Encyclopedia of Metalloproteins

2013 Edition
| Editors: Robert H. Kretsinger, Vladimir N. Uversky, Eugene A. Permyakov

Biarsenical Fluorescent Probes

Reference work entry
DOI: https://doi.org/10.1007/978-1-4614-1533-6_440

Synonyms

Definition

Fluorescent biarsenical probes refer to chemical agents that are built on fluorescent platforms with two proton to arsenic substitutions at certain positions that are capped with two molecules of 1,2-ethanedithiol. The probes remain nonluminescent until protein conjugation when they became highly fluorescent. Due to stability of arsenic-sulfur bond, biarsenical probes have very high affinity to four cysteine residues, which may be genetically introduced to proteins as a short sequence appropriately spaced (e.g., CCPGCC) or by incorporation into secondary structure elements.

Principles of the Biarsenical Labeling Strategy

Many of the chemistry-driven strategies used for monitoring, controlling, and modifying protein functions require small fluorescent probes that are selectively attached to the protein of interest. The probe conjugation is mostly achieved by single or multiple...
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References

  1. Adams SR, Tsien RY (2008) Preparation of the membrane-permeant biarsenicals FlAsH-EDT2 and ReAsH-EDT2 for fluorescent labeling of tetracysteine-tagged proteins. Nat Protoc 3:1527–1534PubMedCrossRefGoogle Scholar
  2. Crivat G, Tarasaka JW (2012) Imaging proteins inside cells with fluorescent tags. Trends Biotechnol 30:8–16PubMedCrossRefGoogle Scholar
  3. Hermanson GT (2008) Bioconjugate techniques. Academic, AmsterdamGoogle Scholar
  4. Hoffmann C, Gaietta G, Zürn A, Adams SR, Terrillon S, Ellisman MH, Tsien RY, Lohse MJ (2010) Fluorescent labeling of tetracysteine-tagged proteins in intact cells. Nat Protoc 5:1666–1677PubMedCrossRefGoogle Scholar
  5. Jakobs S, Andresen M, Wurm CA (2008) “FlAsH” protein labeling. In: Miller LW (ed) Probes and tags to study biomolecular function: for proteins, RNA, and membranes. Wiley-VCH, Weinheim, pp 73–88CrossRefGoogle Scholar
  6. Pomorski A, Krężel A (2011) Exploration of biarsenical chemistry – challenges in protein research. Chembiochem 12:1152–1167PubMedCrossRefGoogle Scholar
  7. Scheck RA, Schepartz A (2011) Surveying protein structure and function using bis-arsenical small molecules. Acc Chem Res 44:654–665PubMedCrossRefGoogle Scholar
  8. Tsien RY (2005) Building and breeding molecules to spy on cells and tumors. FEBS Lett 579:927–932PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  1. 1.Department of Protein Engineering, Faculty of BiotechnologyUniversity of WrocławWrocławPoland