Open Resource Metagenomics
Open resource metagenomics encompasses the emerging resource sharing system that facilitates distribution of metagenomic libraries throughout the research community. These libraries are constructed from DNA isolated directly from environmental samples. Broad host range cosmids are ideal for open resource metagenomics, accommodating large DNA inserts for screening or selections in multiple prokaryotic or eukaryotic hosts.
The Challenges of Functional Metagenomics
By capturing DNA extracted directly from environmental samples, the first metagenomic libraries were constructed in the late 1990s (Handelsman et al. 1998; Rondon et al. 2000). Metagenomic libraries are typically constructed for specific applications such as retrieving genes with desired functions. Functional metagenomics is the use of metagenomic libraries to isolate genes of interest based on associated activity of captured...
KeywordsBacterial Artificial Chromosome Broad Host Range Metagenomic Library Functional Metagenomics Metagenomic Sequence Data
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