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Cell Culture Conditions: Cultivation of Stem Cells Under Dynamic Conditions

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Book cover Cell Engineering and Regeneration

Part of the book series: Reference Series in Biomedical Engineering ((TIENRE))

Abstract

A major challenge in tissue engineering and cell-therapy-based approaches is the production of a significant amount of functional cells. Although it does not represent a physiologic environment, most of stem cell cultivation is performed in static conditions (e.g., petri dishes). Furthermore, static expansion or differentiation of stem cells is labor-intensive and cells are often limited in number and functionality. In contrast, dynamic conditions (intentional active motion) enhance mass transfer and mechanotransductive effects which often results in higher numbers of functional cells. Specialized and partially automated bioreactor systems are widely used to transfer motion to cells and monitor important cultivation parameters. Furthermore, in dynamic differentiation processes, bioreactors directly apply mechanical forces to generate physiologic conditions and enhance differentiation towards a specific lineage. Therefore, in this chapter, we discuss the application of dynamic conditions for the expansion and differentiation of stem cells. Consequently, a comprehensive overview of commercially available bioreactors for the expansion and differentiation of stem cells is presented.

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Authors and Affiliations

  1. Department of Biotechnology, University of Natural Resources and Life Sciences, Vienna, Austria

    Andreas Clementi, Dominik Egger, Verena Charwat & Cornelia Kasper

Authors
  1. Andreas Clementi
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  2. Dominik Egger
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  3. Verena Charwat
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  4. Cornelia Kasper
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Corresponding author

Correspondence to Cornelia Kasper .

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Editors and Affiliations

  1. Tulane Univ. Sch. of Medicine, New Orleans, Louisiana, USA

    Jeffrey M. Gimble

  2. AUVA Center, Ludwig Boltzmann Institute for Experimen, Vienna, Austria

    Darja Marolt

  3. Human Development and Health, University of Southampton, Southampton, United Kingdom

    Richard Oreffo

  4. Experimental and Clinical Traumatol, Ludwig Boltzmann Institute for, Wien, Austria

    Heinz Redl

  5. AUVA Center, Ludwig Boltzmann Institute for Experimen, Vienna, Austria

    Susanne Wolbank

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Clementi, A., Egger, D., Charwat, V., Kasper, C. (2018). Cell Culture Conditions: Cultivation of Stem Cells Under Dynamic Conditions. In: Gimble, J., Marolt, D., Oreffo, R., Redl, H., Wolbank, S. (eds) Cell Engineering and Regeneration. Reference Series in Biomedical Engineering(). Springer, Cham. https://doi.org/10.1007/978-3-319-37076-7_58-1

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  • DOI: https://doi.org/10.1007/978-3-319-37076-7_58-1

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