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Novel Alkalistable and Thermostable Xylanase-Encoding Gene (Mxyl) Retrieved from Compost-Soil Metagenome

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Encyclopedia of Metagenomics

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Synonyms

Community genomics; Culture-independent approach; Environmental genomics; Endoxylanase; Endo-β-1,4 xylanase; Thermo-alkali-stable xylanase; Xylanase

Definition

For retrieving genes encoding thermo-alkali-stable xylanases by culture-independent (metagenomic) approach, the DNA extracted from hot and alkaline environmental samples is restricted and the fragments are cloned. The clones are screened to select colonies with the desired xylanase gene, and the insert is sequenced and the gene is subcloned and expressed. The recombinant xylanase is purified and characterized and tested for its applicability in generating xylo-oligosaccharides from agro-residues and pulp bleaching.

Introduction

Hemicellulosic components are integral part of lignocellulosic residues and the second most abundant renewable polymer of plant cell walls after cellulose. Xylan is the main constituent in hemicelluloses of lignocellulosic agro-residues. β-1,4-linked xylosyl residues form the backbone of xylan...

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Authors and Affiliations

  1. Department of Microbiology, University of Delhi, Benito Juarez Road, 110021, New Delhi, India

    Digvijay Verma & Tulasi Satyanarayana

Authors
  1. Digvijay Verma
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  2. Tulasi Satyanarayana
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Corresponding author

Correspondence to Tulasi Satyanarayana .

Editor information

Editors and Affiliations

  1. J. Craig Venter Institute (JCVI), Rockville, Maryland, USA

    Karen E. Nelson

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Verma, D., Satyanarayana, T. (2014). Novel Alkalistable and Thermostable Xylanase-Encoding Gene (Mxyl) Retrieved from Compost-Soil Metagenome. In: Nelson, K. (eds) Encyclopedia of Metagenomics. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-6418-1_413-2

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  • DOI: https://doi.org/10.1007/978-1-4614-6418-1_413-2

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  • Online ISBN: 978-1-4614-6418-1

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