High-Resolution NMR as Tool to Study Enzyme-Catalyzed Production of Fatty Acid Ethyl Esters from Marine Oils

  • Inger B. Standal
  • Ana K. Carvajal
  • Revilija Mozuraityte
  • Ivar Storrø
  • Trond Størseth
  • Elham Abbasi
  • Marit Aursand
Reference work entry


In this work, 1H and 13C NMR methods have been used to study the enzyme-catalyzed production of fatty acid ethyl esters from herring and seal oils, respectively. The reaction progress and the regiospecificity of the lipase from Candida antarctica (Novozym 435) have been studied. The fact that NMR differentiates acylglycerol isomers such as 1-MAG vs. 2-MAG and 1,2-DAG vs. 1,3-DAG, and different ethyl esters, (DHA, EPA vs. others ethyl esters) were essential in the evaluation of the regiospecificity and fatty acid-selectivity of the lipase. High-resolution NMR is shown to be a valuable tool to study process kinetics and in the optimization of enzymatic processes.


NMR Marine oils Enzymatic modification 


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Copyright information

© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  • Inger B. Standal
    • 1
  • Ana K. Carvajal
    • 2
  • Revilija Mozuraityte
    • 4
  • Ivar Storrø
    • 2
  • Trond Størseth
    • 2
  • Elham Abbasi
    • 3
  • Marit Aursand
    • 5
  1. 1.Department of Processing TechnologySINTEF OceanTrondheimNorway
  2. 2.SINTEF OceanTrondheimNorway
  3. 3.Department of BiotechnologyNorwegian University of Natural Science and TechnologyTrondheimNorway
  4. 4.Processing technologySINTEF Fisheries and AquacultureTrondheimNorway
  5. 5.Department of biotechnology and food scienceSINTEF Ocean Processing technology groupTrondheimNorway

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