Malaria Diagnostic Platform, ELISA
Enzyme-linked immunosorbent assay is a powerful diagnostic tool which, however, has only limited application in malaria diagnostics and research but may be the answer to a number of upcoming questions. This entry discusses potential applications and limitations of antigen and antibody detection by ELISA as a malaria diagnostic platform.
In the history of the analysis of biomolecules, immunoassays together with various chromatographic and more recently molecular techniques have probably been the most commonly used technologies. Immunoassays are well suited for qualitative and quantitative assays of a single, well-defined molecular group. As compared to most other available methods, immunoassays can be developed that use less complex equipment and can be used in a wider range of testing environments.
Immunoassays take advantage of a natural phenomenon in order to quantify...
KeywordsRapid Diagnostic Test Mass Drug Administration Entomological Inoculation Rate Malaria Diagnosis Indirect Fluorescent Antibody Test
- Basco L. Field application of in vitro assays sensitivity of human malaria parasites antimalarial drugs. Geneva: World Health Organization; 2007.Google Scholar
- Drakeley CJ, Corran PH, Coleman PG, Tongren JE, McDonald SL, Carneiro I, Malima R, Lusingu J, Manjurano A, Nkya WM, Lemnge MM, Cox J, Reyburn H, Riley EM. Estimating medium- and long-term trends in malaria transmission by using serological markers of malaria exposure. Proc Natl Acad Sci U S A. 2008;102(14):5108–13.CrossRefGoogle Scholar
- Stewart L, Gosling R, Griffin J, Gesase S, Campo J, Hashim R, Masika P, Mosha J, Bousema T, Shekalaghe S, Cook J, Corran P, Ghani A, Riley EM, Drakeley C. Rapid assessment of malaria transmission using age-specific sero-conversion rates. PLoS One. 2009;4(6):e6083.PubMedCentralCrossRefPubMedGoogle Scholar
- World Health Organization. Screening donated blood for transfusion-transmissible infections: recommendations. Geneva: World Health Organization; 2010.Google Scholar