Bacteriocin Mining in Metagenomes
Bacteriocins are heat-stable ribosomally synthesized peptides produced by one bacterium which are active against other bacteria and against which the producer has a specific immunity mechanism.
Bacteriocins are ribosomally synthesized antimicrobial peptides that are produced by many bacteria and which kill or inhibit the growth of other bacteria. Bacteriocin producers are protected as a consequence of dedicated immunity (self-protective) systems (Cotter et al. 2005). Bacteriocins are of both academic and commercial interest, with several in use as food preservatives or as the active agent in clinical or veterinary antimicrobials. It is not surprising that there is significant interest in the identification and characterization of new bacteriocin gene clusters. The growing volume of metagenomic sequence data is an important resource which can be mined for the in silico discovery of novel bacteriocins.
A Background to Bacteriocins
KeywordsCysteine Serine Dehydration Microbe Arena
- Glass EM, Wilkening J, Wilke A, Antonopoulos D Meyer F Using the metagenomics RAST server (MG-RAST) for analyzing shotgun metagenomes. Cold Spring Harb Protoc. 2010(1), pdb prot5368, doi:2010/1/pdb.prot5368 [pii] 10.1101/pdb.prot5368.Google Scholar
- Majchrzykiewicz JA, Lubelski J, Moll GN, Kuipers A, Bijlsma JJ, Kuipers OP, et al. Production of a class II two-component lantibiotic of Streptococcus pneumoniae using the class I nisin synthetic machinery and leader sequence. Antimicrob Agents Chemother. 2010;54(4):1498–505. doi:10.1128/AAC.00883-09.PubMedCentralPubMedCrossRefGoogle Scholar
- Sun S, Chen J, Li W, Altintas I, Lin A, Peltier S, et al. Community cyberinfrastructure for advanced microbial ecology research and analysis: the CAMERA resource. Nucleic Acids Res. 2011;39(Database issue):D546-551. doi:10.1093/nar/gkq1102.Google Scholar