Novel Alkalistable and Thermostable Xylanase-Encoding Gene (Mxyl) Retrieved from Compost-Soil Metagenome
For retrieving genes encoding thermo-alkali-stable xylanases by culture-independent (metagenomic) approach, the DNA extracted from hot and alkaline environmental samples is restricted and the fragments are cloned. The clones are screened to select colonies with the desired xylanase gene, and the insert is sequenced and the gene is subcloned and expressed. The recombinant xylanase is purified and characterized and tested for its applicability in generating xylo-oligosaccharides from agro-residues and pulp bleaching.
Hemicellulosic components are integral part of lignocellulosic residues and the second most abundant renewable polymer of plant cell walls after cellulose. Xylan is the main constituent in hemicelluloses of lignocellulosic agro-residues. β-1,4-linked xylosyl residues form the backbone of xylan...
KeywordsSugarcane Bagasse Xylanase Activity Metagenomic Library Beech Wood Ramie Fiber
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