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Metabolic Engineering of Lignan Biosynthesis Pathways for the Production of Transgenic Plant-Based Foods

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Transgenesis and Secondary Metabolism

Part of the book series: Reference Series in Phytochemistry ((RSP))

Abstract

Lignans are major phytochemicals biosynthesized in several plants including Sesamum, Linum, Forsythia, and Podophyllum genus, and a great variety of lignans have received wide attentions as leading compounds of novel drugs for tumor treatment and healthy diets to reduce of the risks of lifestyle-related diseases. Recent genome and transcriptome studies have characterized multiple novel lignan-biosynthetic enzymes, and thus have opened new avenues to transgenic metabolic engineering of various nonmodel dietary or medicinal plants. Forsythia and Linum are the most useful and prevalent natural and agricultural sources for the development of both transgenic foods and medicinal compounds. Over the past few years, transiently gene-transfected or transgenic Forsythia and Linum plants or cell cultures have been shown to be promising platforms for the sustainable and efficient production of beneficial lignans. In this chapter, we present the essential knowledge and recent advances regarding metabolic engineering of lignans based on their biosynthetic pathways and biological activities and the perspectives in lignan production via metabolic engineering.

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Abbreviations

CAD:

Cinnamylalcohol dehydrogenase

CCR:

Cinnamoyl-CoA reductase

DIR:

Dirigent protein

ER:

Estrogen receptor

MAPK:

Mitogen-activated protein kinase

MeJA:

Methyl jasmonate

MOMT:

Matairesinol O-methyltransferase

PAL:

Phenylalanine ammonialyase

PIP:

Pinoresinol-lariciresinol/isoflavone/phenylcoumaran benzylic ether reductase

PLR:

Pinoresinol-lariciresinol reductase

PTOX:

Podophyllotoxin

RNAi:

RNA interference

SA:

Salicylic acid

SDG:

Secoisolariciresinol diglucoside

SIRD:

Secoisolariciresinol dehydrogenase

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Acknowledgments

This work was, in part, supported by the Plant Factory Project of the Ministry of Economy, Technology, and Industry of Japan.

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Authors and Affiliations

  1. Bioorganic Research Institute, Suntory Foundation for Life Sciences, 8-1-1, Seikadai, 619-0284, Seika, Kyoto, Japan

    Honoo Satake, Tomotsugu Koyama, Erika Matsumoto, Kinuyo Morimoto & Jun Murata

  2. Research Institute, Suntory Global Innovation Center (SIC) Ltd., 619-0284, Seika, Kyoto, Japan

    Eiichiro Ono

Authors
  1. Honoo Satake
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  2. Tomotsugu Koyama
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  3. Erika Matsumoto
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  4. Kinuyo Morimoto
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  5. Eiichiro Ono
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  6. Jun Murata
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Correspondence to Honoo Satake , Tomotsugu Koyama , Erika Matsumoto , Kinuyo Morimoto , Eiichiro Ono or Jun Murata .

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  1. Department of Botany, Centre of Advanced Study, University of Calcutta, Kolkata, India

    Sumita Jha

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Satake, H., Koyama, T., Matsumoto, E., Morimoto, K., Ono, E., Murata, J. (2017). Metabolic Engineering of Lignan Biosynthesis Pathways for the Production of Transgenic Plant-Based Foods. In: Jha, S. (eds) Transgenesis and Secondary Metabolism. Reference Series in Phytochemistry. Springer, Cham. https://doi.org/10.1007/978-3-319-28669-3_11

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