DNA Methylation Analysis by Pyrosequencing
Pyrosequencing is a sequencing-by-synthesis method that quantitatively monitors the real-time incorporation of nucleotides using an enzymatic conversion of pyrophosphate into a proportional light signal. Quantitative measures are crucial for applications such as the analysis of DNA methylation patterns, which are intensively studied in various developmental and pathological contexts as well as for bacterial identification and determination of allelic imbalance.
KeywordsAdenosine Adduct Sodium Hydroxide Lactose Adenine
- Kristensen LS, Treppendahl MB, Asmar F, Girkov MS, Nielsen HM, Kjeldsen TE, et al. Investigation of MGMT and DAPK1 methylation patterns in diffuse large B-cell lymphoma using allelic MSP-pyrosequencing. Sci Rep. 2013;3.Google Scholar
- Marsh S, editor. Pyrosequencing protocols, methods in molecular biology vol 373. Totowa: Humana Press; 2007.Google Scholar