Glossary
Hydrogen; microalgae; Chlamydomonas reinhardtii; D1 protein; PSII; Fluorescence yield; Hydrogenase; Anaerobiosis
Definition of the Subject
In recent years, the increasing depletion of fossil resources has increased the necessity to search for alternative sources of energy. What seems imperative is that all efforts have to be addressed to improve the utilization of renewable source of energy. It is well known that under particular conditions, microalgae are capable to produce hydrogen as almost pure biogas. In this respect, the goal would be to use the sole solar light energy and water, a clean and green sustainable source of energy. However, this process has some limitation. The main bottleneck is represented by the reduced photosynthetic efficiency of photosynthetic cells to convert solar light energy into chemical energy (or hydrogen). Many studies have been carried out all over the years in order to solve this problem, but at the moment, only a small improvement has been...
Bibliography
Primary Literature
Gaffron H, Rubin J (1942) Fermentative and photochemical production of hydrogen in algae. J Gen Physiol 26:219–240
Zhu XG, Long SP, Ort DR (2008) What is the maximum efficiency with which photosynthesis can convert energy into biomass? Curr Opin Biotechnol 19:153–159
Faraloni C, Torzillo G (2013) Xanthophyll cycle induction by anaerobiosis conditions under low light in Chlamydomonas reinhardtii. J Appl Phycol 25:1457–1471
Melis A (2009) Solar energy conversion efficiencies in photosynthesis: minimizing the chlorophyll antennae to maximize efficiency. Plant Sci 177:272–280
Pirt SJ, Lee KYK, Richmond A, Pirt Watts M (1980) The photosynthesis efficiency of Chlorella biomass grown with reference to solar energy utilisation. J Chem Technol Biotechnol 30:25–34
Srirangan K, Pyne ME, Perry Chou C (2011) Biochemical and genetic engineering strategies to enhance hydrogen production in photosynthetic algae and cyanobacteria. Bioresour Technol 102(18):8589–8560
Bhattacharya D, Yoon HS, Hacket JD (2004) Photosynthetic eukaryotes inite: endosymbiosis connects the dots. Bioessays 26:50–60
Guiry MD (2012) How many species of algae are there? J Phycol 48:1057–1063
Norton TA, Melkonian M, Andersen RA (1996) Algal biodiversity. Phycologia 35:308–326
Borowitzka MA (2013) High value product from microalgae- their development and commercialization. J Appl Phycol 25:743–756
Leu S, Boussiba S (2014) Advances in the production of high value products by microalgae. Int Biotechnol 10:169–183
Davis R, Aden A, Pienkova PT (2011) Techno-economic analysis of autotrophic microalgae for fuel production. Appl Energy 88:3524–3531
Finazzi G, Furia A, Barbagallo RM, Forti G (1999) State transitions, cyclic and linear transport and photophosphorylation in Chlamydomonas reinhardtii. Biochim Biophys Acta 1413:117–129
Meyer J (2007) FeFe hydrogenases and their evolution: a genomic perspective. Cell Mol Life Sci 64(9):1063–1084
Debajyoti D, Debojyoti D, Surabhi C, Sanjoy KB (2005) Hydrogen production by Cyanobacteria. Microb Cell Factories 4:36
Kim DH, Kim MS (2011) Hydrogenases for biological hydrogen production. Bioresour Technol 102(18):8423–8431
Florin L, Tsokoglou A, Happe T (2001) A novel type of iron hydrogenase in the green alga Scenedesmus obliquus is linked to the photosynthetic electron transport chain. J Biol Chem 276(9):6125–6132
Vignais PM, Billoud B, Meyer J (2001) Classification and phylogeny of hydrogenases. FEMS Microbiol Rev 25(4):455–501
Nicolet Y, de Lacey AL, Vernède X, Fernandez VM, Hatchikian EC, Fontecilla-Camps JC (2001) Crystallographic and FTIR spectroscopic evidence of changes in Fe coordination upon reduction of the active site of the Fe-only hydrogenase from Desulfovibrio desulfuricans. J Am Chem Soc 123:1596–1601
Happe T, Kaminski A (2002) Differential regulation of the Fe-hydrogenase during anaerobic adaptation in the green alga Chlamydomonas reinhardtii. Eur J Biochem 269:1022–1032
Forestier M, King P, Zhang L, Posewitz M, Schwarzer S, Happe T, Ghirardi ML, Seibert M (2003) Expression of two [Fe]-hydrogenases in Chlamydomonas reinhardtii under anaerobic conditions. Eur J Biochem 270:2750–2758
Ogata H, Lubitz W, Higuchi Y (2009) [NiFe] hydrogenases:structural and spectroscopic studies of the reaction mechanism. Dalton Trans 37:7577–7587
Meuser JE, D'Adamo S, Jinkerson RE, Mus F, Yang W, Ghirardi ML, Seibert M, Grossman AR, Posewitz MC (2012) Genetic disruption of both Chlamydomonas reinhardtii [FeFe]-hydrogenases: Insight into the role of HYDA2 in H2 production. Biochem Biophys Res Commun 417(2):704–709
Boyer ME, Stapleton JA, Kuchenreuther JM, Wang CW, Swartz JR (2008) Cell-free synthesis and maturation of [FeFe] hydrogenases. Biotechnol Bioeng 99(1):59–67
Posewitz MC, King PW, Smolinski SL, Smith RD, Ginley AR, Ghirardi ML (2005) Identification of genes required for hydrogenase activity in Chlamydomonas reinhardtii. Biochem Soc Trans 33:102–104
Böck A, King PW, Blokesch M, Posewitz MC (2006) Maturation of hydrogenases. Adv Microb Physiol 51:1–72
Skjånes K, Pinto F, Lindblad P (2010) Evidence for transcription of three genes with characteristics of hydrogenases in the green alga Chlamydomonas noctigama. Int J Hydrog Energy 35(3):1074–1088
Posewitz MC, Mulder DW, Peters JW (2008) New frontiers in hydrogenase structure and biosynthesis. Curr Chem Biol 2:178–199
English CM, Eckert C, Brown K, Seibert M, King PW (2009) Recombinant and in vitro expression system for hydrogenases: new frontiers in basic and applied studies for biological and synthetic production. Dalton Trans 45:9970–9978
Bothe H, Schmitz O, Yates MG, Newton WE (2010) Nitrogen fixation and hydrogen metabolism in cyanobacteria. Microbiol Mol Biol Rev 74(4):529–551
Carrieri D, Momot D, Brasg IA, Ananyev G, Lenz O, Bryant DA, Dismukes GC (2010) Boosting autofermentation rates and product yields with sodium stress cycling. Application to renewable fuel production by cyanobacteria. Appl Environ Microbiol 76:6455–6462
Skizim NJ, Ananyev GM, Krishnan A, Dismukes GC (2012) Metabolic Pathways for Photobiological Hydrogen Production by Nitrogenase- and Hydrogenase-containing Unicellular Cyanobacteria Cyanothece. J Biol Chem 287(4):2777–2786
Shestakov SV, Mikheeva LE (2006) Genetic control of hydrogen metabolism in cyanobacteria. Russ J Genet 42(11):1272–1284
Oncel S (2013) Microalgae for a macroenergy world. Renew Sust Energ Rev 26:241–264
Antal TK, Krendeleva TE, Laurinavichene TV, Makarova VV, Ghirardi ML, Rubin AB, Tsygangov AA, Seibert M (2003) The dependence of algal H2 production on photosystem II and O2 consumption in sulfur-deprived Chlamydomonas reinhardtii cells. BBA 1607:153–160
Melis A, Zhang L, Forestier M, Ghirardi M, Seibert M (2000) Sustained photobiological hydrogen gas production upon reversible inactivation of oxygen evolution in the green alga Chlamydomonas reinhardtii. Plant Physiol 122:127–135
Scoma A, Giannelli L, Faraloni C, Torzillo G (2012) Outdoor H2 production in a 50-liter tubular photobioreactor by means of a sulfur-deprived culture of the microalga Chlamydomonas reinhardtii. JBiotechnol 157:620–627
Oncel S, Kose A, Faraloni C, Imamoglu E, Elibol M, Torzillo G, Vardar Sukan F (2015) Biohydrogen production from model microalgae Chlamydomonas reinhardtii: a simulation of environmental conditions for outdoor experiments. Int J Hydrog Energy 40(24):7502–7510. Special Issue
Philipps G, Happe T, Hemschemeier A (2012) Nitrogen deprivation results in photosynthetic hydrogen production in Chlamydomonas reinhardtii. Planta 235:729–745
Hemschemeier A, Jacobs J, Happe T (2008) The pyruvate formate-lyase (Pfl1) of Chlamydomonas reinhardtii—a biochemical and physiological characterization of a typically bacterial enzyme in a eukaryotic alga. Eukaryot Cell 7:518–526
Mus F, Cournac L, Cardettini V, Caruana A, Peltier G (2005) Inhibitor studies on non-photochemical PQ reduction and H2 photoproduction in Chlamydomonas reinhardtii. Biochim Biophys Acta 1708:322–332
Terashima M, Specht M, Naumann B, Hippler M (2010) Characterizing the anaerobic response of Chlamydomonas reinhardtii by quantitative proteomics. Mol Cell Proteomics 9(7):1514–1532
Terashima M, Specht M, Hippler M (2011) The chloroplast proteome: a survey from the Chlamydomonas reinhardtii perspective with a focus on distinctive features. Curr Genet 57(3):151–168
Ghirardi ML, Dubini A, Yu J, Maness PC (2009) Photobiological hydrogen-producing systems. Chem Soc Rev 38:52–61
Hallenbeck P, Abo-Hashesh M, Ghosh D (2012) Strategies for improving biological hydrogen production. Bioresour Technol 110:1–9
Melis A (2007) Photosynthetic H2 metabolism in Chlamydomonas reinhardtii (unicellular green algae). Planta 226:1075–1086
Mathews J, Wang G (2009) Metabolic pathway engineering for enhanced biohydrogen production. Int J Hydrogen Energ 34:7404–7416
Carrieri D, Wawrousek K, Eckert C, Yu J, Maness PC (2011) The role of the bidirectional hydrogenase in cyanobacteria. Bioresour Technol 102(18):8368–8377
Wykoff DD, Davies JP, Melis A, Grossman AR (1998) The Regulation of Photosynthetic Electron Transport during Nutrient Deprivation in Chlamydomonas reinhardtii. Plant Physiol 117:129–139
Fouchard S, Hemschemeier A, Caruana A, Pruvost J, Legrand J, Happe T, Peltier G, Cournac L (2005) Autotrophic and mixotrophic hydrogen photoproduction in sulfur-deprived Chlamydomonas cells. Appl Environ Microbiol 71(10):6199–6205
Faraloni C, Torzillo G (2010) Phenotypic characterization and hydrogen production in Chlamydomonas reinhardtii QB-binding D1-protein mutants under sulfur starvation: changes in chl fluorescence and pigment composition. J Phycol 46:788–799
Chochois V, Dauvillée D, Beyly A, Tolleter D, Cuiné S, Timpano H, Ball S, Cournac L, Peltier G (2009) Hydrogen Production in Chlamydomonas: Photosystem II-dependent and-independent pathways differ in their requirement for starch metabolism. Plant Physiol 151:631–640
Ghirardi ML, Zhang L, Lee JW, Flynn T, Seibert M, Greenbaum E, Melis A (2000) Microalgae: a green source of renewable H2. TIBTECH 18:506–511
Kruse O, Rupprecht J, Bade KP, Thomas-Hall S, Schenk PM, Finazzi G, Hankamer B (2005) Improved photobiological H2 production in engineered green algal cells. J Biol Chem 280:34170–34176
Scoma A, Krawietz D, Faraloni C, Giannelli L, Happe T, Torzillo G (2012) Sustained H2 production in a Chlamydomonas reinhardtii D1 protein mutant. J Biotechnol 157:613–619
Volgusheva A, Styring S, Mamedov F (2013) Increased photosystem II stability promotes H2 production in sulfur-deprived Chlamydomonas reinhardtii. PNAS 110(18):7223–7228
Melis A, Seibert M, Happe T (2004) Genomics of green algal hydrogen research. Photosynth Res 82:277–288
Torzillo G, Scoma A, Faraloni C, Ena A, Johanningmeier U (2009) Increased hydrogen photoproduction by means of a sulfur-deprived Chlamydomonas reinhardtii D1 protein mutant. Int J Hydrog Energy 34:4529–4536
Trebst A (1987) The three-dimensional structure of the herbicide binding niche on the reaction center polypeptides of photosystem II. Z Naturforsch 42:742
Sobolev V, Edelman M (1995) Modeling the Quinone-B binding site of the photosystem-II-reaction center using notions of complementary and contact-surface between atoms. Proteins Truct Funct Genet 21:214–225
Kettunen R, Tyystjärvi E, Aro EM (1996) Degradation pattern of photosystem II reaction center protein D1 in intact leaves. Plant Physiol 111:1183–1190
Bayro-Kaiser V, Nelson N (2016) Temperature-sensitive PSII: a novel approach for sustained photosynthetic hydrogen production. Phptos Res. https://doi.org/10.1007/s1120-016-0232-3
Cournac L, Latouche G, Cerovic Z, Redding K, Ravenel J, Peltier G (2002) In vivo interactions between photosynthesis, mitorespiration, and chlororespiration in Chlamydomonas reinhardtii. Plant Physiol 129:1921–1928
Cardol P, Gloire G, Havaux M, Remacle C, Matagne R, Franck F (2003) Photosynthesis and state transitions in mitochondrial mutants of Chlamydomonas reinhardtii affected in respiration. Plant Physiol 133:2010–2020
Endo T, Asada K (1996) Dark induction of the nonphotochemical quenching of chlorophyll fluorescence by acetate in Chlamydomonas reinhardtii. Plant Cell Physiol 37(4):551–555
Doebbe A, Rupprecht J, Beckmann J, Mussgnug JH, Hallmann A, Hankmer B, Kruse O (2007) Functional integration of the HUP1 hexose symporter gene into the genome of C. reinhardtii: impacts on biological H2 production. J Biotechnol 131:27–33
Wu S, Xu L, Wang R, Liu X, Wang QW (2011) A high yield mutant of Chlamydomonas reinhardtii for photoproduction of hydrogen. Int J Hydrog Energy 36:14134–14140
Chien LF, Kuo TT, Liu BH, Lin HD, Feng TY, Huang CC (2012) Solar-to-bioH2 production enhanced by homologous overexpression of hydrogenase in green alga Chlorella sp. DT. Int J Hydrog Energy 2012:17738–17748
Kosourov SN, Ghirardi ML, Seibert M (2011) A truncated antenna mutant of Chlamydomonas reinhardtii can produce more hydrogen than the parental strain. Int J Hydrog Energy 36:2044–2048
Strasser R, Srivastava A, Govindjee (1995) Polyphasic chlorophyll a fluorescence transient in plants and cyanobacteria. Photochem Photo-Dermatology 61:32–42
Finazzi G, Barbagallo RP, Bergo E, Barbato R, Forti G (2001) Photoinhibition of Chlamydomonas reinhardtii in state 1 and state 2. J Biol Chem 276:22251–22257
Bennoun P (2002) The present model for chlororespiration. Photosynth Res 73:273–277
Toth SZ, Schansker G, Garab G, Strasser RJ (2007) Photosynthetic electron transport activity in heat treated barley leaves, the role of internal alternative electron donors to photosystem II. BBA-Bioenergetics 1767:295–305
Antal TK, Volgusheva AA, Kukarskikh GP, Krendeleva TE, Tusov VB, Rubin AB (2006) Examination of chlorophyll fluorescence in sulfur-deprived cells of Chlamydomonas reinhardtii. Biofizika 51(2):292–298
Antal TK, Krendeleva TE, Rubin AB (2007) Study of photosystem 2 heterogeneity in the sulfur-deficient green alga Chlamydomonas reinhardtii. Photosynth Res 94(1):13–22
Oh YK, Raj SM, Jung GY, Park S (2011) Current status of the metabolic engineering of microorganisms for biohydrogen production. Bioresour Technol 102:8357–8367
Johanningmeier U, Heiss S (1993) Construction of a Chlamydomonas reinhardtii mutant with an intronless psbA gene. Plant Mol Biol 22(1):91–99
Books and Reviews
Edelman M, Mattoo AK, Marder JB (1984) Three hats of the rapidly metabolized 32 kD protein thylakoids. In: Ellis RT (ed) Chloroplast biogenesis. Cambridge University Press, Cambridge, pp 283–302
Ohad I, Kren N, Zer H, Gong H, Mor TS, Gal A, Tal S, Domovich Y (1994) Light-induced degradation of the photosystem II reaction centre D1 protein in vivo, an integrative approach. In: Backer NR, Bowyer JR (eds) Photoinhibition of photosynthesis, from molecular mechanisms to the field. Bios Scientific Publishers, Oxford, pp 161–178
Torzillo G, Seibert M (2013) Hydrogen production by microalgae. In: Richmond A, Hu Q (eds) Handbook of microalgal culture: applied phycology and biotechnology, 2nd edn. Wiley, Oxford, pp 417–444
Torzillo G, Scoma A, Faraloni C, Giannelli L (2014) Advances in the biotechnology of hydrogen production with the microalga Chlamydomonas reinhardtii. Crit Rev Biotechnol 35(4):485–496
Vermaas WFJ, Ikeuchi M (1991) Photosystem II. In: Bogorad L, Vasil IK (eds) The photosynthetic apparatus, molecular biology and operation, Cell culture and somatic cell genetics of plants, vol 7B. Academic Press, San Diego, pp 25–111
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Section Editor information
Rights and permissions
Copyright information
© 2018 Springer Science+Business Media LLC
About this entry
Cite this entry
Faraloni, C., Torzillo, G. (2018). Genetic Optimization for Increasing Hydrogen Production in Microalgae. In: Meyers, R. (eds) Encyclopedia of Sustainability Science and Technology. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-2493-6_950-1
Download citation
DOI: https://doi.org/10.1007/978-1-4939-2493-6_950-1
Received:
Accepted:
Published:
Publisher Name: Springer, New York, NY
Print ISBN: 978-1-4939-2493-6
Online ISBN: 978-1-4939-2493-6
eBook Packages: Springer Reference Earth and Environm. ScienceReference Module Physical and Materials ScienceReference Module Earth and Environmental Sciences