Historical Background
In 1989, P. Philipopov’s group from M.V. Lomonosov Moscow State University invented a method for purification of the visual G-protein transducin (Gt) and some other G-proteins. The idea of the method was based on the ability of visual rhodopsin to bind and to release transducin in the absence and in the presence of GTP, respectively. For this aim, a column with delipidated visual rhodopsin immobilized on Concanavalin A Sepharose was used. Chromatography of a crude extract of bovine rod outer segments on the column allowed one to obtain a set of transducin subunits with a slight contamination of cGMP-phosphodiesterase. Also, an admixture of an unknown protein with an apparent molecular weight of 26 K could be seen on the electrophoregram. The unknown protein attracted the attention of the group since the capability of binding to...
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References
Adamus G. The role of recoverin in autoimmunity. In: Philippov PP, Koch KW, editors. Neuronal calcium sensor proteins. New York: Nova; 2006. p. 181–99.
Ames JB, Ishima R, Tanaka T, Gordon JI, Stryer L, Ikura M. Molecular mechanics of calcium-myristoyl switches. Nature. 1997;389:198–202.
Ames JB, Levay K, Wingard JN, Lusin JD, Slepak VZ. Structural basis for calcium-induced inhibition of rhodopsin kinase by recoverin. J Biol Chem. 2006;281:37237–45.
Bazhin AV, Savchenko MS, Shifrina ON, Demoura SA, Chikina SY, Jaques G, Kogan EA, Chuchalin AG, Philippov PP. Recoverin as a paraneoplastic antigen in lung cancer: the occurrence of anti-recoverin autoantibodies in sera and recoverin in tumors. Lung Cancer. 2004;44:193–8.
Bazhin AV, Schadendorf D, Willner N, De Smet C, Heinzelmann A, Tikhomirova NK, Umansky V, Philippov PP, Eichmüller SB. Photoreceptor proteins as cancer-retina antigens. Int J Cancer. 2007;120:1268–76.
Burgoyne RD, Weiss JL. The neuronal calcium sensor family of Ca2+-binding proteins. Biochem J. 2001;353:1–12.
Calvez P, Demers E, Boisselier E, Salesse C. Analysis of the contribution of saturated and polyunsaturated phospholipid monolayers to the binding of proteins. Langmuir. 2011;27:1373–9.
Chen CK, Woodruff ML, Chen FS, Chen D, Fain GL. Background light produces a recoverin-dependent modulation of activated-rhodopsin lifetime in mouse rods. J Neurosci. 2010;30:1213–20.
Förster JR, Lochnit G, Stöhr H. Proteomic analysis of the membrane palmitoylated protein-4 (MPP4)-associated protein complex in the retina. Exp Eye Res. 2009;88:39–48.
Fries R, Reddy PP, Mikhaylova M, Haverkamp S, Wei T, Müller M, Kreutz MR, Koch K-W. Dynamic cellular translocation of caldendrin is facilitated by the Ca2+-myristoyl switch of recoverin. J Neurochem. 2010;113:1150–62.
Higgins MK, Oprian DD, Schertler GF. Recoverin binds exclusively to an amphipathic peptide at the N terminus of rhodopsin kinase, inhibiting rhodopsin phosphorylation without affecting catalytic activity of the kinase. J Biol Chem. 2006;281:19426–32.
Permyakov SE, Cherskaya AM, Wasserman LA, Khokhlova TI, Senin II, Zargarov AA, Zinchenko DV, Zernii EY, Lipkin VM, Philippov PP, Uversky VN, Permyakov EA. Recoverin is a zinc-binding protein. J Proteome Res. 2003;2:51–7.
Permyakov SE, Nazipova AA, Denesyuk AI, Bakunts AG, Zinchenko DV, Lipkin VM, Uversky VN, Permyakov EA. Recoverin as a redox-sensitive protein. J Proteome Res. 2007;6:1855–63.
Philippov PP, Senin II, Koch K-W. Recoverin: a calcium-dependent regulator of the visual transduction. In: Philippov PP, Koch KW, editors. Neuronal calcium sensor proteins. New York: Nova; 2006. p. 139–51.
Sampath AP, Strissel KJ, Elias R, Arshavsky VY, McGinnis JF, Chen J, Kawamura S, Rieke F, Hurley JB. Recoverin improves rod-mediated vision by enhancing signal transmission in the mouse retina. Neuron. 2005;46:413–20.
Senin II, Koch KW, Akhtar M, Philippov PP. Ca2+-dependent control of rhodopsin phosphorylation: recoverin and rhodopsin kinase. Adv Exp Med Biol. 2002;514:69–99.
Senin II, Churumova VA, Philippov PP, Koch K-W. Membrane binding of the neuronal calcium sensor recoverin – modulatory role of the charged carboxy-terminus. BMC Biochem. 2007;8:24.
Strissel KJ, Lishko PV, Trieu LH, Kennedy MJ, Hurley JB, Arshavsky VY. Recoverin undergoes light-dependent intracellular translocation in rod photoreceptors. J Biol Chem. 2005;280:29250–5.
Tanaka T, Ames JB, Harvey TS, Stryer L, Ikura M. Sequestration of the membrane-targeting myristoyl group of recoverin in the calcium-free state. Nature. 1995;376:444–7.
Valentine KG, Mesleh MF, Opella SJ, Ikura M, Ames JB. Structure, topology, and dynamics of myristoylated recoverin bound to phospholipid bilayers. Biochemistry. 2003;42:6333–40.
Weiergräber OH, Senin II, Zernii EY, Churumova VA, Kovaleva NA, Nazipova AA, Permyakov SE, Permyakov EA, Philippov PP, Granzin J, Koch K-W. Tuning of a neuronal calcium sensor. J Biol Chem. 2006;281:37594–602.
Zernii EY, Komolov KE, Permyakov SE, Kolpakova T, Dell'orco D, Poetzsch A, Knyazeva EL, Grigoriev II, Permyakov EA, Senin II, Philippov PP, Koch KW. Involvement of the recoverin C-terminal segment in recognition of the target enzyme rhodopsin kinase. Biochem J. 2011;435:441–50.
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Philippov, P.P., Zernii, E.Y. (2012). Recoverin. In: Choi, S. (eds) Encyclopedia of Signaling Molecules. Springer, New York, NY. https://doi.org/10.1007/978-1-4419-0461-4_606
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DOI: https://doi.org/10.1007/978-1-4419-0461-4_606
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