CLUSEAN, the CLUster SEquence ANalyzer, is a BioPerl-based software pipeline for the annotation of secondary metabolite biosynthetic gene clusters encoding the biosynthesis of molecules with, e.g., antibiotic or anticancer activities. CLUSEAN contains modules for automated homology search, protein domain identification, and, in case of modular polyketide synthases and non-ribosomal peptide synthetases-containing pathways, substrate prediction for the biosynthetic enzymes.
A majority of antimicrobials used in human medicine to combat infectious diseases, e.g., tetracycline, penicillin, vancomycin, or erythromycin, many anticancer drugs, and other bioactive molecules, e.g., the immunosuppressant rapamycin, are derived from microbial secondary metabolites, also denoted as natural products. These compounds are mainly synthesized by bacteria and fungi. Traditionally, screening for such compounds is performed by isolating potential...
KeywordsBiosynthetic Gene Cluster Adenylation Domain Secondary Metabolite Gene Cluster Microbial Secondary Metabolite Secondary Metabolite Biosynthetic Gene Cluster
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