Encyclopedia of Metalloproteins

2013 Edition
| Editors: Robert H. Kretsinger, Vladimir N. Uversky, Eugene A. Permyakov

Bismuth, Interaction with Gastrin

Reference work entry
DOI: https://doi.org/10.1007/978-1-4614-1533-6_200004

Definition

The peptide hormone gastrin was originally identified as a stimulant of acid secretion, but is now known to also act as a growth factor in the gastrointestinal tract (Dockray et al. 2001). Gastrin is synthesized as a precursor of 101 amino acids (preprogastrin) which, on removal of the signal peptide of 21 amino acids, yields progastrin (80 amino acids). Proteolytic processing in antral G cells in the stomach generates a number of intermediate peptides, including glycine-extended gastrin17 (Ggly), which has the sequence ZGPWLEEEEEAYGWMDFG. Transamidation of the C-terminal glycine yields the C-terminal amidated phenylalanine characteristic of amidated gastrin (Gamide). Both Ggly and Gamide are independently active, via different receptors, in the gastrointestinal tract.

Basic Characteristics

Iron: Interaction with Gastrin

Fluorescence experiments have revealed that both Ggly and Gamide bind two ferric ions with high affinity (Kd= 0.6 μM, pH 4.0) in aqueous solution (Baldwin...

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References

  1. Aly A, Shulkes A, Baldwin GS (2004) Gastrins, cholecystokinins and gastrointestinal cancer. Biochim Biophys Acta 1704:1–10PubMedGoogle Scholar
  2. Baldwin GS, Curtain CC, Sawyer WH (2001) Selective, high-affinity binding of ferric ions by glycine-extended gastrin(17). Biochemistry 40:10741–10746PubMedCrossRefGoogle Scholar
  3. Baldwin GS (2004) Properties of the complex between recombinant human progastrin and ferric ions. The Protein Journal 23:65–70PubMedCrossRefGoogle Scholar
  4. Chen D, Zhao CM, Dockray GJ et al (2000) Glycine-extended gastrin synergizes with gastrin 17 to stimulate acid secretion in gastrin-deficient mice. Gastroenterology 119:756–765PubMedCrossRefGoogle Scholar
  5. Dockray GJ, Varro A, Dimaline R et al (2001) The gastrins: their production and biological activities. Annu Rev Physiol 63:119–139PubMedCrossRefGoogle Scholar
  6. Ferrand A, Lachal S, Bramante G et al (2010) Stimulation of proliferation in the colorectal mucosa by gastrin precursors is blocked by desferrioxamine. Am J Physiol Gastrointestinal and Liver Physiology 299:G220–G227CrossRefGoogle Scholar
  7. Gorbach SL (1990) Bismuth therapy in gastrointestinal diseases. Gastroenterology 99:863–875PubMedGoogle Scholar
  8. He H, Shehan BP, Barnham KJ et al (2004) Biological activity and ferric ion binding of fragments of glycine-extended gastrin. Biochemistry 43:11853–11861PubMedCrossRefGoogle Scholar
  9. Houben MH, van de Beek D, Hensen EF et al (1999) A systematic review of Helicobacter pylori eradication therapy–the impact of antimicrobial resistance on eradication rates. Aliment Pharmacol Ther 13:1047–1055PubMedCrossRefGoogle Scholar
  10. Pannequin J, Barnham KJ, Hollande F et al (2002) Ferric ions are essential for the biological activity of the hormone glycine-extended gastrin. J Biol Chem 277:48602–48609PubMedCrossRefGoogle Scholar
  11. Pannequin J, Tantiongco JP, Kovac S et al (2004a) Divergent roles for ferric ions in the biological activity of amidated and non-amidated gastrins. J Endocrinol 181:315–325PubMedCrossRefGoogle Scholar
  12. Pannequin J, Kovac S, Tantiongco JP et al (2004b) A novel effect of bismuth ions: selective inhibition of the biological activity of glycine-extended gastrin. J Biol Chem 279:2453–2460PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  1. 1.Department of Surgery, Austin HealthThe University of MelbourneHeidelbergAustralia