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Fluorescence In Situ Hybridization (FISH)

  • Reference work entry
Encyclopedia of Geobiology

Part of the book series: Encyclopedia of Earth Sciences Series ((EESS))

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Definition

FISH (fluorescence in situ hybridization) is a hybridization technique employing reverse complementary fluorescently labeled probes to detect and localize only those parts of the chromosome, a gene or its transcript with which they show a high degree of sequence similarity. Results are mostly evaluated by either fluorescence microscopy or cell sorting methods like flow cytometry. Depending on target gene, probe chemistry, labeling, and combination with other analytical tools, a wide range of different FISH applications can be employed for advanced in situ analysis of community structure, dynamics, localization, activity, function and interactions of different cellular species.

Introduction

Antonie van Leeuwenhoek’s ingenious improvement of the microscope in the seventeenth century revolutionized biology as this initiated novel ways to explore our world and ultimately led to the discovery of a new dimension of life forms, the microorganisms. However, since traditional...

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Authors and Affiliations

  1. Department of Microbiology Technical University of Munich Emil-Ramann-Str. 4, 85354, Freising-Weihenstephan, Germany

    Dr. Natuschka M. Lee

  2. Department of Microbiology, Technische Universität München, 85354, Freising, Germany

    Daniela B. Meisinger

  3. Department Microbe-Plant Interactions, Helmholtz Zentrum München, German Research Center for Environmental Health (GmbH), 85764, Neuherberg, Germany

    Dr. Michael Schmid

  4. Research scientist Department Microbe-Plant Interactions, Helmholtz Zentrum München German Research Center for Environmental Health (GmbH), D-85764, Neuherberg, Germany

    Dr. Michael Rothballer

  5. Department of Microbiology, University of Tennessee, M409 WLS Bldg., 1414 Cumberland Avenue, Knoxville, TN, USA, 37996-0845

    Dr. Frank E. Löffler

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Lee, N.M., Meisinger, D.B., Schmid, M., Rothballer, M., Löffler, F.E. (2011). Fluorescence In Situ Hybridization (FISH). In: Reitner, J., Thiel, V. (eds) Encyclopedia of Geobiology. Encyclopedia of Earth Sciences Series. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-9212-1_91

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