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Scanning Electrochemical Microscopy: A Multiplexing Tool for Electrochemical DNA Biosensing

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Handbook of Nanoelectrochemistry

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Abstract

Scanning electrochemical microscopy (SECM) translates the current generated by an electrochemical reaction occurring at a tip electrode scanned across a surface substrate into an image. SECM not only provides a simple electrochemical image of the conductive and/or insulating substrate but also provides kinetic information of the heterogeneous electron transfer reactions when the tip electrode approaches the surface. Applications including biosensing have been demonstrated. In this chapter, we will focus on recent advances in the application of SECM toward the label-free detection of base pair mismatches in DNA.

Despite having nanometer dimensions, the base pair mismatches along a DNA strand can be readily detected by SECM in an array format through exploiting the negative charge in the vicinity of self-assembled DNA films. The response can be amplified using metal ions to enhance the discrimination between matched and mismatched DNA films. This simple strategy has been used to probe the position of a single nucleotide mismatch, the type of the mismatch, and hybridization position of complementary strand and even allows the identification of various animal species.

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Author information

Authors and Affiliations

  1. Department of Physical and Environmental Sciences, University of Toronto Scarborough, 1265 Military Trail, Toronto, ON, Canada

    Mohtashim Hassan Shamsi & Heinz-Bernhard Kraatz

  2. Department of Chemistry, University of Toronto, 80St. George Street, Toronto, ON, Canada

    Mohtashim Hassan Shamsi & Heinz-Bernhard Kraatz

Authors
  1. Mohtashim Hassan Shamsi
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  2. Heinz-Bernhard Kraatz
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Corresponding author

Correspondence to Heinz-Bernhard Kraatz .

Editor information

Editors and Affiliations

  1. Department of Materials Engineering, Tarbiat Modares University, Tehran, Iran

    Mahmood Aliofkhazraei

  2. Manufacturing Engineering Department, College of Engineering and Computer Science, University of Texas Pan-American, Edinburg, Texas, USA

    Abdel Salam Hamdy Makhlouf

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Shamsi, M.H., Kraatz, HB. (2016). Scanning Electrochemical Microscopy: A Multiplexing Tool for Electrochemical DNA Biosensing. In: Aliofkhazraei, M., Makhlouf, A. (eds) Handbook of Nanoelectrochemistry. Springer, Cham. https://doi.org/10.1007/978-3-319-15266-0_35

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