Skip to main content

Orchids from Basilicata: The Scent

  • Living reference work entry
  • First Online:
Orchids Phytochemistry, Biology and Horticulture

Abstract

HS-SPME-GC-MS analysis of the scent of some orchid species found in Basilicata (Southern Italy) has been performed. The analysis of Platanthera species showed the presence of two different behaviors. The samples from Basilicata showed the presence of lilac derivatives, while samples from Calabria or Abruzzo had as main component benzyl benzoate. The analysis of Cephalanthera orchids showed that, probably, scent does not have a relevant role on the pollination strategy of the plants in comparison to the role of color. The analysis of Serapias orchids showed the presence of alkanes and alkenes but with a lower molecular weight than those reported in previous works on these species. Furthermore, α-amorphene was found as main component in Serapias cordigera and Serapias cordigera subsp. lucana. The analysis of Barlia robertiana showed a high variability in the composition of the scent.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Institutional subscriptions

References

  1. Pawliszyn J (1997) Solid-phase microextraction: theory and practice. Wiley, New York

    Google Scholar 

  2. Bentivenga G, D’Auria M, De Luca E, De Bona A, Mauriello G (2001) The use of SPME-GC-MS in the analysis of flavor of virgin olive oil. Riv Ital Sostanze Gr 78:157–162

    CAS  Google Scholar 

  3. Bentivenga G, D’Auria M, De Bona A, Mauriello G (2002) On the flavor of virgin olive oil. Riv Ital Sostanze Gr 79:101–105

    Google Scholar 

  4. Bentivenga G, D’Auria M, Mauriello G, Racioppi R, Viggiani L (2004) Characterization of virgin olive oils from Basilicata by using 1H-NMR and SPME-GC-MS. Riv Ital Sostanze Gr 81:86–89

    Google Scholar 

  5. D’Auria M, Emanuele L, Mauriello G, Racioppi R (2003) On the origin of “Goût de Lumiere” in champagne. J Photochem Photobiol A Chem 158:21–26

    Article  CAS  Google Scholar 

  6. Mauriello G, Capece A, D’Auria M, Garde-Cerdán T, Romano P (2009) SPME-GC method as a tool to differentiate VOC profiles in Saccharomyces cerevisiae wine yeasts. Food Microbiol 26:246–252

    Article  CAS  PubMed  Google Scholar 

  7. D’Auria M, Emanuele L, Racioppi R (2009) The effect of heat and light on the composition of some volatile compounds in wine. Food Chem 117:9–14

    Article  CAS  Google Scholar 

  8. Cellamare L, D’Auria M, Emanuele L, Racioppi R (2009) The effect of light on the composition of some volatile compounds in wine: an HS-SPME-GC-MS study. Int J Food Sci Technol 44:2377–2384

    Article  CAS  Google Scholar 

  9. Acquaviva V, D’Auria M, Racioppi R (2014) Changes in aliphatic ester composition in white wines during exposition to light. An HS-SPME-GC-MS study. J Wine Res 25:63–74

    Article  Google Scholar 

  10. D’Auria M, Mauriello G, Rana GL (2004) Volatile organic compounds from saffron. Flav Fragr J 19:17–23

    Article  CAS  Google Scholar 

  11. D’Auria M, Mauriello G, Racioppi R, Rana GL (2006) Use of SPME-GC-MS in the study of time evolution of the constituents of saffron aroma: modifications of the composition during storage. J Chromatogr Sci 44:18–21

    Article  Google Scholar 

  12. Mauriello G, Marino R, D’Auria M, Cerone G, Rana GL (2004) Determination of volatile organic compounds from truffles via SPME-GC-MS. J Chromatogr Sci 42:299–305

    Article  CAS  PubMed  Google Scholar 

  13. D’Auria M, Rana GL, Racioppi R, Laurita A (2012) Studies on volatile organic compounds of Tuber borchii and T. asa-foetida. J Chromatogr Sci 50:775–778

    PubMed  Google Scholar 

  14. D’Auria M, Racioppi R, Rana GL (2013) Volatile organic compounds of Schenella pityophilus. Nat Prod Res 27:41–44

    Article  PubMed  CAS  Google Scholar 

  15. D’Auria M, Racioppi R, Rana GL, Laurita A (2014) Studies on volatile organic compounds of some truffles and false truffles. Nat Prod Res 28:1709–1717

    Article  PubMed  CAS  Google Scholar 

  16. Mang SM, Racioppi R, Camele I, Rana GL, D’Auria M (2015) Use of volatile metabolite profiles to distinguish three Monilinia species. J Plant Pathol 97:55–59

    Google Scholar 

  17. Mang SM, Zotta T, Camele I, Racioppi R, D’Auria M, Rana GL (2017) Morphological, physico-chemical and molecular investigations on Tuber bellonae from Basilicata – Italy. J Anim Plant Sci 27:528–541

    CAS  Google Scholar 

  18. Bentivenga G, D’Auria M, Fedeli P, Mauriello G, Racioppi R (2004) SPME-GC-MS analysis of volatile organic compounds in honey from Basilicata. Evidence for the presence of pollutants from anthropogenic activities. Int J Food Sci Technol 39:1079–1086

    Article  CAS  Google Scholar 

  19. D’Auria M, Mauriello G, Racioppi R (2004) SPME-GC-MS analysis of horseradish (Armoracia rusticana). Ital J Food Sci 16:501–504

    Google Scholar 

  20. D’Auria M, Mauriello G, Marino R, Racioppi R (2005) Composition of volatile fraction from Thymus, Origanum, Lavandula and Acinos species. J Essent Oil Bear Plants 8:36–51

    Article  Google Scholar 

  21. D’Auria M, Racioppi R (2015) The effect of drying on the composition of volatile organic compounds in Rosmarinus officinalis, Laurus nobilis, Salvia officinalis and Thymus serpyllum. A HS-SPME-GC-MS study. J Essent Oil Bear Plants 18:1209–1223

    Article  CAS  Google Scholar 

  22. Celano G, D’Auria M, Xiloyannis C, Mauriello G, Baldassarre M (2006) Composition and seasonal variation of soluble cuticolar waxes in Actinidia deliciosa leaves. Nat Prod Res 20:701–709

    Article  CAS  PubMed  Google Scholar 

  23. D’Auria M, Racioppi R, Velluzzi V (2008) A comparison of results obtained using liquid injection and headspace solid-phase microextraction for crude oil analysis by GC with mass spectrometer detection. J Chromatogr Sci 46:332–338

    Article  PubMed  Google Scholar 

  24. D’Auria M, Racioppi R (2017) HS-SPME-GC-MS analysis of onion (Allium cepa L.) and shallot (Allium ascalonicum L.). Food Res 1:161–165

    Article  Google Scholar 

  25. D’Auria M, Emanuele L, Racioppi R, Stefanizzi N (2020) Determination of simple carbohydrates in beer using SPME, on fiber derivatization and gas chromatography mass spectrometry. Food Res 4:1156–1161

    Article  Google Scholar 

  26. Rosati L, Romano VA, Cerone L, Fascetti S, Potenza G, Bazzato E, Cillo D, Mecca M, Racioppi R, D’Auria M, Farris E (2020) Pollination features and floral volatiles of Gymnospermium scipetarum (Barberidaceae). J Plant Res 132:49–56

    Article  CAS  Google Scholar 

  27. Kaiser R (1993) Von Duft der Orchideen. Editiones Roche, Basel

    Google Scholar 

  28. Tollsten L, Bergström LG (1993) Fragrance chemotypes of Platanthera (Orchidaceae) – the result of adaptation to pollinating moths? Nord J Bot 13:607–613

    Article  Google Scholar 

  29. Peplys D, Ibarra F, Francke W, Löfstedt C (2002) Odour-mediated nectar foraging in the silver Y moth, Autographa gamma (Lepidoptera: Noctuidae): behavioural and electrophysiological responses to floral volatiles. Oikos 99:75–82

    Article  Google Scholar 

  30. Esposito F, Vereecken NJ, Gammella M, Rinaldi R, Laurent P, Tyteca D (2018) Characterization of sympatric Platanthera bifolia and Platanthera chlorantha (Orchidaceae) populations with intermediate plants. PeerJ 6:e4256

    Article  PubMed  PubMed Central  Google Scholar 

  31. D’Auria M, Lorenz R, Racioppi R, Romano VA (2017) Fragrance components of Platanthera bifolia subsp. osca. Nat Prod Res 31:1612–1619

    Article  PubMed  CAS  Google Scholar 

  32. D’Auria M, Lorenz R, Mecca M, Racioppi R, Romano VA, Viggiani L (2020) Fragrance components of Platanthera bifolia subsp. osca and Platanthera chlorantha collected in several sites in Italy. Nat Prod Res. https://doi.org/10.1080/14786419.2019.1593166

  33. Knudsen JT, Tollsten L, Bergström LG (1993) Floral scent – a checklist of volatile compounds isolated by head-space techniques. Phytochemistry 33:253–280

    Article  CAS  Google Scholar 

  34. Williams NH, Written WM (1983) Orchid floral fragrance and male euglossine bees: methods and advances in the fast sesquidecade. Biol Bull 163:355–395

    Article  Google Scholar 

  35. Claessens J, Kleynen J (2013) The pollination of European orchids: part 2: Cypripedium and Cephalanthera. J Hardy Orch Soc 10:114–120

    Google Scholar 

  36. D’Auria M, Lorenz R, Mecca M, Racioppi R, Romano VA (2020) Aroma components of Cephalanthera orchids. Nat Prod Res. https://doi.org/10.1080/14786419.2019.1616724

  37. Nilsson LA (1983) Mimesis of bellflower (Campanula) by the red helleborine orchid Cephalathera rubra. Nature 305:799–800

    Article  Google Scholar 

  38. Claessens J, Beentjes KK, Heijrman T, Miller J, Gravandeel B (2015) Beobachtungen von Miarus campanulae als Bestäuber von Cephalanthera rubra. J Eur Orch 47:77–87

    Google Scholar 

  39. Milet-Pinheiro P, Ayasse M, Dötterl S (2015) Visual and olfactory cues of Campanula (Campanulaceae) and their significance for host recognition by an oligolectic bee pollinator. PLoS One 10:e0128577

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  40. Lorenz R, Romano VA (2014) Beiträge über die morphologische Differenzierung in der Gattung Serapias: 1. Serapias cordigera in Süditalien. J Eur Orch 46:616–660

    Google Scholar 

  41. Pellegrino G, Luca A, Bellusci F, Musacchio A (2012) Comparative analysis of floral scents in four sympatric species of Serapias L. (Orchidaceae): clues on their pollination strategies. Plant Syst Evol 298:1837–1843

    Article  Google Scholar 

  42. Schiestl FP, Cozzolino S (2008) Evolution of sexual mimicry in the orchid subtribe orchidinae: the role of preadaptations in the attraction of male bees as pollinators. BMC Evol Biol 8:27

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  43. Vereecken NJ, Wilson CA, Hötling S, Schulz S, Banketov SA, Mardulyn P (2012) Pre-adaptations and the evolution of pollination by sexual deception: Cope’s rule of specialization revisited. Proc R Soc B 279:4786–4794

    Article  PubMed  PubMed Central  Google Scholar 

  44. D’Auria M, Lorenz R, Mecca M, Racioppi R, Romano VA (2020) The composition of the aroma of Serapias orchids in Basilicata (southern Italy). Nat Prod Res. https://doi.org/10.1080/14786419.2020.1713127

  45. Delforge P (2016) Orchidées d’Europe, d’Afrique du Nord et du Proche-Orient: la bible des orchidophiles, plus de 600 espèces et de nombreuses variétés et illustrées, 4th edn, revue et augmentée. Delachaux et Niestlé, Paris

    Google Scholar 

  46. Bartolucci F, Peruzzi L, Galasso G, Albano A, Alessandrini A, Ardenghi NMG, Astuti G, Bacchetta G, Ballelli S, Banfi E, Barberis G, Bernardo L, Bouvet D, Bovio M, Cecchi L, Di Pietro R, Domina G, Fascetti S, Fenu G, Festi F, Foggi B, Gallo L, Gottschlich G, Gubellini L, Iamonico D, Iberite M, Jiménez-Mejías P, Lattanzi E, Marchetti D, Martinetto E, Masin RR, Medagli P, Passalacqua NG, Peccenini S, Pennesi R, Pierini B, Poldini L, Prosser F, Raimondo FM, Roma-Marzio F, Rosati L, Santangelo A, Scoppola A, Scortegagna S, Selvaggi A, Selvi F, Soldano A, Stinca A, Wagensommer RP, Wilhalm T, Conti F (2018) An updated checklist of the vascular flora native to Italy. Plant Biosyst 152:179–303

    Article  Google Scholar 

  47. Vereecken NJ, Dafni A, Cozzolino S (2010) Pollination syndromes in Mediterranean orchids – implications for speciation, taxonomy and conservation. Bot Rev 76:220–240

    Article  Google Scholar 

  48. Doneddu M (2015) Osservazioni sull’impollinazione di Barlia robertiana ad opera dei coleotteri Tropinota squalida e Oxythyrea funesta (Cetoniidae) in Sardegna. GIROS Orch Spont Eur 58:262–265

    Google Scholar 

  49. Smithson A, Gigord LDB (2001) Are there fitness advantages in being a rewardless orchid? Reward supplementation experiments with Barlia robertiana. Proc R Soc Lond B Biol Sci 268:1435–1441

    Article  CAS  Google Scholar 

  50. McBrien HL, Millar JG, Rice RE, McElfresh JS, Cullen E, Zalom FG (2002) Sex attractant pheromone of the red-shouldered stink bug Thyanta pallidovirens: a pheromone blend with multiple redundant components. J Chem Ecol 28:1797–1818

    Article  CAS  PubMed  Google Scholar 

  51. Tulp M, Bohlin L (2005) Rediscovery of known natural compounds: nuisance or goldmine? Bioorg Med Chem 13:5274–5282

    Article  CAS  PubMed  Google Scholar 

  52. Gallego E, Gelabert A, Roca FJ, Perales JF, Guardino X (2012) Identification of volatile organic compounds (VOC) emitted from three European orchid species with different pollination strategies: two deceptive orchids (Himantoglossum robertianum and Ophrys apifera) and a rewarding (Gymnadenia conopsea). J Biodivers Environ Sci 2:18–29

    Google Scholar 

  53. Salzmann CC, Cozzolino S, Schiestl FP (2007) Floral scent in food-deceptive orchids: species specificity and sources of variability. Plant Biol 9:720–729

    Article  CAS  PubMed  Google Scholar 

  54. Jersáková J, Johnson SD, Kindlmann P (2006) Mechanisms and evolution of deceptive pollination in orchids. Biol Rev 81:219–235

    Article  PubMed  Google Scholar 

  55. Moya S, Ackerman JD (1993) Variation in the floral fragrance of Epidendrum ciliare (Orchidaceae). Nord J Bot 13:41–47

    Article  CAS  Google Scholar 

  56. Smithson A, Macnair MR (1997) Negative frequency-dependent selection by pollinators on artificial flowers without rewards. Evolution 51:715–723

    Article  PubMed  Google Scholar 

  57. Ackerman JD (1986) Mechanisms and evolution of food-deceptive pollination systems in orchids. Lindleyana 1:108–113

    Google Scholar 

  58. Johnson SD, Nilsson LA (1999) Pollen carryover, geitonogamy, and the evolution of deceptive pollination systems in orchids. Ecology 80:2607–2619

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Maurizio D’Auria .

Editor information

Editors and Affiliations

Section Editor information

Rights and permissions

Reprints and permissions

Copyright information

© 2020 Springer Nature Switzerland AG

About this entry

Check for updates. Verify currency and authenticity via CrossMark

Cite this entry

D’Auria, M., Fascetti, S., Racioppi, R., Romano, V.A., Rosati, L. (2020). Orchids from Basilicata: The Scent. In: Merillon, JM., Kodja, H. (eds) Orchids Phytochemistry, Biology and Horticulture. Reference Series in Phytochemistry. Springer, Cham. https://doi.org/10.1007/978-3-030-11257-8_32-1

Download citation

  • DOI: https://doi.org/10.1007/978-3-030-11257-8_32-1

  • Received:

  • Accepted:

  • Published:

  • Publisher Name: Springer, Cham

  • Print ISBN: 978-3-030-11257-8

  • Online ISBN: 978-3-030-11257-8

  • eBook Packages: Springer Reference Chemistry and Mat. ScienceReference Module Physical and Materials ScienceReference Module Chemistry, Materials and Physics

Publish with us

Policies and ethics