Omega-3 Fatty Acid Content of Intact Muscle of Farmed Atlantic Salmon (Salmo salar) Examined by 1H MAS NMR Spectroscopy

  • Marit Aursand
  • Inger B. Standal
  • I. S. Gribbestad
  • Iciar Martinez
Reference work entry


In the present study, 1H MAS NMR spectra of intact salmon muscle was obtained and the total n-3 fatty acid content was quantified. The data from the MAS NMR analyses were compared to those obtained by estimating the n-3 fatty acid content in extracts from equivalent muscle samples (by 1H NMR and by gas chromatography (GC)). The results from MAS NMR and from 1H NMR were usually in good agreement, while the content of n-3 estimated by GC was in general higher than estimated by the NMR techniques.

HR MAS NMR is a rapid, noninvasive, and nondestructive method, with an experimental time of about 1–2 min. This opens up possibilities to examine the n-3 fatty acids in fish and fish products without any chemical pretreatment of the products, making NMR a valuable tool in the nutritional evaluation and general quality control of fresh and processed fish products.


Atlantic salmon Fish fatty acid Omega-3 Marine lipids Quality control Nondestructive methods HR-MAS NMR 


  1. 1.
    Lindgren FT, Adamson GL, Shore VG, Nelson GJ, Schmidt PC. Effect of a salmon diet on the distribution of plasma lipoproteins and apolipoproteins in normolipidemic adult men. Lipids. 1991;26:97–101.CrossRefGoogle Scholar
  2. 2.
    Drevon CD, Nenseter MS, Brude IR, Finstad HS, Kolset SO, Rustad AC. Omega-3 fatty acids- nutritional aspects. Can J Cardiol. 1995;11:47G–54G.Google Scholar
  3. 3.
    Sprague M, Dick JR, Tocher DR. Impact of sustainable feeds on omega-3 long-chain fatty acid levels in farmed Atlantic salmon, 2006-2015. Sci Rep. 2016;6:21892.CrossRefGoogle Scholar
  4. 4.
    Aursand M, Bleivik B, Rainuzzo JR, Jørgensen L, Mohr V. Lipid distribution and composition of commerically farmed Atlantic salmon (Salmo salar). J Sci Food Agric. 1994;64:239–48.CrossRefGoogle Scholar
  5. 5.
    Aursand M, Jørgensen L, Grasdalen H. Quantitative high-resolution 13C nuclear magnetic resonance of anserine and lactate in white muscle of Atlantic salmon (Salmo Salar). Comp Biochem Physiol. 1995a;112B:315–21.CrossRefGoogle Scholar
  6. 6.
    Aursand M, Jørgensen L, Grasdalen H. The use of high resolution 1H and 13C nuclear magnetic resonance (NMR) spectra to obtain information on the composition of fats and oils. Lipidforum. 1995;49:14–7.Google Scholar
  7. 7.
    Aursand M, Jørgensen L, Grasdalen H. Positional distribution of w3 fatty acids in marine lipid triacylglycerols by high-resolution 13C Nuclear magnetic resonance spectroscopy. J Am Oil Chem Soc. 1995b;72:293–7.CrossRefGoogle Scholar
  8. 8.
    Aursand M, Rainuzzo JR, Grasdalen H. Quantitative high-resolution 13C and 1H nuclear magnetic resonance of w3 fatty acids from white muscle of Atlantic salmon (Salmo salar). J Am Oil Chem Soc. 1994;70:971–81.CrossRefGoogle Scholar
  9. 9.
    Gribbestad IS, Aursand M, Martinez I. High resolution 1H magnetic resonance spectroscopy of whole fish, fillets and extracts of farmed Atlantic salmon (Salmo salar) for quality assessment and compositional analyses. Aquaculture. 2005;250:445–57.CrossRefGoogle Scholar
  10. 10.
    Igarashi T, Aursand M, Hirata Y, Gribbestad IS, Wada S, Nonaka M. Nondestructive quantitative determination of docosahexaenoic acid and n-3 fatty acids in fish oils by high-resolution 1H nuclear magnetic resonance spectroscopy. J Am Oil Chem Soc. 2000;77:737–48.CrossRefGoogle Scholar
  11. 11.
    Grasdalen H, Aursand M, Jørgensen L. NMR study of lipid fluidity in frozen red muscle of Atlantic salmon (Salmo salar): relation to autoxidation of lipids? In: Belton PS, Delgadillo I, Gil AM, Webb GA, editors. Proceedings of 2nd international conference on the application of magnetic resonance in food science. RSC Publishing; Cambridge, (Great Britain) 1995. p. 206–15.Google Scholar
  12. 12.
    Ni QC, Eads TM. Liquid-phase composition of intact fruit tissue measured by high-resoution proton NMR. J Agric Food Chem. 1993;41:1026–34.CrossRefGoogle Scholar
  13. 13.
    Ni QC, Eads TM. Analysis by proton NMR of changes in liquid-phase and solid-phase components during ripening of banana. J Agric Food Chem. 1993;41:1035–40.CrossRefGoogle Scholar
  14. 14.
    Bligh EG, Dyer W. A rapid method to total lipid extraction and purification. J Can Biochem Physiol. 1959;37:911–7.CrossRefGoogle Scholar
  15. 15.
    Metcalfe LD, Schimtz AA, Pelka JR. Rapid preparation of fatty acid esters from lipids for gas chromatographic analysis. Anal Chem. 1966;38:514–5.CrossRefGoogle Scholar
  16. 16.
    Garroway AN. Magic-angle spinning of liquids. J Magn Reson. 1982;49:168–71.Google Scholar
  17. 17.
    Rutar V, Kovac M, Lahajnar G. Improved NMR spectra of liquid components in heterogenous samples. J Magn Reson. 1988;80:133–8.Google Scholar
  18. 18.
    Aursand M, Mabon F, Martin GJ. High-resolution 1H and 2H NMR spectroscopy of pure essential fatty acids for plants and animals. J Magn Res Anal. 1997;35:S91–S100.Google Scholar
  19. 19.
    Sacchi R, Medina I, Aubourg SP, Addeo F, Paolillo L. Proton nuclear-magnetic-resonance rapid and structure-specific determination of omega-3 polyunsaturated fatty-acids in fish lipids. J Am Oil Chem Soc. 1993;70:225–8.CrossRefGoogle Scholar
  20. 20.
    Castejon D, Villa P, Calvo MM, Santa-María G, Herraiz M, Herrera A. 1H-HRMAS NMR study of smoked Atlantic salmon. Magn Reson Chem. 2010;48:693–703.CrossRefGoogle Scholar
  21. 21.
    Nestor G, Bankefors J, Schlechtriem C, Brännäs E, Pickova J, Sandstrøm C. High-resolution 1H magic angle spinning NMR spectroscopy of intact Arctic charr (Salvelinus alpinus) muscle. Quantitative analysis of n-3 fatty acids, EPA and DPA. J Agric Food Chem. 2010;58:10799–803.CrossRefGoogle Scholar
  22. 22.
    Bankefors J, Kaszowska M, Schlechtriem C, Pickova J, Brännäs E, Edebo L, Kiessling A, Sandström C. A comparison of the metabolic profile on intact tissue and extracts of muscle and liver of juvenile Atlantic salmon (Salmo salar L.) – application to a short feeding study. Food Chem. 2011;129:1397–405.CrossRefGoogle Scholar

Copyright information

© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  • Marit Aursand
    • 6
  • Inger B. Standal
    • 1
  • I. S. Gribbestad
    • 2
  • Iciar Martinez
    • 3
    • 4
    • 5
  1. 1.Department of Processing TechnologySINTEF OceanTrondheimNorway
  2. 2.Cancer ClinicSt. Olav University HospitalTrondheimNorway
  3. 3.Plentzia Marine Research StationUniversity of the Basque Country UPV-EHUGorlizSpain
  4. 4.IKERBASQUEBasque Foundation for ScienceBilbaoSpain
  5. 5.Norwegian College of Fishery ScienceUniversity of TromsøTromsøNorway
  6. 6.Department of biotechnology and food scienceSINTEF Ocean Processing technology groupTrondheimNorway

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