constructs. J Biomech Eng 131:111002. https://doi.org/10.1115/1.3128729
Khalil S, Nam J, Sun W (2005) Multi-nozzle deposition for construction of 3-D biopolymer tissue scaffolds. Rapid Prototyp J 11:9–17
Khoda A, Ozbolat IT, Koc B (2011) Engineered tissue scaffolds with variational porous architecture. J Biomech Eng 133:011001. https://doi.org/10.1115/1.4002933
Kleinman HK, Martin GR (2005) Matrigel: basement membrane matrix with biological activity. Semin Cancer Biol 15:378–386. https://doi.org/10.1016/j.semcancer.2005.05.004
Kobayashi K, Huang C, Lodge TP (1999) Thermoreversible gelation of aqueous methylcellulose solutions. Macromolecules 32:7070–7077. https://doi.org/10.1021/ma990242n
Landers R, Hübner U, Schmelzeisen R, Mülhaupt R (2002) Rapid prototyping of scaffolds derived from thermoreversible hydrogels and tailored for applications in tissue engineering. Biomaterials 23:4437–4447. https://doi.org/10.1016/S0142-9612(02)00139-4
Lee W, Lee V, Polio S et al (2010a) On-demand three-dimensional freeform fabrication of multi-layered hydrogel scaffold with fluidic channels. Biotechnol Bioeng 105:1178–1186. https://doi.org/10.1002/bit.22613
Lee Y-B, Polio S, Lee W et al (2010b) Bio-printing of collagen and VEGF-releasing fibrin gel scaffolds for neural stem cell culture. Exp Neurol 223:645–652. https://doi.org/10.1016/j.expneurol.2010.02.014
Levato R, Visser J, Planell J et al (2014) Biofabrication of tissue constructs by 3D bioprinting of cell-laden microcarriers. Biofabrication 6:35020
Li C, Faulkner-Jones A, Dun AR et al (2015a) Rapid formation of a supramolecular polypeptide-DNA hydrogel for in situ three-dimensional multilayer bioprinting. Angew Chem Int Ed Engl 1–6. https://doi.org/10.1002/anie.201411383
Li H, Dai Y, Shu J, et al (2015b) Spheroid cultures promote the stemness of corneal stromal cells. Tissue Cell 47:39–48. https://doi.org/10.1016/j.tice.2014.10.008
Liu JY, Hafner J, Dragieva G et al (2004) Autologous cultured keratinocytes on porcine gelatin microbeads effectively heal chronic venous leg ulcers. Wound Repair Regen 12:148–156. https://doi.org/10.1111/j.1067-1927.2004.012205.x
Lopes AJ, Lee IH, Macdonald E et al (2014) Laser curing of silver-based conductive inks for in situ 3D structural electronics fabrication in stereolithography. J Mater Process Technol 214:1935–1945. https://doi.org/10.1016/j.jmatprotec.2014.04.009
Lott JR, McAllister JW, Arvidson S et al (2013) Fibrillar structure of methylcellulose hydrogels. Biomacromolecules 14:2484–2488. https://doi.org/10.1021/bm400694r
Ma L, Gao C, Mao Z et al (2003) Collagen/chitosan porous scaffolds with improved biostability for skin tissue engineering. Biomaterials 24:4833–4841. https://doi.org/10.1016/S0142-9612(03)00374-0
Malda J, van Blitterswijk C, Grojec M et al (2003) Expansion of bovine chondrocytes on microcarriers enhances redifferentiation. Tissue Eng 9:939–948. https://doi.org/10.1089/107632703322495583
Malda J, Visser J, Melchels FP et al (2013) 25th anniversary article: engineering hydrogels for biofabrication. Adv Mater 25:5011–5028. https://doi.org/10.1002/adma.201302042
Markstedt K, Mantas A, Tournier I et al (2015) 3D bioprinting human chondrocytes with nanocellulose-alginate bioink for cartilage tissue engineering applications. Biomacromolecules 150325142328001. https://doi.org/10.1021/acs.biomac.5b00188
Mehesz AN, Brown J, Hajdu Z et al (2011) Scalable robotic biofabrication of tissue spheroids. Biofabrication 3:25002
Melchels FPW, Dhert WJ, Hutmacher DW, Malda J (2014) Development and characterisation of a new bioink for additive tissue manufacturing. J Mater Chem B 2:2282. https://doi.org/10.1039/c3tb21280g
Melchels FPW, Blokzijl MM, Levato R et al (2016) Hydrogel-based reinforcement of 3D bioprinted constructs. Biofabrication 8:035004. https://doi.org/10.1088/1758-5090/8/3/035004
Mewis J, Wagner NJ (2009) Thixotropy. Adv Colloid Interface Sci 147–148:214–227. https://doi.org/10.1016/j.cis.2008.09.005
Mironov V, Boland T, Trusk T et al (2003) Organ printing: computer-aided jet-based 3D tissue engineering. Trends Biotechnol 21:157–161. https://doi.org/10.1016/S0167-7799(03)00033-7
Mironov V, Visconti RP, Kasyanov V et al (2009) Organ printing: tissue spheroids as building blocks. Biomaterials 30:2164–2174
Mironov V, Kasyanov V, Markwald RR (2011) Organ printing: from bioprinter to organ biofabrication line. Curr Opin Biotechnol 22:667–673. https://doi.org/10.1016/j.copbio.2011.02.006
Mogas-Soldevila L, Duro-Royo J, Oxman N (2014) Water-based robotic fabrication: large-scale additive manufacturing of functionally graded hydrogel composites via multichamber extrusion. 3D Print Addit Manuf 1:141–151. https://doi.org/10.1089/3dp.2014.0014
Mooney R, Haeger S, Lawal R et al (2011) Control of neural cell composition in poly(ethylene glycol) hydrogel culture with soluble factors. Tissue Eng Part A 17:2805–2815. https://doi.org/10.1089/ten.tea.2010.0654
Müller M, Becher J, Schnabelrauch M, Zenobi-Wong M (2015) Nanostructured pluronic hydrogels as bioinks for 3D bioprinting. Biofabrication 7:035006. https://doi.org/10.1088/1758-5090/7/3/035006
Murphy SV, Skardal A, Atala A (2013) Evaluation of hydrogels for bio-printing applications. J Biomed Mater Res Part A 101(A):272–284. https://doi.org/10.1002/jbm.a.34326
Norotte C, Marga FS, Niklason LE, Forgacs G (2009) Scaffold-free vascular tissue engineering using bioprinting. Biomaterials 30:5910–5917. https://doi.org/10.1016/j.biomaterials.2009.06.034
Odde DJ, Renn MJ (2000) Laser-guided direct writing of living cells. Biotechnol Bioeng 67:312
Ong SY, Wu J, Moochhala SM et al (2008) Development of a chitosan-based wound dressing with improved hemostatic and antimicrobial properties. Biomaterials 29:4323–4332. https://doi.org/10.1016/j.biomaterials.2008.07.034
Overstreet M, Sohrabi A, Polotsky A et al (2003) Collagen microcarrier spinner culture promotes osteoblast proliferation and synthesis of matrix proteins. In Vitro Cell Dev Biol Anim 39:228–234. https://doi.org/10.1290/1543-706X(2003)039
Oxlund H, Andreassen TT (1980) The roles of hyaluronic acid, collagen and elastin in the mechanical properties of connective tissues. J Anat 131:611–620
Ozbolat IT (2015a) Bioprinting scale-up tissue and organ constructs for transplantation. Trends Biotechnol 33:395–400. https://doi.org/10.1016/j.tibtech.2015.04.005
Ozbolat IT (2015b) Scaffold-based or scaffold-free bioprinting: competing or complementing approaches? J Nanotechnol Eng Med. https://doi.org/10.1115/1.4030414
Ozbolat IT, Hospodiuk M (2016) Current advances and future perspectives in extrusion-based bioprinting. Biomaterials 76:321–343. https://doi.org/10.1016/j.biomaterials.2015.10.076
Ozbolat IT, Koc B (2010) Modeling of spatially controlled biomolecules in three-dimensional porous alginate structures. J Med Devices 4:041003. https://doi.org/10.1115/1.4002612
Ozbolat IT, Koc B (2011) Multi-function based modeling of 3D heterogeneous wound scaffolds for improved wound healing. Comput Aided Des Applic 8:43–57. https://doi.org/10.3722/cadaps.2011.43-57
Ozbolat IT, Chen H, Yu Y (2014) Development of “multi-arm bioprinter” for hybrid biofabrication of tissue engineering constructs. Robot Comput Integr Manuf 30:295–304. https://doi.org/10.1016/j.rcim.2013.10.005
Pardo L, Wilson WC, Boland T (2003) Characterization of patterned self-assembled monolayers and protein arrays generated by the ink-jet method. Langimur 19:1462–1466
Park JY, Choi J-C, Shim J-H et al (2014) A comparative study on collagen type I and hyaluronic acid dependent cell behavior for osteochondral tissue bioprinting. Biofabrication 6:035004. https://doi.org/10.1088/1758-5082/6/3/035004
Pati F, Jang J, Ha D-H et al (2014) Printing three-dimensional tissue analogues with decellularized extracellular matrix bioink. Nat Commun 5:3935. https://doi.org/10.1038/ncomms4935
Pati F, Ha D, Jang J et al (2015a) Biomimetic 3D tissue printing for soft tissue regeneration. Biomaterials 62:164–175. https://doi.org/10.1016/j.biomaterials.2015.05.043
Pati F, Jang J, Lee JW, Cho DW (2015b) Extrusion bioprinting. Essentials of 3D biofabrication and translation. https://doi.org/10.1016/B978-0-12-800972-7.00007-4
Pfister A, Landers R, Laib A et al (2004) Biofunctional rapid prototyping for tissue-engineering applications: 3D bioplotting versus 3D printing. J Polym Sci Part A Polym Chem 42:624–638. https://doi.org/10.1002/pola.10807
Poldervaart MT, Wang H, van der Stok J et al (2013) Sustained release of BMP-2 in bioprinted alginate for osteogenicity in mice and rats. PLoS One 8:e72610. https://doi.org/10.1371/journal.pone.0072610
Ren X, Kim Y, Zhou J (2013) Design and fabrication of chitosan for application of artificial photosynthesis. J Mech Eng Automat 3:739–746
Rinaudo M (2006) Chitin and chitosan: properties and applications. Prog Polym Sci 31:603–632. https://doi.org/10.1016/j.progpolymsci.2006.06.001
Rowley JA, Madlambayan G, Mooney DJ (1999) Alginate hydrogels as synthetic extracellular matrix materials. Biomaterials 20:45–53. https://doi.org/10.1016/S0142-9612(98)00107-0
Rücker M, Laschke MW, Junker D et al (2006) Angiogenic and inflammatory response to biodegradable scaffolds in dorsal skinfold chambers of mice. Biomaterials 27:5027–5038. https://doi.org/10.1016/j.biomaterials.2006.05.033
Serwer P, Allen JL, Hayes SJ (1983) Agarose gel electrophoresis of bacteriophages and related particles III. Dependence of gel sieving on the agarose preparation. Electrophoresis 4:232–236. https://doi.org/10.1002/elps.1150040309
Shikani AH, Fink DJ, Sohrabi A et al (2004) Propagation of human nasal chondrocytes in microcarrier spinner culture. Am J Rhinol 18:105–112
Skardal A, Atala A (2014) Biomaterials for integration with 3-D bioprinting. Ann Biomed Eng. https://doi.org/10.1007/s10439-014-1207-1
Skardal A, Sarker SF, Crabbé A et al (2010a) The generation of 3-D tissue models based on hyaluronan hydrogel-coated microcarriers within a rotating wall vessel bioreactor. Biomaterials 31:8426–8435. https://doi.org/10.1016/j.biomaterials.2010.07.047
Skardal A, Zhang J, McCoard L et al (2010b) Photocrosslinkable hyaluronan-gelatin hydrogels for two-step bioprinting. Tissue Eng Part A 16:2675–2685. https://doi.org/10.1089/ten.TEA.2009.0798
Smith CM, Stone A, Stewart RL et al (2004) Three-dimensional bioassembly tool for generating viable tissue-engineered constructs. Tissue Eng 10:1566–1576
Snyder JE, Hamid Q, Wang C et al (2011) Bioprinting cell-laden matrigel for radioprotection study of liver by pro-drug conversion in a dual-tissue microfluidic chip. Biofabrication 3:034112. https://doi.org/10.1088/1758-5082/3/3/034112
Thirumala S, Gimble J, Devireddy R (2013) Methylcellulose based thermally reversible hydrogel system for tissue engineering applications. Cells 2:460–475. https://doi.org/10.3390/cells2030460
Torisawa Y, Mosadegh B, Luker GD et al (2009) Microfluidic hydrodynamic cellular patterning for systematic formation of co-culture spheroids. Integr Biol (Camb) 1:649–654. https://doi.org/10.1039/b915965g
Trojani C, Weiss P, Michiels JF et al (2005) Three-dimensional culture and differentiation of human osteogenic cells in an injectable hydroxypropylmethylcellulose hydrogel. Biomaterials 26:5509–5517. https://doi.org/10.1016/j.biomaterials.2005.02.001
Van Den Bulcke I, Bogdanov B, De Rooze N et al (2000) Structural and rheological properties of methacrylamide modified gelatin hydrogels. Biomacromolecules 1:31–38. https://doi.org/10.1021/bm990017d
Veronese FM, Pasut G (2005) PEGylation, successful approach to drug delivery. Drug Discov Today 10:1451–1458. https://doi.org/10.1016/S1359-6446(05)03575-0
Vinatier C, Magne D, Weiss P et al (2005) A silanized hydroxypropyl methylcellulose hydrogel for the three-dimensional culture of chondrocytes. Biomaterials 26:6643–6651. https://doi.org/10.1016/j.biomaterials.2005.04.057
Vozzi G, Previti A, De Rossi D, Ahluwalia A (2002) Microsyringe-based deposition of two-dimensional and three-dimensional polymer scaffolds with a well-defined geometry for application to tissue engineering. Tissue Eng 8:1089–1098. https://doi.org/10.1089/107632702320934182
Wang X, Yan Y, Pan Y et al (2006) Generation of three-dimensional hepatocyte/gelatin structures with rapid prototyping system. Tissue Eng 12:83–90. https://doi.org/10.1089/ten.2006.12.ft-16
Wang Z, Wu W, Yang Q et al (2009) In-situ fabrication of flexible vertically integrated electronic circuits by inkjet printing. J Alloys Compd 486:706–710. https://doi.org/10.1016/j.jallcom.2009.07.044
Wei Xu, Xiaohong Wang, Yongnian Yan, et al (2007) Rapid prototyping three-dimensional cell/gelatin/fibrinogen constructs for medical regeneration. J Bioact Compat Polym 22:363–377. https://doi.org/10.1177/0883911507079451
Wu PK, Ringeisen BR (2010) Development of human umbilical vein endothelial cell (HUVEC) and human umbilical vein smooth muscle cell (HUVSMC) branch/stem structures on hydrogel layers via biological laser printing (BioLP). Biofabrication 2:014111. https://doi.org/10.1088/1758-5082/2/1/014111
Wu C, Luo Y, Cuniberti G et al (2011a) Three-dimensional printing of hierarchical and tough mesoporous bioactive glass scaffolds with a controllable pore architecture, excellent mechanical strength and mineralization ability. Acta Biomater 7:2644–2650. https://doi.org/10.1016/j.actbio.2011.03.009
Wu W, DeConinck A, Lewis J (2011b) Omnidirectional printing of 3D microvascular networks. Adv Mater 23:H178–H183. https://doi.org/10.1002/adma.201004625
Wüst S, Müller R, Hofmann S (2014) 3D bioprinting of complex channels – effects of material, orientation, geometry and cell embedding. J Biomed Mater Res A. https://doi.org/10.1002/jbm.a.35393
Xing Q, Yates K, Vogt C et al (2014) Increasing mechanical strength of gelatin hydrogels by divalent metal ion removal. Sci Rep 4:4706. https://doi.org/10.1038/srep04706
Xu T, Gregory C, Molnar P et al (2006) Viability and electrophysiology of neural cell structures generated by the inkjet printing method. Biomaterials 27:3580–3588. https://doi.org/10.1016/j.biomaterials.2006.01.048
Yang S, Leong K-F, Du Z, Chua C-K (2002) The design of scaffolds for use in tissue engineering. Part II. Rapid prototyping techniques. Tissue Eng 8:1–11. https://doi.org/10.1089/107632702753503009
Ye K, Felimban R, Traianedes K et al (2014) Chondrogenesis of infrapatellar fat pad derived adipose stem cells in 3D printed chitosan scaffold. PLoS One. https://doi.org/10.1371/journal.pone.0099410
Yu Y, Ozbolat IT (2014) Tissue strands as “bioink” for scale-up organ printing. Conf Proc IEEE Eng Med Biol Soc 2014:1428–1431. https://doi.org/10.1109/EMBC.2014.6943868
Yu Y, Brouillette MJ, Seol D et al (2012) Functional full-thickness articular cartilage repair by rhSDF-1α loaded fibrin/HA hydrogel network via chondrogenic progenitor cells homing. Arthritis Rheum 1–30. https://doi.org/10.1002/art
Yu Y, Zhang Y, Martin J, Ozbolat IT (2013) Evaluation of cell viability and functionality in vessel-like bioprintable cell-laden tubular channels. J Biomech Eng 135:91011. https://doi.org/10.1115/1.4024575
Yu Y, Zhang Y, Ozbolat IT (2014) A hybrid bioprinting approach for scale-up tissue fabrication. J Manuf Sci Eng 136:061013. https://doi.org/10.1115/1.4028511
Zhang M, Desai T, Ferrari M (1998) Proteins and cells on PEG immobilized silicon surfaces. Biomaterials 19:953–960. https://doi.org/10.1016/S0142-9612(98)00026-X
Zhang Y, Venugopal JR, El-Turki A et al (2008) Electrospun biomimetic nanocomposite nanofibers of hydroxyapatite/chitosan for bone tissue engineering. Biomaterials 29:4314–4322. https://doi.org/10.1016/j.biomaterials.2008.07.038
Zhang L, Huang J, Si T, Xu RX (2012) Coaxial electrospray of microparticles and nanoparticles for biomedical applications. Changes 29:997–1003
Zhang Y, Yu Y, Ozbolat IT (2013) Direct bioprinting of vessel-like tubular microfluidic channels. J Nanotechnol Eng Med 4:020902. https://doi.org/10.1115/1.4024398
Zhang Y, Yu Y, Akkouch A et al (2015) In vitro study of directly bioprinted perfusable vasculature conduits. Biomater Sci 3:134–143. https://doi.org/10.1039/C4BM00234B
Acknowledgments
This work has been supported by National Science Foundation CMMI Awards 1349716 and 1462232. We thank Fisayo Olashore and Donna Sosnoski from the Pennsylvania State University for improving the quality of the paper. The authors are grateful to the support from the Engineering Science and Mechanics Department and the College of Engineering at the Penn State University. The authors confirm that there are no known conflicts of interest associated with this publication, and there has been no significant financial support for this work that could have influenced its outcome.
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Hospodiuk, M., Moncal, K.K., Dey, M., Ozbolat, I.T. (2018). Extrusion-Based Biofabrication in Tissue Engineering and Regenerative Medicine. In: Ovsianikov, A., Yoo, J., Mironov, V. (eds) 3D Printing and Biofabrication. Reference Series in Biomedical Engineering(). Springer, Cham. https://doi.org/10.1007/978-3-319-45444-3_10
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