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In Vitro Systems of Selected Eryngium Species (E. planum, E. campestre, E. maritimum, and E. alpinum) for Studying Production of Desired Secondary Metabolites (Phenolic Acids, Flavonoids, Triterpenoid Saponins, and Essential Oil)

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Plant Cell and Tissue Differentiation and Secondary Metabolites

Part of the book series: Reference Series in Phytochemistry ((RSP))

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Abstract

There are four Eryngium species native to Poland, namely, E. planum and E. campestre – rare taxa, E. maritimum and E. alpinum – endangered and protected taxa belonging to the subfamily Saniculoideae of Apiaceae family. The phytochemical investigations revealed the presence of various groups of bioactive compounds, that is, triterpenoid saponins, phenolic acids, flavonoids, coumarin derivatives, the essential oil, polyacetylenes, phytosterols, and ecdysteroids. Plant in vitro cultures of those rare and endangered species as well as biotechnological methods of application may provide biomass with the enhanced accumulation of desired secondary metabolites without collecting plants from natural sites. Protocols of micropropagation with genome size stability confirmation and different types of cultures – organ, callus, and cell suspension cultures – were developed with the intent to achieve constant, uniform, and renewable biomass with the higher accumulation of polyphenols and triterpenoid saponins. Both soil-grown plants and in vitro systems were analyzed for the presence and content of main secondary metabolites. It was shown that in vitro-derived plantlets also showed biological activities. The phytochemical and biological studies of Eryngium species show their potential as valuable medicinal plants.

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Abbreviations

2,4-D:

2,4-Dichlorophenoxyacetic acid

ABTS:

2,2′-Azinobis-3-ethylbenzotiazo-line-6-sulfonic acid

AChE:

Acetylcholinesterase

BAP:

Benzylaminpurine

BuChE:

Butyrylcholinesterase

CA:

Caffeic acid

CGA:

Chlorogenic acid

DPPH:

2,2-Diphenyl-1-picrylhydrazyl

DW:

Dry weight

EC50:

Half maximal effective concentration

ES1:

3-O-β-d-Glucopyranosyl-(1→2)-β-d-glucuronopyranosyl-21-O-acetyl,22-O-angeloyl-R1-barrigenol

ES2:

3-O-β-d-Glucopyranosyl-(1→2)-β-d-glucuronopyranosyl-22-O-angeloyl-R1-barrigenol

ES3:

3-O-β-d-Glucopyranosyl-(1→2)-β-d-glucuronopyranosyl-22-O-angeloyl-A1-barrigenol

GA3:

Gibberellic acid

GC:

Gas chromatography

GC-FID-MS:

Gas chromatography-flame ionization-mass spectrometer

GC-MS:

Gas chromatography-mass spectrometer

HPLC:

High performance liquid chromatography

HPLC-DAD:

High performance liquid chromatography – diode-array detector

IAA:

Indole-3-acetic acid

IBA:

Indole-3-butyric acid

IC50:

Half maximal inhibitory concentration

KIN:

Kinetin

LC-MS:

Liquid chromatography-mass spectrometer

MeJa:

Methyl jasmonate

MIC:

Minimal inhibitory concentration

MS:

Murashige and Skoog medium

NAA:

Naphthaleneacetic acid

NMR:

Nuclear magnetic resonance

RA:

Rosmarinic acid

TAC:

Total antioxidant capacity

TBA:

Thiobarbituric acid

UHPLC:

Ultra-high performance liquid chromatography

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Authors and Affiliations

  1. Department of Pharmaceutical Botany and Plant Biotechnology, Poznan University of Medicinal Sciences, Poznań, Poland

    Małgorzata Kikowska & Barbara Thiem

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  1. Małgorzata Kikowska
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  2. Barbara Thiem
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Correspondence to Barbara Thiem .

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Editors and Affiliations

  1. Department of Botany, M.L.Sukhadia university, Udaipur, Rajasthan, India

    Kishan Gopal Ramawat

  2. Department of Pharmaceutical Botany, Jagiellonian University, Medical College, Kraków, Poland

    Halina Maria Ekiert

  3. Amarillo, TX, USA

    Shaily Goyal

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Kikowska, M., Thiem, B. (2020). In Vitro Systems of Selected Eryngium Species (E. planum, E. campestre, E. maritimum, and E. alpinum) for Studying Production of Desired Secondary Metabolites (Phenolic Acids, Flavonoids, Triterpenoid Saponins, and Essential Oil). In: Ramawat, K., Ekiert, H., Goyal, S. (eds) Plant Cell and Tissue Differentiation and Secondary Metabolites. Reference Series in Phytochemistry. Springer, Cham. https://doi.org/10.1007/978-3-030-11253-0_29-2

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  • DOI: https://doi.org/10.1007/978-3-030-11253-0_29-2

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  • Publisher Name: Springer, Cham

  • Print ISBN: 978-3-030-11253-0

  • Online ISBN: 978-3-030-11253-0

  • eBook Packages: Springer Reference Chemistry and Mat. ScienceReference Module Physical and Materials ScienceReference Module Chemistry, Materials and Physics

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    ) for Studying Production of Desired Secondary Metabolites (Phenolic Acids, Flavonoids, Triterpenoid Saponins, and Essential Oil)
    Published:
    17 January 2020

    DOI: https://doi.org/10.1007/978-3-030-11253-0_29-2

  2. Original

    ) for Studying Production of Desired Secondary Metabolites (Phenolic Acids, Flavonoids, Triterpenoid Saponins, and Essential Oil)
    Published:
    11 December 2019

    DOI: https://doi.org/10.1007/978-3-030-11253-0_29-1

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