In 1989, P. Philipopov’s group from M.V. Lomonosov Moscow State University invented a method for purification of the visual G-protein transducin (Gt) and some other G-proteins. The idea of the method was based on the ability of visual rhodopsin to bind and to release transducin in the absence and in the presence of GTP, respectively. For this aim, a column with delipidated visual rhodopsin immobilized on Concanavalin A Sepharose was used. Chromatography of a crude extract of bovine rod outer segments on the column allowed one to obtain a set of transducin subunits with a slight contamination of cGMP-phosphodiesterase. Also, an admixture of an unknown protein with an apparent molecular weight of 26 K could be seen on the electrophoregram. The unknown protein attracted the attention of the group since the capability of...
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