Encyclopedia of Metagenomics

Living Edition
| Editors: Karen E. Nelson

Activated Sludge Metagenomics

  • Ke YuEmail author
  • Tong Zhang
Living reference work entry
DOI: https://doi.org/10.1007/978-1-4614-6418-1_717-3




Activated sludge metagenomics is the study of metagenome using genetic materials that extracted from activated sludge (AS).

Activated sludge is a widely applied effective biological process in wastewater treatment plants (WWTP) to eliminate the adverse environmental impacts (including toxicity, oxygen depletion, and algal blooms) caused by pollutants in the effluent from WWTP. It is well known that activated sludge is a dynamic and phylogenetically diverse microbial community (Zhang et al. 2012). Similar to soil and sediment, the activated sludge floc is highly complex, containing eukaryotes, bacteria, archaea, and viruses, in which bacteria are dominant and play important roles in removal of organic pollutants and nutrients (nitrogen and phosphorus) (for more information, please seeEnvironmental Genomics”). For revealing of microbial community and functions of AS, many molecular technologies, including metagenomics, have been utilized. In addition to...


Activate Sludge Metagenomic Library Metagenomic Analysis Enhance Biological Phosphorus Removal Metagenomic Study 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
This is a preview of subscription content, log in to check access.


  1. Albertsen M, Hansen LBS, Saunders AM, Nielsen PH, Nielsen KL. A metagenome of a full-scale microbial community carrying out enhanced biological phosphorus removal. ISME J. 2012;6(6):1094–106.PubMedCentralPubMedCrossRefGoogle Scholar
  2. He SM, Kunin V, Haynes M, Martin HG, Ivanova N, Rohwer F, Hugenholtz P, McMahon KD. Metatranscriptomic array analysis of ‘Candidatus Accumulibacter phosphatis’-enriched enhanced biological phosphorus removal sludge. Environ Microbiol. 2010;12(5):1205–17.PubMedCrossRefGoogle Scholar
  3. Li JG, Zhang KG, Han WJ. Cloning and biochemical characterization of a novel lipolytic gene from activated sludge metagenome, and its gene product. Microb Cell Fact. 2010;9:83.CrossRefGoogle Scholar
  4. Liaw RB, Cheng MP, Wu MC, Lee CY. Use of metagenomic approaches to isolate lipolytic genes from activated sludge. Bioresour Technol. 2010;101(21):8323–9.PubMedCrossRefGoogle Scholar
  5. Martin HG, Ivanova N, Kunin V, Warnecke F, Barry KW, McHardy AC, Yeates C, He SM, Salamov AA, Szeto E, Dalin E, Putnam NH, Shapiro HJ, Pangilinan JL, Rigoutsos I, Kyrpides NC, Blackall LL, McMahon KD, Hugenholtz P. Metagenomic analysis of two enhanced biological phosphorus removal (EBPR) sludge communities. Nat Biotechnol. 2006;24(10):1263–9.CrossRefGoogle Scholar
  6. Parsley LC, Consuegra EJ, Kakirde KS, Land AM, Harper WF, Liles MR. Identification of diverse antimicrobial resistance determinants carried on bacterial, plasmid, or viral metagenomes from an activated sludge microbial assemblage. Appl Environ Microbiol. 2010;76(11):3753–7.PubMedCentralPubMedCrossRefGoogle Scholar
  7. Sanapareddy N, Hamp TJ, Gonzalez LC, Hilger HA, Fodor AA, Clinton SM, et al. Molecular diversity of a North Carolina wastewater treatment plant as revealed by pyrosequencing. Appl Environ Microbiol. 2009;75(6):1688–96.PubMedCentralPubMedCrossRefGoogle Scholar
  8. Streit WR, Schmitz RA. Metagenomics - the key to the uncultured microbes. Curr Opin Microbiol. 2004;7(5):492–8.PubMedCrossRefGoogle Scholar
  9. Wexler M, Bond PL, Richardson DJ, Johnston AWB. A wide host-range metagenomic library from a waste water treatment plant yields a novel alcohol/aldehyde dehydrogenase. Environ Microbiol. 2005;7(12):1917–26.PubMedCrossRefGoogle Scholar
  10. Yu K, Zhang T. Metagenomic and metatranscriptomic analysis of microbial community structure and gene expression of activated sludge. PLoS One. 2012;7(5).Google Scholar
  11. Zhang T, Han WJ, Liu ZP. Gene cloning and characterization of a novel esterase from activated sludge metagenome. Microb Cell Fact. 2009;9:25 (2010;8:67).CrossRefGoogle Scholar
  12. Zhang T, Shao MF, Ye L. 454 Pyrosequencing reveals bacterial diversity of activated sludge from 14 sewage treatment plants. ISME J. 2012;6(6):1137–47.PubMedCentralPubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  1. 1.Environmental Biotechnology LaboratoryThe University of Hong KongPokfulamHong Kong