Molecular Life Sciences

Living Edition
| Editors: Robert D. Wells, Judith S. Bond, Judith Klinman, Bettie Sue Siler Masters, Ellis Bell

Target-Primed Mobilization Mechanisms

Living reference work entry
DOI: https://doi.org/10.1007/978-1-4614-6436-5_156-1

Synonyms

Synopsis

Some genetic elements utilize a free 3′ OH at a new genetic location to prime DNA synthesis, resulting in a copy of the element in the target DNA. In a simple example of this mechanism, homing endonucleases are encoded within mobile elements, which are used to create a double-strand DNA break(s) in a target DNA molecule. By creating a double-strand DNA break(s), the host genome is activated for homologous recombination, and the mobile element is copied into that DNA site; the 3′ ends from one of the broken DNA are used to prime synthesis of the element. Other mobile elements use a process termed target-primed reverse transcription to move via an RNA intermediate. Following transcription of the DNA element, the RNA copy is reverse transcribed into a new DNA site, using a nick in the DNA at that site to prime reverse transcription. There are two major classes of elements that move by a target-primed reverse transcription mechanism,...

Keywords

Zinc Recombination Adenosine Sine Transesterification 
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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  1. 1.Molecular Control and Genetics Section, Gene Regulation and Chromosome Biology LaboratoryNational Cancer Institute, National Institutes of HealthFrederickUSA
  2. 2.Department of MicrobiologyCornell UniversityIthacaUSA