Taxonomic Classification of Metagenomic Shotgun Sequences with CARMA3
CARMA3 is a program to assign taxonomic identifiers to metagenomic sequences of unknown taxonomic origin.
The vast majority of microbes cannot be cultivated in a monoculture and thus cannot be sequenced by means of traditional methods. To explore these microbes, they have to be analyzed within their natural microbial communities. High-throughput sequencing (HTS) technologies like Roche’s 454 sequencing, ABI’s SOLiD, or Illumina’s Genome Analyzer make it possible to sequence microbial DNA samples of such communities, called metagenomes. Due to the restricted read lengths produced by these technologies, reconstruction of complete genomic sequences from a metagenome is impossible. However, by comparing the metagenomic fragments with sequences of known function, it is possible to analyze the biological diversity and the underlying metabolic pathways in microbial communities.
To infer the taxonomic origin of metagenomic...
KeywordsTaxonomic Classification Taxonomic Rank Metagenomic Sequence Lower Common Ancestor Lower Common Ancestor
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