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Encyclopedia of Metagenomics pp 1–11Cite as

DNA Methylation Analysis by Pyrosequencing

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Synonyms

Quantitative sequencing by synthesis

Definition

Pyrosequencing is a sequencing-by-synthesis method that quantitatively monitors the real-time incorporation of nucleotides using an enzymatic conversion of pyrophosphate into a proportional light signal. Quantitative measures are crucial for applications such as the analysis of DNA methylation patterns, which are intensively studied in various developmental and pathological contexts as well as for bacterial identification and determination of allelic imbalance.

Introduction

While Sanger sequencing has been the “gold standard” for the identification of sequence variants for a long time, pyrosequencing with its improved ability for quantification, decreased limit of detection and accelerated workflow leading to a shorter time to results, has become a valuable alternative notably for many clinical and diagnostic applications. Pyrosequencing is a sequencing-by-synthesis method, where nucleotides are incorporated complementary to a...

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Authors and Affiliations

  1. Laboratory for Epigenetics and Environment, Centre National de Génotypage, CEA- Institut de Génomique, 91000, Evry, France

    Florence Busato & Jörg Tost

  2. Laboratory for Epigenetics and Environnement, Centre National de Génotypage, CEA-Institut de Génomique, Bâtiment G2, 2 rue Gaston Crémieux, CP 5721, 91057, Evry Cedex, France

    Jörg Tost

Authors
  1. Florence Busato
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  2. Jörg Tost
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Corresponding author

Correspondence to Jörg Tost .

Editor information

Editors and Affiliations

  1. J. Craig Venter Institute (JCVI), Rockville, Maryland, USA

    Karen E. Nelson

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© 2014 Springer Science+Business Media New York

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Busato, F., Tost, J. (2014). DNA Methylation Analysis by Pyrosequencing. In: Nelson, K. (eds) Encyclopedia of Metagenomics. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-6418-1_799-1

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  • DOI: https://doi.org/10.1007/978-1-4614-6418-1_799-1

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  • Publisher Name: Springer, New York, NY

  • Online ISBN: 978-1-4614-6418-1

  • eBook Packages: Springer Reference Biomedicine and Life SciencesReference Module Biomedical and Life Sciences

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