Genetic Modification of Drosophila by P Elements
It became possible to genetically modify Drosophila melanogaster using recombinant DNA methods in 1982 when P elements (described below) were identified and genetically altered to serve as mechanisms (vectors) for inserting genes into the nuclear genome of Drosophila. The use of P-element vectors provides a powerful tool that has modernized Drosophila genetics and made it possible to study the role of many genes important in development and behavior. In addition, the P-element system has served as a model for scientists wanting to genetically modify other insects for use in pest management programs ( Transgenic Insects).
P Elements are Disposable Elements
P elements are transposable (movable) elements that have been harnessed as a tool for genetically modifying the fruitfly Drosophila melanogaster in a consistent manner. Transposable elements are independent genetic elements that can move within and between genomes; some call them selfish genetic elements. The development of P...
- Ashburner M (1989) Drosophila. A laboratory handbook. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New YorkGoogle Scholar
- Fujioka MJ, Jaynes B, Bejsovec A, Weir M (2000) Production of transgenic Drosophila. In: Tuan RS, Lo CW (eds) Methods in molecular biology, vol 126: Developmental biology protocols, vol 2. Humana Press, Totowa, NJ, pp 353–363Google Scholar
- Hoy MA (2003) Insect molecular genetics, 2nd edn. Academic Press, San Diego, CAGoogle Scholar
- Karess RE (1987) P element mediated germline transformation of Drosophila. In: Glover DM (ed) DNA cloning, vol 2. A practical approach. IRL Press, Oxford, UK, pp 121–141Google Scholar
- Roberts DB (ed) (1986) Drosophila. A practical approach. IRL Press, Oxford, UKGoogle Scholar