Abstract
Pullulan is a microbial exopolysaccharide produced by polymorphic fungus Aureobasidium pullulans. Owing to its structure, consisting of α(1→4) and α(1→6) linkages, it has unique properties and hence is suitable for a variety of commercial applications in various field including food, pharmaceuticals, and biomedical. In this chapter, biosynthesis, production through microbial fermentation, and influence of fermentation conditions like pH, temperature, aeration, agitation on microbial growth, pullulan yield, pullulan purity, and molecular weight are discussed. Later, options of using various agricultural wastes as carbon source for pullulan production are considered.
Keywords
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- ATP:
-
Adenosine triphosphate
- DE:
-
Dextrose equivalent. It is a measure of reducing sugars present in starch hydrolysate. Carbohydrate molecules like starch are polymers of monosaccharide. The extent of polymerization is indirectly measured by DE. In starch hydrolysate DE represents the amount of reducing sugar as dextrose. Dextrose equivalent of starch is 100 and that of glucose/dextrose is 100. Starch hydrolysates with DE ranging between 1 and 13 are termed as dextrins and those with DE in the range of 13–20 are called maltodextrins. DE for glucose syrup is typically greater than 20, and commercial glucose syrup has a DE of 40–42.
- UDPG:
-
Uridine diphosphate glucose
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Ponnusami, V., Gunasekar, V. (2015). Production of Pullulan by Microbial Fermentation. In: Ramawat, K., Mérillon, JM. (eds) Polysaccharides. Springer, Cham. https://doi.org/10.1007/978-3-319-16298-0_58
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DOI: https://doi.org/10.1007/978-3-319-16298-0_58
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