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Modern Magnetic Resonance pp 1519–1534Cite as

In Situ Studies of Plant Seeds Using 13C or 1H MAS NMR and 1H PFG NMR Approaches

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Abstract

NMR can be used for in situ studies of lipidic components, mainly triacylglycerols (TAG), in mature seeds. Lipids can be one of the most abundant constituents of seeds in oleaginous crops. The inputs of high-resolution solid-state 1H or 13C NMR and pulsed field gradient 1H NMR (PFG NMR) are presented in this chapter. By combining single pulse excitation (SP) and cross-polarization (CP) experiments, both liquid and solid domains of plant seeds can be characterized and the assignments of the corresponding 13C and 1H can be performed. As TAG are confined in oil bodies (OBs), analysis of their diffusion properties, determined with PFG NMR, is a well-suited experimental approach to determine OB sizes. In fact, at long diffusion time, TAG mean squared displacement is limited by the OB size. In order to access the OB size distribution, strong intensities of magnetic field gradients are generally required. However, using a standard liquid-phase NMR probe equipped with a weak-intensity gradient coil, the mean size of OBs can be determined. OB diameters obtained by PFG NMR were fully consistent with previously published values obtained by microscopy techniques.

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Author information

Authors and Affiliations

  1. INAC-MEM, LRM, Univ. Grenoble Alpes, F-38000, Grenoble, France

    Marina Gromova, Pierre-Alain Bayle & Michel Bardet

  2. CEA, INAC, MEM, LRM, F-38000, Grenoble, France

    Marina Gromova, Pierre-Alain Bayle & Michel Bardet

  3. INAC-SPrAM, Univ. Grenoble Alpes, F-38000, Grenoble, France

    Armel Guillermo

  4. INAC-SPrAM, CNRS, F-38000, Grenoble, France

    Armel Guillermo

  5. INAC-SPrAM, CEA, F-38000, Grenoble, France

    Armel Guillermo

Authors
  1. Marina Gromova
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  2. Armel Guillermo
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  3. Pierre-Alain Bayle
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  4. Michel Bardet
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Corresponding author

Correspondence to Michel Bardet .

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Editors and Affiliations

  1. Royal Society of Chemistry, London, United Kingdom

    Graham A. Webb

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Gromova, M., Guillermo, A., Bayle, PA., Bardet, M. (2018). In Situ Studies of Plant Seeds Using 13C or 1H MAS NMR and 1H PFG NMR Approaches. In: Webb, G. (eds) Modern Magnetic Resonance. Springer, Cham. https://doi.org/10.1007/978-3-319-28388-3_18

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