Abstract
Most published methods for bacteriophage culture and purification have been developed around the needs of laboratory-scale production. As the use of phages as antibacterials continues to develop, phages infecting a diverse array of bacteria will need to be produced and purified economically at large scales. Historically, the hosts used for the propagation of phages infecting pathogenic bacteria have been selected largely out of necessity, but the choice of phage propagation hosts can influence the safety, economics, and scalability of phage cultures. This chapter will cover some of the basics of phage propagation and the means by which the choice of propagation host may improve culture yield, biological safety, and downstream purification processes.
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Gill, J.J. (2020). The Selection and Optimization of Phage Hosts. In: Harper, D., Abedon, S., Burrowes, B., McConville, M. (eds) Bacteriophages. Springer, Cham. https://doi.org/10.1007/978-3-319-40598-8_24-1
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DOI: https://doi.org/10.1007/978-3-319-40598-8_24-1
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