Encyclopedia of Signaling Molecules

2012 Edition
| Editors: Sangdun Choi

Ect2 (Epithelial Cell Transforming 2 Oncogene)

Reference work entry
DOI: https://doi.org/10.1007/978-1-4419-0461-4_493

Synonyms

Historical Background

Ect2 is a guanine nucleotide exchange factor (GEF) of the Rho GTPases (Tatsumoto et al. 1999). It was isolated as a cDNA clone with capability of converting mouse fibroblasts to malignantly transformed cells (Miki et al. 1991, 1993). Transforming Ect2 variants lacked the N-terminal regulatory domain, and efficiently induced focus formation, anchorage-independent cell growth, and cell invasiveness in mouse fibroblasts. Injection of athymic nude mice with Ect2 transformants resulted in tumor formation. While full-length Ect2 is localized only in the nucleus, its oncogenic variants are detected in the cytoplasm as well. In M phase, Ect2 distributes in the entire cells after nuclear membrane breakdown, and is concentrated on mitotic spindles and then central spindles. In the last stage of M phase, Ect2 translocates to the midbody, which is formed between two emerging daughter cells. Perturbation of Ect2 function in M...

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References

  1. Hara T, Abe M, Inoue H, Yu LR, Veenstra TD, Kang YH, et al. Cytokinesis regulator ECT2 changes its conformation through phosphorylation at Thr-341 in G2/M phase. Oncogene. 2006;25(4):566–78.PubMedGoogle Scholar
  2. Justilien V, Fields AP. Ect2 links the PKCiota-Par6alpha complex to Rac1 activation and cellular transformation. Oncogene. 2009;28(41):3597–607.PubMedCrossRefGoogle Scholar
  3. Kamijo K, Ohara N, Abe M, Uchimura T, Hosoya H, Lee JS, et al. Dissecting the role of Rho-mediated signaling in contractile ring formation. Mol Biol Cell. 2006;17(1):43–55.PubMedCrossRefGoogle Scholar
  4. Liu XF, Ishida H, Raziuddin R, Miki T. Nucleotide exchange factor ECT2 interacts with the polarity protein complex Par6/Par3/protein kinase Czeta (PKCzeta) and regulates PKCzeta activity. Mol Cell Biol. 2004;24(15):6665–75.PubMedCrossRefGoogle Scholar
  5. Liu XF, Ohno S, Miki T. Nucleotide exchange factor ECT2 regulates epithelial cell polarity. Cell Signal. 2006;18(10):1604–15.PubMedCrossRefGoogle Scholar
  6. Miki T. Involvement of Rho GTPases in cell division. In: Miki T, editor. Signal transduction of cell division. Kerala: Research Signpost; 2005. p. 263–84.Google Scholar
  7. Miki T, Fleming TP, Bottaro DP, Rubin JS, Ron D, Aaronson SA. Expression cDNA cloning of the KGF receptor by creation of a transforming autocrine loop. Science. 1991;251(4989):72–5.PubMedCrossRefGoogle Scholar
  8. Miki T, Smith C, Long J, Eva A, Fleming T. Oncogene ect2 is related to regulators of small GTP-binding proteins. Nature. 1993;362(6419):462–5.PubMedCrossRefGoogle Scholar
  9. Miki T, Hara T, Kamijo K, Mahoney T, Lee J-S. Rho GTPases and cell division. In: Pandalai S, editor. Recent research developments in biological chemistry. Kerala: Research Signpost; 2002. p. 255–72.Google Scholar
  10. Miki T, Kim H, Senju Y.: Ect2. http://www.signaling-gateway.org/molecule/query?afcsid=A000812 (2009), ID: A000812
  11. Minoshima Y, Kawashima T, Hirose K, Tonozuka Y, Kawajiri A, Bao YC, et al. Phosphorylation by Aurora B converts MgcRacGAP to a RhoGAP during cytokinesis. Dev Cell. 2003;4(4):549–60.PubMedCrossRefGoogle Scholar
  12. Niiya F, Tatsumoto T, Lee KS, Miki T. Phosphorylation of the cytokinesis regulator ECT2 at G2/M phase stimulates association of the mitotic kinase Plk1 and accumulation of GTP-bound RhoA. Oncogene. 2006;25(6):827–37.PubMedCrossRefGoogle Scholar
  13. Oceguera-Yanez F, Kimura K, Yasuda S, Higashida C, Kitamura T, Hiraoka Y, et al. Ect2 and MgcRacGAP regulate the activation and function of Cdc42 in mitosis. J Cell Biol. 2005;168(2):221–32.PubMedCrossRefGoogle Scholar
  14. Prokopenko SN, Saint R, Bellen HJ. Untying the Gordian knot of cytokinesis. Role of small G proteins and their regulators. J Cell Biol. 2000;148(5):843–8.PubMedCrossRefGoogle Scholar
  15. Saito S, Liu XF, Kamijo K, Raziuddin R, Tatsumoto T, Okamoto I, et al. Deregulation and mislocalization of the cytokinesis regulator ECT2 activate the Rho signaling pathways leading to malignant transformation. J Biol Chem. 2004;279(8):7169–79.PubMedCrossRefGoogle Scholar
  16. Sano M, Genkai N, Yajima N, Tsuchiya N, Homma J, Tanaka R, et al. Expression level of ECT2 proto-oncogene correlates with prognosis in glioma patients. Oncol Rep. 2006;16(5):1093–8.PubMedGoogle Scholar
  17. Tatsumoto T, Xie X, Blumenthal R, Okamoto I, Miki T. Human ECT2 is an exchange factor for Rho GTPases, phosphorylated in G2/M phases, and involved in cytokinesis. J Cell Biol. 1999;147(5):921–7.PubMedCrossRefGoogle Scholar
  18. Wolfe BA, Takaki T, Petronczki M, Glotzer M. Polo-like kinase 1 directs assembly of the HsCyk-4 RhoGAP/Ect2 RhoGEF complex to initiate cleavage furrow formation. PLoS Biol. 2009;7(5):e1000110.PubMedCrossRefGoogle Scholar
  19. Yuce O, Piekny A, Glotzer M. An ECT2-centralspindlin complex regulates the localization and function of RhoA. J Cell Biol. 2005;170(4):571–82.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Laboratory of Cellular SignalingNagaoka University of TechnologyNiigataJapan