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Solid-State 2H NMR Studies of Water-Mediated Lipid Membrane Deformation

  • Trivikram R. Molugu
  • Xiaolin Xu
  • Soohyun Lee
  • K. J. Mallikarjunaiah
  • Michael F. Brown
Reference work entry

Abstract

The application of solid-state 2H nuclear magnetic resonance (NMR) spectroscopy gives a powerful approach for investigating hydration-mediated effects on lipid bilayer structure and dynamics. The extent to which lipid bilayers are deformed by dehydration stress is inherent to understanding how lipid-protein interactions affect biomembrane functioning. For liquid-crystalline membranes, the average structure is manifested by the segmental order parameters (SCD) of the lipids. Structural quantities, such as the area per lipid and volumetric bilayer thickness, are obtained by a mean-torque analysis of 2H NMR order parameters. Removal of water in the liquid-crystalline state gives a reduction of the mean area per lipid, together with a corresponding increase in volumetric bilayer thickness. Measurements of order parameters versus osmotic pressure yield the elastic area compressibility modulus and the corresponding bilayer thickness at an atomistic level. Furthermore, solid-state 2H NMR relaxation rates of lipid bilayers at varying hydration levels afford new insights into the role of water in membrane structural dynamics and viscoelastic properties. Model-free interpretation of spin-lattice (R1Z) and transverse (\( {R}_2^{\mathrm{QE}} \)) relaxation rates suggests that collective chain motions described as order-director fluctuations dominantly contribute to the relaxation. In a continuum picture, elastic deformations in such materials are collective hydrodynamic phenomena with motional time scales spanning many decades (picoseconds to seconds). The dynamic processes mainly affecting the spin‐spin relaxation have characteristic time scales much longer than those contributing to spin‐lattice relaxation. Such studies probe membrane interactions involving collective bilayer undulations, order-director fluctuations, and lipid molecular protrusions, giving a unique source of information about intermolecular forces pertinent to biomembrane structure and function.

Keywords

Lipid bilayers Liquid crystals Membranes deformation Membrane elasticity Molecular dynamics NMR relaxation NMR spectroscopy Order-director fluctuations Osmotic stress 

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Copyright information

© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  • Trivikram R. Molugu
    • 1
  • Xiaolin Xu
    • 2
  • Soohyun Lee
    • 1
  • K. J. Mallikarjunaiah
    • 3
  • Michael F. Brown
    • 4
  1. 1.Department of Chemistry and BiochemistryUniversity of ArizonaTucsonUSA
  2. 2.Department of PhysicsUniversity of ArizonaTucsonUSA
  3. 3.Department of PhysicsIndian Institute of ScienceBangaloreIndia
  4. 4.Department of Chemistry and Biochemistry, and Department of PhysicsUniversity of ArizonaTucsonUSA

Section editors and affiliations

  • G. E. Martin
    • 1
  • Hazime Saito
    • 2
    • 3
  • Yining Huang
    • 4
  1. 1.Process & Analytical Chemistry, NMR Structure ElucidationMerck Research LaboratoriesRahwayUnited States
  2. 2.203, 3-15-21 Sumiyoshi-honmachiKobeJapan
  3. 3.Himeji Institute of Technology, University of HyogoKamigoriJapan
  4. 4.Department of Chemistry,The University of Western OntarioOntarioCanada

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