DNA Methylation Analysis by Pyrosequencing
Pyrosequencing is a sequencing-by-synthesis method that quantitatively monitors the real-time incorporation of nucleotides using an enzymatic conversion of pyrophosphate into a proportional light signal. Quantitative measures are crucial for applications such as the analysis of DNA methylation patterns, which are intensively studied in various developmental and pathological contexts as well as for bacterial identification and determination of allelic imbalance.
KeywordsAngelman Syndrome Human Microbiome Project Imprint Control Region Pyrosequencing Assay Conventional Sanger Sequencing
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