Instrumentation for Molecular Testing
The following is a brief overview of representative instruments and categories of instruments central to the practice of molecular diagnostics and related fields. Some of these instruments or technologies have been introduced in other chapters. The goal in this chapter is to provide the readers with latest and common instrument and technologies with basic understanding of the principle of operation and the clinical applications. While a comprehensive survey of all such equipment is beyond the scope of this handbook, more extensive information is readily available from manufacturers’ websites and brochures.
KeywordsPolymerase Chain Reaction Light Emit Diode General Information Fluorescent Resonance Energy Transfer Conventional Polymerase Chain Reaction
- C & L Instruments Inc., Tutorial – fluorescent microscope optics. C&L Instruments, Inc. Hershey, PA. http://www.fluorescence.com/tutorial/fm-optic.htm.
- Kohane IS, Kho AT, Butte AJ. Microarrays for an integrative genomics. Cambridge, MA: MIT Press; 2003.Google Scholar
- National Center for Biotechnology Information (NCBI) http://www.ncbi.nlm.nih.gov/About/primer/microarrays.html.
- Pieprzyk M, High H. Fluidigm dynamic arrays provide a platform for single-cell gene expression analysis. Nat Meth. 2009;6. http://www.nature.com/nmeth/journal/v6/n7/full/nmeth.f.259.html.