Encyclopedia of Metalloproteins

2013 Edition
| Editors: Robert H. Kretsinger, Vladimir N. Uversky, Eugene A. Permyakov

Biarsenical Fluorescent Probes

Reference work entry
DOI: https://doi.org/10.1007/978-1-4614-1533-6_440



Fluorescent biarsenical probes refer to chemical agents that are built on fluorescent platforms with two proton to arsenic substitutions at certain positions that are capped with two molecules of 1,2-ethanedithiol. The probes remain nonluminescent until protein conjugation when they became highly fluorescent. Due to stability of arsenic-sulfur bond, biarsenical probes have very high affinity to four cysteine residues, which may be genetically introduced to proteins as a short sequence appropriately spaced (e.g., CCPGCC) or by incorporation into secondary structure elements.

Principles of the Biarsenical Labeling Strategy

Many of the chemistry-driven strategies used for monitoring, controlling, and modifying protein functions require small fluorescent probes that are selectively attached to the protein of interest. The probe conjugation is mostly achieved by single or multiple...
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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  1. 1.Department of Protein Engineering, Faculty of BiotechnologyUniversity of WrocławWrocławPoland