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Optical Microscopy of Fluctuating Giant Vesicles and Motile Cells

  • H. -G. Döbereiner
Reference work entry

1 Introduction

1.1 Overview

During the last two decades, optical microscopy has experienced a renaissance. Initiated by the advent of confocal microscopes [1] and optical tweezers [2] in the mid 1980s, as well as 2-photon microscopy [3] in the early 1990s, direct 3-dimensional observation and manipulation of biological material with high resolution continue to deliver many important insights. A more specialized quasi 2-dimensional technique, total internal reflection fluorescence (TIRF) [4] allows observation near a substrate with a comparatively simple set up but superb signal-to-noise ratio. At the same time, ever increasing computer power made it possible to perform advanced real-time image analysis on micrographs obtained with classical phase and differential interference contrast (DIC) [5, 6, 7]. Automated tracking of single particles and membrane movements can be done with nanometer resolution.

This Chapter covers some specific aspects of light microscopy of giant vesicles and...

Keywords

Myosin Light Chain Kinase Bilayer Thickness Total Internal Reflection Fluorescence Spontaneous Curvature Total Internal Reflection Fluorescence Microscopy 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

First and foremost, I would like to thank my former students Astrid Gau, Christopher K. Haluska, Josephine B. Lee, Antje Reinecke, Olaf Selchow, Liyu Xu, as well as my former postdocs Rumiana Dimova, Peter G. Petrov, and Karin Riske for the productive atmosphere in my old group in Postdam. Special thanks to Gerhard Gompper and Udo Seifert for their inspiration and truly enjoyable collaboration. I have benefited greatly from discussions with my collaborators at Columbia University Benjamin J. Dubin-Thaler, Gregory Giannone, and Harry S. Xenias.

I am indebted to my academic teachers Michael Wortis, Evan Evans, Erich Sackmann, Reinhard Lipowsky, and Michael P. Sheetz for their guidance and insight. Special thanks to Michael P. Sheetz for his hospitality and support which allowed this review to be written.

Last but not least, I am grateful for discussions with my colleagues and collaborators Patricia Bassereau, Pieter R. Cullis, Willi Fenzl, Stefan Förster, Werner Goedel, Woijciek T. Góźdź, Christine Hiergeist, Josef Käs, Martin Kraus, Thomas D. Madden, Ling Miao, Barbara L.S. Mui, Jacques Pécréaux, Jacques Prost, and Wolfgang Wintz.

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Authors and Affiliations

  • H. -G. Döbereiner
    • 1
  1. 1.Columbia University New YorkNYUSA

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