Encyclopedia of Entomology

2008 Edition
| Editors: John L. Capinera

Molecular Diagnosis

  • Maria Navajas
  • George Roderick
Reference work entry
DOI: https://doi.org/10.1007/978-1-4020-6359-6_4659

Molecular diagnosis refers to the taxonomic determination of organisms based on molecular criteria. Correct taxonomic determination is critical for entomological applications in many fields, including agriculture, environmental science, biosecurity, and human health. Because a major challenge in taxonomy is the discrimination among closely related forms, diagnosis mainly concerns the identification of organisms at the species level. One example is the case of cryptic species complexes, which are groups of closely related species that are difficult or impossible to distinguish by morphological traits alone. These complexes are known from a wide variety of arthropods and have been well studied in medically and economically important insects. For example, despite a near identity in morphological appearance, several sibling species of mosquitoes are separable with a variety of molecular-based methods. Conversely, molecular systems can be used to establish synonymy between presumed cryptic...

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References

  1. Avise JC (2004) Molecular markers, natural history and evolution, 2nd ed. Chapman and Hall, New YorkGoogle Scholar
  2. Behura SK (2006) Molecular marker systems in insects: current trends and future avenues. Mol Ecol 15:3087–3113PubMedCrossRefGoogle Scholar
  3. Bickford D, Lohman DJ, Sodhi NS, Ng PKL, Meier R, Winkler K, Ingram KK, Das I (2007) Cryptic species as a window on diversity and conservation. Trends Ecol Evol 22:148–155PubMedCrossRefGoogle Scholar
  4. Caterino MS, Cho S, Sperling FAH (2000) The current state of insect molecular systematics: a thriving tower of Babel. Annu Rev Entomol 45:1–54PubMedCrossRefGoogle Scholar
  5. Clarke AR, Armstrong KF, Carmichael AE, Milne JR, Raghu S, Roderick GK, Yeates DK (2005) Invasive phytophagous pests arising through a recent tropical evolutionary radiation: the Bactrocera dorsalis. complex of fruit flies. Annu Rev Entomol 50:293–319PubMedCrossRefGoogle Scholar
  6. Excoffier L, Heckel G (2006) Computer programs for population genetics data analysis: a survival guide. Nat Rev Genet 7:745–758PubMedCrossRefGoogle Scholar
  7. Gomez-Zurita J, Galian J (2005) Current knowledge on genes and genomes of phytophagous beetles (Coleoptera: Chrysomeloidea, Curculionoidea): a review. Eur J Entomol 102:577–597Google Scholar
  8. Gotoh T, Gutierrez J, Navajas M (1998) Molecular comparison of the sibling species Tetranychus pueraricola. Ehara & Gotoh and T. urticae Koch (Acari: Tetranychidae). Entomol Sci 1:55–57Google Scholar
  9. Krzywinski J, Besansky NJ (2003) Molecular systematics of Anopheles.: from subgenera to subpopulations. Annu Rev Entomol 48:111–139PubMedCrossRefGoogle Scholar
  10. Luikart G, England PR, Tallmon D, Jordan S, Taberlet P (2003) The power and promise of population genomics: from genotyping to genome typing. Nat Rev Genet 4:981–994PubMedCrossRefGoogle Scholar
  11. Manelm S, Gaggiotti OE, Waples RS (2005) Assignment methods: matching biological questions with appropriate techniques. Trends Ecol Evol 20:136–142CrossRefGoogle Scholar
  12. Navajas M, Boursot P (2003) Nuclear ribosomal DNA monophyly versus. mitochondrial DNA polyphyly in two closely related mite species: the influence of life history and molecular drive. Proc R Soc Lond B (Suppl) 270:S124–S127CrossRefGoogle Scholar
  13. Navajas M, Gutierrez J, Williams M, Gotoh T (2001) Synonymy between two spider mite species, Tetranychus kanzawai. and T. hydrangea, shown by ribosomal ITS2 sequences and cross-breeding. Bull Entomol Res 91:117–123PubMedGoogle Scholar
  14. Noor MAF, Feder JL (2006) Speciation genetics: evolving approaches. Nat Rev Genet 7:851–861PubMedCrossRefGoogle Scholar
  15. Robinson GE, Evans JD, Maleszka R, Robertson HM, Weaver DB, Worley K, Gibbs RA, Weinstock GM (2006) Sweetness and light: illuminating the honey bee genome. Insect Mol Biol 15:535–539PubMedCrossRefGoogle Scholar
  16. Rosenberg NA, Nordborg M (2002) Genealogical trees, coalescent theory and the analysis of genetic polymorphisms. Nat Genet 3:380–390Google Scholar
  17. Rugman-Jones PF, Hoddle MS, Mound LA, Stouthamer R (2006) Molecular identification key for pest species of Scirtothrips. (Thysanoptera: Thripidae). J Econ Entomol 99: 1813–1819PubMedCrossRefGoogle Scholar
  18. Savolainen V, Cowan RS, Vogler AP, Roderick GK, Lane R (2005) Towards writing the encyclopaedia of life: an introduction to DNA barcoding. Philos Trans R Soc Biol Sci 360:1805–1811CrossRefGoogle Scholar
  19. Schlötterer C (2004) The evolution of molecular markers–just a matter of fashion? Nat Rev Genet 5:63–69PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Maria Navajas
    • 1
  • George Roderick
    • 2
  1. 1.INRA, UMR CBGP (INRA/IRD/Cirad/ Montpellier SupAgro)Montferrier-sur-LezFrance
  2. 2.University of CaliforniaBerkeleyUSA