Omega-3 Fatty Acid Content of Intact Muscle of Farmed Atlantic Salmon (Salmo salar) Examined by 1H MAS NMR Spectroscopy
In the present study, 1H MAS NMR spectra of intact salmon muscle was obtained and the total n-3 fatty acid content was quantified. The data from the MAS NMR analyses were compared to those obtained by estimating the n-3 fatty acid content in extracts from equivalent muscle samples (by 1H NMR and by gas chromatography (GC)). The results from MAS NMR and from 1H NMR were usually in good agreement, while the content of n-3 estimated by GC was in general higher than estimated by the NMR techniques.
HR MAS NMR is a rapid, noninvasive, and nondestructive method, with an experimental time of about 1–2 min. This opens up possibilities to examine the n-3 fatty acids in fish and fish products without any chemical pretreatment of the products, making NMR a valuable tool in the nutritional evaluation and general quality control of fresh and processed fish products.
KeywordsAtlantic salmon Fish fatty acid Omega-3 Marine lipids Quality control Nondestructive methods HR-MAS NMR
- 2.Drevon CD, Nenseter MS, Brude IR, Finstad HS, Kolset SO, Rustad AC. Omega-3 fatty acids- nutritional aspects. Can J Cardiol. 1995;11:47G–54G.Google Scholar
- 6.Aursand M, Jørgensen L, Grasdalen H. The use of high resolution 1H and 13C nuclear magnetic resonance (NMR) spectra to obtain information on the composition of fats and oils. Lipidforum. 1995;49:14–7.Google Scholar
- 11.Grasdalen H, Aursand M, Jørgensen L. NMR study of lipid fluidity in frozen red muscle of Atlantic salmon (Salmo salar): relation to autoxidation of lipids? In: Belton PS, Delgadillo I, Gil AM, Webb GA, editors. Proceedings of 2nd international conference on the application of magnetic resonance in food science. RSC Publishing; Cambridge, (Great Britain) 1995. p. 206–15.Google Scholar
- 16.Garroway AN. Magic-angle spinning of liquids. J Magn Reson. 1982;49:168–71.Google Scholar
- 17.Rutar V, Kovac M, Lahajnar G. Improved NMR spectra of liquid components in heterogenous samples. J Magn Reson. 1988;80:133–8.Google Scholar
- 18.Aursand M, Mabon F, Martin GJ. High-resolution 1H and 2H NMR spectroscopy of pure essential fatty acids for plants and animals. J Magn Res Anal. 1997;35:S91–S100.Google Scholar
- 22.Bankefors J, Kaszowska M, Schlechtriem C, Pickova J, Brännäs E, Edebo L, Kiessling A, Sandström C. A comparison of the metabolic profile on intact tissue and extracts of muscle and liver of juvenile Atlantic salmon (Salmo salar L.) – application to a short feeding study. Food Chem. 2011;129:1397–405.CrossRefGoogle Scholar