Abstract
Electroporation is a phenomenon that occurs in the membranes of biological cells exposed to strong electric fields. The exposure of cells to strong electric fields leads to temporary permeabilization of biological cells, thus allowing easier transport of ions and molecules across the cell membrane. Electroporation is already widely used in several biomedical and biotechnological applications. The efficacy of electroporation strongly depends on the electric field parameters and also other biophysical parameters. Strong electric fields are generated between the electrodes by the delivery of high-voltage electric pulses to the electrodes close to each other. Results obtained in different electroporation studies may vary significantly. This may be because the determination and description of the electric fields to which cells are exposed during experimental work were not adequately addressed or at least were not reported in sufficient details. To enable the reproduction of electroporation experiments, researchers should adequately measure the electroporation pulses, evaluate the generated electric field, and provide adequate description of experimental methods. In this chapter, parametrization and parameters of electroporation pulses are described, techniques of controlling the generation sequence of electroporation pulses are specified, and recommendations for electroporation pulse measurement and reporting in electroporation studies are suggested.
References
Arena CB, Sano MB, Rossmeisl JH Jr, Caldwell JL, Garcia PA, Rylander MN, Davalos RV (2011) High-frequency irreversible electroporation (H-FIRE) for non-thermal ablation without muscle contraction. Biomed Eng Online 10:102
Arnaud-Cormos D, Leveque P, Wu YH, Sanders JM, Gundersen MA, Vernier TP (2011) Microchamber setup characterization for nanosecond pulsed electric field exposure. IEEE T Biomed Eng 58:1656â1662
Batista Napotnik T, ReberÅ¡ek M, Vernier PT, Mali B, MiklavÄiÄ D (2016) Effects of high voltage nanosecond electric pulses on eukaryotic cells (in vitro): a systematic review. Bioelectrochemistry 110:1â12
Campana LG, Clover AJP, Valpione S, Quaglino P, Gehl J, Kunte C, Snoj M, ÄemaÅŸar M, Rossi CR, MiklavÄiÄ D, SerÅ¡a G (2016) Recommendations for improving the quality of reporting clinical electrochemotherapy studies based on qualitative systematic review. Radiol Oncol 50:1â13
ÄoroviÄ S, Pavlin M, MiklavÄiÄ D (2007) Analytical and numerical quantification and comparison of the local electric field in the tissue for different electrode configurations. Biomed Eng Online 6:37
Cukjati D, Batiuskaite D, André F, MiklavÄiÄ D, Mir LM (2007) Real time electroporation control for accurate and safe in vivo non-viral gene therapy. Bioelectrochemistry 70:501â507
Guo S, Jackson DL, Burcus NI, Chen YJ, Xiao S, Heller R (2014) Gene electrotransfer enhanced by nanosecond pulsed electric fields. Mol Ther Methods Clin Dev 1:14043
Haberl S, MiklavÄiÄ D, SerÅ¡a G, Frey W, Rubinsky B (2013) Cell membrane electroporation â part 2: the applications. IEEE Electr Insul M 29:29â37
Kenaan M, El Amari S, Silve A, Merla C, Mir LM, Couderc V, Arnaud-Cormos D, Leveque P (2011) Characterization of a 50-Ω exposure setup for high-voltage nanosecond pulsed electric field bioexperiments. IEEE T Biomed Eng 58:207â214
Kolb JF (2010) Generation of ultrashort pulse. In: Pakhomov AG, MiklavÄiÄ D, Markov MS (eds) Advanced electroporation techniques in biology in medicine. CRC Press, Boca Raton, pp 341â352
Kolb JF, Kono S, Schoenbach KH (2006) Nanosecond pulsed electric field generators for the study of subcellular effects. Bioelectromagnetics 27:172â187
Kotnik T, MiklavÄiÄ D (2006) Theoretical evaluation of voltage inducement on internal membranes of biological cells exposed to electric fields. Biophys J 90:480â491
Kotnik T, MiklavÄiÄ D, Mir LM (2001) Cell membrane electropermeabilization by symmetrical bipolar rectangular pulses. Part II. Reduced electrolytic contamination. Bioelectrochemistry 54:91â95
Kotnik T, Pucihar G, ReberÅ¡ek M, Mir LM, MiklavÄiÄ D (2003) Role of pulse shape in cell membrane electropermeabilization. Biochim Biophys Acta 1614:193â200
Kotnik T, Kramar P, Pucihar G, MiklavÄiÄ D, Tarek M (2012) Cell membrane electroporation â part 1: the phenomenon. IEEE Electr Insul M 28:14â23
Pakhomov AG, Semenov I, Xiao S, Pakhomova ON, Gregory B, Schoenbach KH, Ullery JC, Beier HT, Rajulapati SR, Ibey BL (2014) Cancellation of cellular responses to nanoelectroporation by reversing the stimulus polarity. Cell Mol Life Sci 71:4431â4441
Pakhomova ON, Gregory BW, Khorokhorina VA, Bowman AM, Xiao S, Pakhomov AG (2011) Electroporation-induced electrosensitization. PLoS One 6:e17100
Pucihar G, Krmelj J, ReberÅ¡ek M, Batista Napotnik T, MiklavÄiÄ D (2011) Equivalent pulse parameters for electroporation. IEEE T Biomed Eng 58:3279â3288
Raso J, Frey W, Ferrari G, Pataro G, Knorr D, Teissie J, MiklavÄiÄ D (2016) Recommendations guidelines on the key information to be reported in studies of application of PEF technology in food and biotechnological processes. Innovative Food Sci Emerg Technol. doi:10.1016/j.ifset.2016.08.003
ReberÅ¡ek M, Faurie C, KanduÅ¡er M, ÄoroviÄ S, Teissié J, Rols MP, MiklavÄiÄ D (2007) Electroporator with automatic change of electric field direction improves gene electrotransfer in vitro. Biomed Eng Online 6:25
ReberÅ¡ek M, MiklavÄiÄ D, Bertacchini C, Sack M (2014) Cell membrane electroporation â part 3: the equipment. IEEE Electr Insul M 30:8â18
Rems L, UÅ¡aj M, KanduÅ¡er M, ReberÅ¡ek M, MiklavÄiÄ D, Pucihar G (2013) Cell electrofusion using nanosecond electric pulses. Sci Rep 3:3382
Å atkauskas S, Bureau MF, Puc M, Mahfoudi A, Scherman D, MiklavÄiÄ D, Mir LM (2002) Mechanisms of in vivo DNA electrotransfer: respective contributions of cell electropermeabilization and DNA electrophoresis. Mol Ther 5:133â140
Smith PW (2002) Transient electronics: pulsed circuit technology. Wiley, New York
Åœgalin MK, HodÅŸiÄ D, ReberÅ¡ek M, KanduÅ¡er M (2012) Combination of microsecond and nanosecond pulsed electric field treatments for inactivation of Escherichia coli in water samples. J Membr Biol 245:643â650
ÅœupaniÄ A, RibariÄ S, MiklavÄiÄ D (2007) Increasing the repetition frequency of electric pulse delivery reduces unpleasant sensations that occur in electrochemotherapy. Neoplasma 54:246â250
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Reberšek, M. (2017). Beyond Electroporation Pulse Parameters: From Application to Evaluation. In: Miklavcic, D. (eds) Handbook of Electroporation. Springer, Cham. https://doi.org/10.1007/978-3-319-26779-1_222-1
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DOI: https://doi.org/10.1007/978-3-319-26779-1_222-1
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