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Characterization and Imaging of Solid Lipid Nanoparticles and Nanostructured Lipid Carriers

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Handbook of Nanoparticles

Abstract

Solid lipid nanoparticles (SLNs) and nanostructured lipid carriers (NLCs), the second generation of SLNs, are colloidal drug carrier systems that can be used for controlled drug delivery via various administration routes. They introduce various advantages over traditional dosage forms and their colloidal counterparts. SLN and NLC products are available in the market of the European community, and lipid nanotechnology has been increasingly attracted by the industry. Moreover, studies on lipid nanoparticles have been focused on targeting of drugs to the specific sites of the body, thus surface modification and treatment of SLN and NLC in the last decades. Naturally, several parameters must be taken into consideration for design of well-performing formulations, which have a long-term stability. A lot of analytical methods, which give scientists extensive informations, are essential for characterization of SLN and NLC. Investigation of factors affecting their physicochemical properties and common techniques used for their characterization will be introduced in this chapter. Determination methods of particle size, particle charge, and surface characteristics, crystallographic and structural investigations, and imaging of SLN and NLC will be discussed. In vivo and in vitro experiments will also be summarized to describe future direction of researches.

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References

  1. R. Abbasalipourkabir, A. Salehzadeh, R. Abdullah, Characterization and stability of nanostructured lipid carriers as drug delivery system. Pak. J. Biol. Sci. 15, 141–146 (2012)

    Article  Google Scholar 

  2. M.A. Iqbal, S. Md, J.K. Sahni et al., Nanostructured lipid carriers system: recent advances in drug delivery. J. Drug Target. 20, 813–830 (2012)

    Article  Google Scholar 

  3. W. Mehnert, K. Mäder, Solid lipid nanoparticles: production, characterization and applications. Adv. Drug Deliv. Rev. 64, 83–101 (2012)

    Article  Google Scholar 

  4. K.K. Sawant, S.S. Dodiya, Recent advances and patents on solid lipid nanoparticles. Recent Pat. Drug Deliv. Formul. 2, 120–135 (2008)

    Article  Google Scholar 

  5. B.D. Patel, R.V. Modi, N.A. Thakkar et al., Development and characterization of solid lipid nanoparticles for enhancement of oral bioavailability of raloxifene. J. Pharm. Bioallied Sci. 4(Suppl 1), S14–S16 (2012)

    Google Scholar 

  6. K. Hippalgaonkar, G.R. Adelli, K. Hippalgaonkar et al., Indomethacin-loaded solid lipid nanoparticles for ocular delivery: development, characterization, and in vitro evaluation. J. Ocul. Pharmacol. Ther. 29, 216–228 (2013)

    Article  Google Scholar 

  7. A.S. Joshi, H.S. Patel, V.S. Belgamwar et al., Solid lipid nanoparticles of ondansetron HCl for intranasal delivery: development, optimization and evaluation. J. Mater. Sci. Mater. Med. 23, 2163–2175 (2012)

    Article  Google Scholar 

  8. W. Wang, R. Zhu, Q. Xie et al., Enhanced bioavailability and efficiency of curcumin for the treatment of asthma by its formulation in solid lipid nanoparticles. Int. J. Nanomed. 7, 3667–3677 (2012)

    Article  Google Scholar 

  9. G. Niculae, I. Lacatusu, N. Badea et al., Lipid nanoparticles based on butyl-methoxydibenzoylmethane: in vitro UVA blocking effect. Nanotechnology 23, 315704 (2012)

    Article  Google Scholar 

  10. G. Shiva, M. Somaye, J.M. Reza, Improved photostability, reduced skin permeation and irritation of isotretinoin by solid lipid nanoparticles. Acta Pharm. 62, 547–562 (2012)

    Article  Google Scholar 

  11. T. Jie, X. Pang, Y. Kefu et al., Preparation, characterization and in vivo distribution of solid lipid nanoparticles loaded with cisplatin. Pharmazie 8, 593–597 (2008)

    Google Scholar 

  12. N. Chattopadhyay, J. Zastre, H.L. Wong et al., Solid lipid nanoparticles enhance the delivery of the HIV protease inhibitor, atazanavir, by a human brain endothelial cell line. Pharm. Res. 25, 2262–2271 (2008)

    Article  Google Scholar 

  13. L. Reddy, R. Sharma, K. Chuttani et al., Etoposide-incorporated tripalmitin nanoparticles with different surface charge: formulation, characterization, radiolabeling, and biodistribution studies. AAPS J. 6, 55–64 (2004)

    Article  Google Scholar 

  14. Y.H. Yu, E. Kim, D.E. Park et al., Cationic solid lipid nanoparticles for co-delivery of paclitaxel and siRNA. Eur. J. Pharm. Biopharm. 80, 268–273 (2012)

    Article  Google Scholar 

  15. R.H. Müller, K. Mäder, S. Gohla, Solid lipid nanoparticles (SLN) for controlled drug delivery-a review of the state of the art. Eur. J. Pharm. Biopharm. 50, 161–177 (2000)

    Article  Google Scholar 

  16. J. Yun, S. Zhang, S. Shen et al., Continuous production of solid lipid nanoparticles by liquid flow-focusing and gas displacing method in microchannels. Chem. Eng. Sci. 64, 4115–4122 (2009)

    Article  Google Scholar 

  17. N.A. Al-Haj, R. Abdullah, S. Ibrahim et al., Tamoxifen drug loading solid lipid nanoparticles prepared by hot high pressure homogenization techniques. Am. J. Pharmacol. Toxicol. 3, 219–224 (2008)

    Article  Google Scholar 

  18. C. Charcosset, A. El-Harati, H. Fessi, Preparation of solid lipid nanoparticles using a membrane contactor. J. Control. Release 108, 112–120 (2005)

    Article  Google Scholar 

  19. A.R.S. De Sousa, A.L. Simplício, H.C. De Sousa et al., Preparation of glyceryl monostearate-based particles by PGSS®-Application to caffeine. J. Supercrit. Fluids 43, 120–125 (2007)

    Article  Google Scholar 

  20. S. Salmaso, N. Elvassore, A. Bertucco et al., Production of solid lipid submicron particles for protein delivery using a novel supercritical gas-assisted melting atomization process. J. Pharm. Sci. 98, 640–650 (2009)

    Article  Google Scholar 

  21. D.T. Baviskar, A.S. Amritkar, H.S. Chaudhari et al., Modulation of drug release from nanocarriers loaded with a poorly water soluble drug (flurbiprofen) comprising natural waxes. Pharmazie 67, 701–705 (2012)

    Google Scholar 

  22. M. Joshi, V. Patravale, Nanostructured lipid carrier (NLC) based gel of celecoxib. Int. J. Pharm. 346, 124–132 (2008)

    Article  Google Scholar 

  23. A.E. Yassin, M.K. Anwer, H.A. Mowafy et al., Optimization of 5-flurouracil solid-lipid nanoparticles: a preliminary study to treat colon cancer. Int. J. Med. Sci. 7, 398–408 (2010)

    Article  Google Scholar 

  24. S. Li, B. Zhao, F. Wang et al., Yak interferon-alpha loaded solid lipid nanoparticles for controlled release. Res. Vet. Sci. 88, 148–153 (2010)

    Article  Google Scholar 

  25. X. Zhang, S. Lü, J. Han et al., Preparation, characterization and in vivo distribution of solid lipid nanoparticles loaded with syringopicroside. Pharmazie 66, 404–407 (2011)

    Google Scholar 

  26. A. Madhusudhan, G.B. Reddy, M. Venkatesham et al., Design and evaluation of efavirenz loaded solid lipid nanoparticles to improve the oral bioavailability. Int. J. Pharm. Pharm. Sci. Res. 2, 84–89 (2012)

    Google Scholar 

  27. C.B. Fernandes, S. Mandawgade, V.B. Patravale, Solid lipid nanoparticles of etoposide using solvent emulsification diffusion technique for parenteral administration. Int. J. Pharm. Biosci. Technol. 1, 27–33 (2013)

    Google Scholar 

  28. C.V. Pardeshi, P.V. Rajput, V.S. Belgamwar et al., Novel surface modified solid lipid nanoparticles as intranasal carriers for ropinirole hydrochloride: application of factorial design approach. Drug Deliv. 20, 47–56 (2013)

    Article  Google Scholar 

  29. A.R. Gardouh, S. Gad, H.M. Ghonaim et al., Design and characterization of glyceryl monostearate solid lipid nanoparticles prepared by high shear homogenization. Br. J. Pharm. Res. 3, 326–346 (2013)

    Article  Google Scholar 

  30. Y.S.R. Elnaggar, M.A. El-Massik, O.Y. Abdallah, Fabrication, appraisal, and transdermal permeation of sildenafil citrate-loaded nanostructured lipid carriers versus solid lipid nanoparticles. Int. J. Nanomed. 6, 3195–3205 (2011)

    Article  Google Scholar 

  31. B. Mohanty, D.K. Majumdar, S.K. Mishra et al., Development and characterization of itraconazole-loaded solid lipid nanoparticles for ocular delivery. Pharm. Dev. Technol 20, 458–464 (2015)

    Google Scholar 

  32. E. Lasoń, E. Sikora, J. Ogonowski, Influence of process parameters on properties of nanostructured lipid carriers (NLC) formulation. Acta Biochim. Pol. 60, 773–777 (2013)

    Google Scholar 

  33. S.S. Shidhaye, R. Vaidya, S. Sutar et al., Solid lipid nanoparticles and nanostructured lipid carriers-innovative generations of solid lipid carriers. Curr. Drug Deliv. 5, 324–331 (2008)

    Article  Google Scholar 

  34. F. Wan, J. You, Y. Sun et al., Studies on PEG-modified SLNs loading vinorelbine bitartrate (I): preparation and evaluation in vitro. Int. J. Pharm. 359, 104–110 (2008)

    Article  Google Scholar 

  35. K. Manjunath, V. Venkateswarlu, A. Hussain, Preparation and characterization of nitrendipine solid lipid nanoparticles. Pharmazie 66, 178–186 (2011)

    Google Scholar 

  36. M. Üner, Preparation, characterization and physicochemical properties of solid lipid nanoparticles (SLN) and nanostructured lipid carriers (NLC): their benefits as colloidal drug carrier systems. Pharmazie 61, 375–386 (2006)

    Google Scholar 

  37. H. Bunjes, M.H.J. Koch, K. Westesen, Influence of emulsifiers on the crystallization of solid lipid nanoparticles. J. Pharm. Sci. 92, 1509–1520 (2003)

    Article  Google Scholar 

  38. A. Lippacher, R.H. Müller, K. Mäder, Semisolid SLNTM dispersions for topical application: influence of formulation and production parameters on viscoelastic properties. Eur. J. Pharm. Biopharm. 53, 155–160 (2002)

    Article  Google Scholar 

  39. A. Dingler, R.P. Blum, H. Niehus et al., Solid lipid nanoparticles (SLNTM/LipopearlsTM) – a pharmaceutical and cosmetic carrier for the application of vitamin E in dermal products. J. Microencapsul. 16, 751–767 (1999)

    Article  Google Scholar 

  40. W. Mehnert, K. Mäder, Solid lipid nanoparticles. Production, characterization and applications. Adv. Drug Deliv. Rev. 47, 165–196 (2001)

    Article  Google Scholar 

  41. V.B. Patravale, A.V. Ambarkhane, Study of solid lipid nanoparticles with respect to particle size distribution and drug loading. Pharmazie 58, 392–395 (2003)

    Google Scholar 

  42. C. Freitas, R.H. Müller, Spray-drying of solid lipid nanoparticles (SLNTM). Eur. J. Pharm. Biopharm. 46, 145–151 (1998)

    Article  Google Scholar 

  43. A. Califice, F. Michel, G. Dislaire et al., Influence of particle shape on size distribution measurements by 3D and 2D image analyses and laser diffraction. Powder Technol. 237, 67–75 (2013)

    Article  Google Scholar 

  44. R.H. Müller, W. Mehnert, Particle and Surface Characterisation Methods (Wissenschaftliche Verlagsgesellschaft, Stuttgart, 1997)

    Google Scholar 

  45. M. Üner, R.H. Müller, Sage extract entrapped in nanostructured lipid carriers for application into the mouth cavity for oral hygiene. Curr. Top. Nutraceutical Res. 10, 193–200 (2012)

    Google Scholar 

  46. M. Üner, Solid lipid nanoparticles and nanostructured lipid carriers for cancer therapy, in Handbook of Clinical Nanomedicine – From Bench to Bedside, ed. by R. Bawa, G.F. Audette, I. Rubinstein (Raj Bawa, Series Editor). Pan Stanford Series in Nanomedicine, vol. 1 (Pan Stanford Publishing, Singapore, 2015 in press)

    Google Scholar 

  47. http://nanocomposix.com

  48. C. Freitas, R.H. Müller, Effect of light and temperature on zeta potential and physical stability in solid lipid nanoparticle (SLNTM) dispersions. Int. J. Pharm. 168, 221–229 (1998)

    Article  Google Scholar 

  49. R.H. Müller, Zetapotential und Partikelladung-Kurze Theorie, praktische Mesdurchfuhrung, Dateninterpretation (Wissenschaftliche Verlagsgesellschaft mbH, Stuttgart, 1996)

    Google Scholar 

  50. R. Cavalli, O. Caputo, M.E. Carlotti et al., Sterilization and freeze-drying of drug-free and drug-loaded solid lipid nanoparticles. Int. J. Pharm. 148, 47–54 (1997)

    Article  Google Scholar 

  51. M. Üner, G. Yener, Importance of solid lipid nanoparticles (SLN) in various administration routes and future perspectives. Int. J. Nanomed. 2, 289–300 (2007)

    Google Scholar 

  52. X.-Y. Ying, D. Cui, L. Yu et al., Solid lipid nanoparticles modified with chitosan oligosaccharides for the controlled release of doxorubicin. Carbohydr. Polym. 84, 1357–1364 (2011)

    Article  Google Scholar 

  53. C. Carrillo, N. Sánchez-Hernández, E. García-Montoya et al., DNA delivery via cationic solid lipid nanoparticles (SLNs). Eur. J. Pharm. Sci. 49, 157–165 (2013)

    Article  Google Scholar 

  54. V. Klang, C. Valenta, N.B. Matsko, Electron microscopy of pharmaceutical systems. Micron 44, 45–74 (2013)

    Article  Google Scholar 

  55. R. Erni, M.D. Rossell, C. Kisielowski et al., Atomic-resolution imaging with a sub-50-pm electron probe. Phys. Rev. Lett. 102, 96–101 (2009)

    Article  Google Scholar 

  56. K. Jores, W. Mehnert, M. Drechsler et al., Investigations on the structure of solid lipid nanoparticles (SLN) and oil-loaded solid lipid nanoparticles by photon correlation spectroscopy, field-flow fractionation and transmission electron microscopy. J. Control. Release 95, 217–227 (2004)

    Article  Google Scholar 

  57. I. Lacatusu, N. Badea, A. Murariu et al., The encapsulation effect of UV molecular absorbers into biocompatible lipid nanoparticles. Nanoscale Res. Lett. 6, 73 (2011)

    Article  Google Scholar 

  58. A. Dubes, H. Parrot-Lopez, W. Abdelwahed et al., Scanning electron microscopy and atomic force microscopy imaging of solid lipid nanoparticles derived from amphiphilic cyclodextrins. Eur. J. Pharm. Biopharm. 55, 279–282 (2003)

    Article  Google Scholar 

  59. S. Vahabi, B.N. Salman, A. Javanmard, Atomic force microscopy application in biological research: a review study. Iran. J. Med. Sci. 38, 76–83 (2013)

    Google Scholar 

  60. H. Chen, X. Chang, D. Du et al., Podophyllotoxin-loaded solid lipid nanoparticles for epidermal targeting. J. Control. Release 110, 296–306 (2006)

    Article  Google Scholar 

  61. C. Olbrich, U. Bakowsky, C.M. Lehr et al., Cationic solid-lipid nanoparticles can efficiently bind and transfect plasmid DNA. J. Control. Release 77, 345–355 (2001)

    Article  Google Scholar 

  62. P. Shahgaldian, L. Quattrocchi, J. Gualbert et al., AFM imaging of calixarene based solid lipid nanoparticles in gel matrices. Eur. J. Pharm. Biopharm. 55, 107–113 (2003)

    Article  Google Scholar 

  63. P. Shahgaldian, E. Da Silva, A.W. Coleman et al., Para-acyl-calix-arene based solid lipid nanoparticles (SLNs): a detailed study of preparation and stability parameters. Int. J. Pharm. 253, 23–38 (2003)

    Article  Google Scholar 

  64. H. Bunjes, M.H.J. Koch, K. Westesen, Crystallization tendency and polymorphic transitions in triglyceride nanoparticles. Int. J. Pharm. 129, 159–173 (1996)

    Article  Google Scholar 

  65. J.S. Negi, P. Chattopadhyay, A.K. Sharma et al., Development and evaluation of glyceryl behenate based solid lipid nanoparticles (SLNs) using hot self-nanoemulsification (SNE) technique. Arch. Pharm. Res. 37, 361–370 (2014)

    Google Scholar 

  66. M. Üner, E.F. Karaman, Z. Aydoğmuş, Solid lipid nanoparticles and nanostructured lipid carriers of loratadine for topical application: physicochemical stability and drug penetration through rat skin. Trop. J. Pharm. Res. 13, 653–660 (2014)

    Google Scholar 

  67. K. Westesen, B. Siekmann, M.H.J. Koch, Investigations on the physical state of lipid nanoparticles by synchrotron radiation X-ray diffraction. Int. J. Pharm. 93, 189–199 (1993)

    Article  Google Scholar 

  68. R.H. Müller, W. Mehnert, J.S. Lucks et al., Solid lipid nanoparticles (SLN) – an alternative colloidal carrier system for controlled drug delivery. Eur. J. Pharm. Biopharm. 41, 62–69 (1995)

    Google Scholar 

  69. A. Zur Mühlen, C. Schwarz, W. Mehnert, Solid lipid nanoparticles (SLN) for controlled drug delivery-Drug release and release mechanism. Eur. J. Pharm. Biopharm. 45, 149–155 (1998)

    Article  Google Scholar 

  70. B. Siekmann, K. Westesen, Thermoanalysis of recrystallisation process of melt-homogenized glyceride nanoparticles. Colloids Surf. B: Biointerfaces 3, 159–175 (1994)

    Article  Google Scholar 

  71. V. Venkateswarlu, K. Manjunath, Preparation, characterization and in vitro release kinetics of clozapine solid lipid nanoparticles. J. Control. Release 95, 627–638 (2004)

    Article  Google Scholar 

  72. J.L. Ford, P. Timmins, Pharmaceutical Thermal Analysis (Ellis Horwood, Chichester, 1989)

    Google Scholar 

  73. C. Allais, G. Keller, P. Lesieur et al., X-ray diffraction/calorimetry coupling. A tool for polymorphism control. J. Therm. Anal. Calorim. 74, 723–728 (2003)

    Article  Google Scholar 

  74. A.I. Kitaigorodskii, Organic Chemical Crystallography (Springer, New York, 1984)

    Google Scholar 

  75. D.M. Small, Handbook of Lipid Research. The Physical Chemistry of Lipids (Plenum Press, New York, 1986)

    Google Scholar 

  76. C.W. How, R. Abdullah, R. Abbasalipourkabir, Physicochemical properties of nanostructured lipid carriers as colloidal carrier system stabilized with polysorbate 20 and polysorbate 80. Afr. J. Biotechnol. 10, 1684–1689 (2011)

    Google Scholar 

  77. K. Jores, W. Mehnert, K. Mäder, Physicochemical investigations on solid lipid nanoparticles and on oil-loaded solid lipid nanoparticles: a nuclear magnetic resonance and electron spin resonance study. Pharm. Res. 20, 1274–1283 (2003)

    Article  Google Scholar 

  78. B. Angelov, A. Angelova, R. Mutafchieva et al., SAXS investigation of a cubic to a sponge (L3) phase transition in self-assembled lipid nanocarriers. Phys. Chem. Chem. Phys. 13, 3073–3081 (2011)

    Article  Google Scholar 

  79. A. Illing, T. Unruh, M.H.J. Koch, Investigation on particle self-assembly in solid lipid-based colloidal drug carrier systems. Pharm. Res. 21, 592–597 (2004)

    Article  Google Scholar 

  80. C. Rümenapp, B. Gleich, A. Haase, Magnetic nanoparticles in magnetic resonance imaging and diagnostics. Pharm. Res. 29, 1165–1179 (2012)

    Article  Google Scholar 

  81. M.A. Schubert, M. Harms, C.C. Müller-Goymann, Structural investigations on lipid nanoparticles containing high amounts of lecithin. Eur. J. Pharm. Sci. 27, 226–236 (2006)

    Article  Google Scholar 

  82. E. Zimmermann, E.B. Souto, R.H. Müller, Physicochemical investigations on the structure of drug-free and drug-loaded solid lipid nanoparticles (SLN) by means of DSC and 1H NMR. Pharmazie 60, 508–513 (2005)

    Google Scholar 

  83. M.S. Berkman, Y. Yazan, Solid lipid nanoparticles: a possible vehicle for zinc oxide and octocrylene. Pharmazie 67, 202–208 (2012)

    Google Scholar 

  84. K. Jores, A. Haberland, S. Wartewig et al., Solid lipid nanoparticles (SLN) and oil-loaded SLN studied by spectrofluorometry and Raman spectroscopy. Pharm. Res. 22, 1887–1897 (2005)

    Article  Google Scholar 

  85. Y. Li, H.L. Wong, A.J. Shuhendler et al., Molecular interactions, internal structure and drug release kinetics of rationally developed polymer–lipid hybrid nanoparticles. J. Control. Release 128, 60–70 (2008)

    Article  Google Scholar 

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Authors and Affiliations

  1. Istanbul University, Faculty of Pharmacy, Department of Pharmaceutical Technology, Beyazit, 34116, Istanbul, Turkey

    Melike Üner

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  1. Department of Materials Engineering, Tarbiat Modares University, Tehran, Iran

    Mahmood Aliofkhazraei

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Üner, M. (2016). Characterization and Imaging of Solid Lipid Nanoparticles and Nanostructured Lipid Carriers. In: Aliofkhazraei, M. (eds) Handbook of Nanoparticles. Springer, Cham. https://doi.org/10.1007/978-3-319-15338-4_3

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