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Structural Studies of Proteins and Nucleic Acids in Solution Using Small Angle X-Ray Scattering (SAXS)

  • R. Das
  • S. Doniach
Reference work entry

1 Introduction

Over the last 20 years, the application of small angle x-ray scattering (SAXS) to the structural investigation of biological macromolecules has made tremendous progress. Historically used to measure molecule size, SAXS has evolved into a versatile technique for quantitatively characterizing the features and forces that govern the behavior of biomolecules in solution. This progress has been driven by the dramatically improving brightness of synchrotron radiation x-ray sources combined with the wide availability of new computational resources for interpreting SAXS measurements. This chapter uses contemporary examples to illustrate how solution SAXS provides insights into the chemical physics of proteins and nucleic acids that are difficult – in some cases, impossible – to obtain by other techniques.

The presented examples are ordered from the most straightforward analyses and applications of SAXS experiments to the most sophisticated, and include practical notes on each...

Keywords

Virial Coefficient Conformational Ensemble Guanidinium Hydrochloride Interparticle Interference Biomolecule Concentration 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • R. Das
    • 1
  • S. Doniach
    • 2
  1. 1.Stanford UniversityCAUSA
  2. 2.Stanford UniversityCAUSA

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