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Genomics of Probiotic Bacteria

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Prebiotics and Probiotics Science and Technology

Abstract

Probiotic bacteria from the Lactobacillus and Bifidobacterium species belong to the Firmicutes and the Actinobacteria phylum, respectively. Lactobacilli are members of the lactic acid bacteria (LAB) group, a broadly defined family of microorganisms that ferment various hexoses into primarily lactic acid. Lactobacilli are typically low G + C gram-positive species which are phylogenetically diverse, with over 100 species documented to date. Bifidobacteria are heterofermentative, high G + C content bacteria with about 30 species of bifidobacteria described to date.

*Corresponding author.

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Abbreviations

2CRS :

two-component regulatory system

ABC :

ATP-dependent binding cassettes

BSH :

bile salt hydrolases

COGs :

Clusters of Orthologous Groups of proteins

EMP :

Embden-Meyerhof Pathway

EPS :

exopolysaccaride

Fbp :

fibronectin-binding protein

FOS :

fructo-oligosaccaride

GIT :

gastrointestinal tract

GOS :

galacto-oligosaccaride

HGT :

horizontal gene transfer

IVET :

in vivo expression technology

LAB :

lactic acid bacteria

LaCOGs :

Lactobacillales-specific clusters of orthologous protein coding genes

LTA :

lipotechoic acids

PEP-PTSs :

phosphoenolpyruvate-dependant phosphotransferase system

PKP :

phosphoketolase pathway

SLPs :

surface layer proteins

TA :

techoic acids

References

  • Allison GE, Fremaux C, Klaenhammer TR (1994) Expansion of bacteriocin activity and host range upon complementation of two peptides encoded within the lactacin F operon. J Bacteriol 176:2235–2241

    CAS  Google Scholar 

  • Altermann E, Russell WM, Azcarate-Peril MA, Barrangou R, Buck BL, McAuliffe O, Souther N, Dobson A, Duong T, Callanan M, Lick S, Hamrick A, Cano R, Klaenhammer TR (2005) Complete genome sequence of the probiotic lactic acid bacterium Lactobacillus acidophilus NCFM. Proc Natl Acad Sci USA 102:3906–3912

    Article  CAS  Google Scholar 

  • Azcarate-Peril MA, Altermann E, Goh YJ, Tallon R, Sanozky-Dawes RB, Pfeiler EA, O'Flaherty S, Buck BL, Dobson A, Duong T, Miller MJ, Barrangou R, Klaenhammer TR (2008) Analysis of the genome sequence of Lactobacillus gasseri ATCC 33323 reveals the molecular basis of an autochthonous intestinal organism. Appl Environ Microbiol 74:4610–4625

    Article  CAS  Google Scholar 

  • Azcarate-Peril MA, Altermann E, Hoover-Fitzula RL, Cano RJ, Klaenhammer TR (2004) Identification and inactivation of genetic loci involved with Lactobacillus acidophilus acid tolerance. Appl Environ Microbiol 70:5315–5322

    Article  CAS  Google Scholar 

  • Barrangou R, Altermann E, Hutkins R, Cano R, Klaenhammer TR (2003) Functional and comparative analyses of an operon involved in fructooligosaccharide utilization by Lactobacillus acidophilus. Proc Natl Acad Sci USA 100:8957–8962

    Article  CAS  Google Scholar 

  • Barrangou R, Azcarate-Peril MA, Duong T, Conners SB, Kelly RM, Klaenhammer TR (2006) Global analysis of carbohydrate utilization by Lactobacillus acidophilus using cDNA microarrays. Proc Natl Acad Sci USA 103:3816–3821

    Article  CAS  Google Scholar 

  • Begley M, Hill C, Gahan CG (2006) Bile salt hydrolase activity in probiotics. Appl Environ Microbiol 72:1729–1738

    Article  CAS  Google Scholar 

  • Berger B, Pridmore RD, Barretto C, Delmas-Julien F, Schreiber K, Arigoni F, Brussow H (2007) Similarity and differences in the Lactobacillus acidophilus group identified by polyphasic analysis and comparative genomics. J Bacteriol 189:1311–1321

    Article  CAS  Google Scholar 

  • Bergonzelli GE, Granato D, Pridmore RD, Marvin-Guy LF, Donnicola D, Corthesy-Theulaz IE (2006) GroEL of Lactobacillus johnsonii La1 (NCC 533) is cell surface associated: potential role in interactions with the host and the gastric pathogen Helicobacter pylori. Infect Immun 74:425–434

    Article  CAS  Google Scholar 

  • Blum S, Reniero R, Schiffrin EJ, Crittenden R, Mattila-Sandholm T, Ouwehand AC, Salminen S, von Wright A, Saarela M, Saxelin M, Collins K, Morelli L (2000) Adhesion studies for probiotics: need for validation and refinement. Trends Food Sci Technol 10:405–410

    Article  Google Scholar 

  • Boekhorst J, Helmer Q, Kleerebezem M, Siezen RJ (2006a) Comparative analysis of proteins with a mucus-binding domain found exclusively in lactic acid bacteria. Microbiology 152:273–280

    Article  CAS  Google Scholar 

  • Boekhorst J, Wels M, Kleerebezem M, Siezen RJ (2006b) The predicted secretome of Lactobacillus plantarum WCFS1 sheds light on interactions with its environment. Microbiology 152:3175–3183

    Article  CAS  Google Scholar 

  • Bron PA, Grangette C, Mercenier A, de Vos WM, Kleerebezem M (2004a) Identification of Lactobacillus plantarum genes that are induced in the gastrointestinal tract of mice. J Bacteriol 186:5721–5729

    Article  CAS  Google Scholar 

  • Bron PA, Marco M, Hoffer SM, Van Mullekom E, de Vos WM, Kleerebezem M (2004b) Genetic characterization of the bile salt response in Lactobacillus plantarum and analysis of responsive promoters in vitro and in situ in the gastrointestinal tract. J Bacteriol 186:7829–7835

    Article  CAS  Google Scholar 

  • Bron PA, Molenaar D, de Vos WM, Kleerebezem M (2006) DNA micro-array-based identification of bile-responsive genes in Lactobacillus plantarum. J Appl Microbiol 100:728–738

    Article  CAS  Google Scholar 

  • Buck BL, Altermann E, Svingerud T, Klaenhammer TR (2005) Functional analysis of putative adhesion factors in Lactobacillus acidophilus NCFM. Appl Environ Microbiol 71:8344–8351

    Article  CAS  Google Scholar 

  • Callanan M, Kaleta P, O'Callaghan J, O'Sullivan O, Jordan K, McAuliffe O, Sangrador-Vegas A, Slattery L, Fitzgerald GF, Beresford T, Ross RP (2008) Genome sequence of Lactobacillus helveticus, an organism distinguished by selective gene loss and insertion sequence element expansion. J Bacteriol 190:727–735

    Article  CAS  Google Scholar 

  • Chaillou S, Champomier-Verges MC, Cornet M, Crutz-Le Coq AM, Dudez AM, Martin V, Beaufils S, Darbon-Rongere E, Bossy R, Loux V, Zagorec M (2005) The complete genome sequence of the meat-borne lactic acid bacterium Lactobacillus sakei 23K. Nat Biotechnol 23:1527–3153

    Article  CAS  Google Scholar 

  • Cheikhyoussef A, Pogori N, Chen W, Zhang H (2008) Antimicrobial proteinaceous compounds obtained from bifidobacteria: From production to their application. Int J Food Microbiol 125:215–222

    Article  CAS  Google Scholar 

  • Claesson MJ, Li Y, Leahy S, Canchaya C, van Pijkeren JP, Cerdeno-Tarraga AM, Parkhill J, Flynn S, O'Sullivan GC, Collins JK, Higgins D, Shanahan F, Fitzgerald GF, van Sinderen D, O'Toole PW (2006) Multireplicon genome architecture of Lactobacillus salivarius. Proc Natl Acad Sci USA 103:6718–6723

    Article  CAS  Google Scholar 

  • Collado MC, Sanz Y (2007) Induction of acid resistance in Bifidobacterium: a mechanism for improving desirable traits of potentially probiotic strains. J Appl Microbiol 103:1147–1157

    Article  CAS  Google Scholar 

  • Coor SC, Li Y, Riedel CU, O'Toole PW, Hill CH, Gahan CGM (2007)  Bacteriocin production as a mechanism for the antiinfective activity of Lactobacillus salivarius UCC118. Proc Natl Acad Sci USA 104:7617–7621

    Article  Google Scholar 

  • De Dea Lindner J, Canchaya C, Zhang Z, Neviani E, Fitzgerald GF, van Sinderen D, Ventura M (2007) Exploiting Bifidobacterium genomes: the molecular basis of stress response. Int J Food Microbiol 120:13–24

    Article  CAS  Google Scholar 

  • de Leeuw E, Li X, Lu W (2006) Binding characteristics of the Lactobacillus brevis ATCC 8287 surface layer to extracellular matrix proteins FEMS. Microbiol Lett 260:210–215

    Article  Google Scholar 

  • Delcenserie V, Lessard MH, LaPointe G, Roy D (2008) Genome comparison of Bifidobacterium longum strains NCC2705 and CRC-002 using suppression subtractive hybridization FEMS. Microbiol Lett 280:50–56

    Article  CAS  Google Scholar 

  • Denou E, Berger B, Barretto C, Panoff JM, Arigoni F, Brussow H (2007) Gene expression of commensal Lactobacillus johnsonii strain NCC533 during in vitro growth and in the murine gut. J Bacteriol 189:8109–8119

    Article  CAS  Google Scholar 

  • Denou E, Pridmore RD, Berger B, Panoff JM, Arigoni F, Brussow H (2008) Identification of genes associated with the long-gut-persistence phenotype of the probiotic Lactobacillus johnsonii strain NCC533 using a combination of genomics and transcriptome analysis. J Bacteriol 190:3161–3168

    Article  CAS  Google Scholar 

  • Dobson AE, Sanozky-Dawes RB, Klaenhammer TR (2007) Identification of an operon and inducing peptide involved in the production of lactacin B by Lactobacillus acidophilus. J Appl Microbiol 103:1766–1778

    Article  CAS  Google Scholar 

  • Duong T, Barrangou R, Russell WM, Klaenhammer TR (2006) Characterization of the tre locus and analysis of trehalose cryoprotection in Lactobacillus acidophilus NCFM. Appl Environ Microbiol 72:1218–1225

    Article  CAS  Google Scholar 

  • Elkins CA, Moser SA, Savage DC (2001) Genes encoding bile salt hydrolases and conjugated bile salt transporters in Lactobacillus johnsonii 100-100 and other Lactobacillus species. Microbiology 147:3403–3412

    CAS  Google Scholar 

  • Flynn S, van Sinderen D, Thornton GM, Holo H, Nes IF, Collins JK (2002) Characterization of the genetic locus responsible for the production of ABP-118, a novel bacteriocin produced by the probiotic bacterium Lactobacillus salivarius subsp. salivarius UCC118. Microbiology 148:973–984

    CAS  Google Scholar 

  • Goh YJ, Klaenhammer TR (2008) Genomic features of Lactobacillus species. Front Biosci 14:1362–1386

    Google Scholar 

  • Goh YJ, Zhang C, Benson AK, Schlegel V, Lee JH, Hutkins RW (2006) Identification of a putative operon involved in fructooligosaccharide utilization by Lactobacillus paracasei. Appl Environ Microbiol 72:7518–7530

    Article  CAS  Google Scholar 

  • Granato D, Bergonzelli GE, Pridmore RD, Marvin L, Rouvet M, Corthesy-Theulaz IE (2004) Cell surface-associated elongation factor Tu mediates the attachment of Lactobacillus johnsonii NCC533 (La1) to human intestinal cells and mucins. Infect Immun 72:2160–2169

    Article  CAS  Google Scholar 

  • Granato D, Perotti F, Masserey I, Rouvet M, Golliard M, Servin A, Brassart D (1999) Cell surface-associated lipoteichoic acid acts as an adhesion factor for attachment of Lactobacillus johnsonii La1 to human enterocyte-like Caco-2 cells. Appl Environ Microbiol 65:1071–1077

    CAS  Google Scholar 

  • Grangette C, Nutten S, Palumbo E, Morath S, Hermann C, Dewulf J, Pot B, Hartung T, Hols P, Mercenier A (2005) Enhanced antiinflammatory capacity of a Lactobacillus plantarum mutant synthesizing modified teichoic acids. Proc Natl Acad Sci USA 102:10321–10326

    Article  CAS  Google Scholar 

  • Hartford O, Francois P, Vaudaux P, Foster TJ (1997) The dipeptide repeat region of the fibrinogen-binding protein (clumping factor) is required for functional expression of the fibrinogen-binding domain on the Staphylococcus aureus cell surface. Mol Microbiol 25:1065–1076

    Article  CAS  Google Scholar 

  • Ivanov D, Emonet C, Foata F, Affolter M, Delley M, Fisseha M, Blum-Sperisen S, Kochhar S, Arigoni F (2006) A serpin from the gut bacterium Bifidobacterium longum inhibits eukaryotic elastase-like serine proteases. J Biol Chem 281:17246–17252

    Article  CAS  Google Scholar 

  • Klaenhammer TR (1993) Genetics of bacteriocins produced by lactic acid bacteria FEMS. Microbiol Rev 12:39–85

    CAS  Google Scholar 

  • Klaenhammer TR, Barrangou R, Buck BL, Azcarate-Peril MA, Altermann E (2005) Genomic features of lactic acid bacteria effecting bioprocessing and health FEMS. Microbiol Rev 29:393–409

    CAS  Google Scholar 

  • Kleerebezem M, Boekhorst J, van Kranenburg R, Molenaar D, Kuipers OP, Leer R, Tarchini R, Peters SA, Sandbrink HM, Fiers MW, Stiekema W, Lankhorst RM, Bron PA, Hoffer SM, Groot MN, Kerkhoven R, de Vries M, Ursing B, de Vos WM, Siezen RJ (2003) Complete genome sequence of Lactobacillus plantarum WCFS1. Proc Natl Acad Sci USA 100:1990–1995

    Article  CAS  Google Scholar 

  • Klijn A, Mercenier A, Arigoni F (2005) Lessons from the genomes of bifidobacteria FEMS. Microbiol Rev 29:491–509

    CAS  Google Scholar 

  • Kullen MJ, Klaenhammer TR (1999) Identification of the pH-inducible, proton-translocating F1F0-ATPase (atpBEFHAGDC) operon of Lactobacillus acidophilus by differential display: gene structure, cloning and characterization. Mol Microbiol 33:1152–1161

    Article  CAS  Google Scholar 

  • Lambert JM, Bongers RS, de Vos WM, Kleerebezem M (2008) Functional analysis of four bile salt hydrolase and penicillin acylase family members in Lactobacillus plantarum WCFS1. Appl Environ Microbiol 74:4719–4726

    Article  CAS  Google Scholar 

  • Lee JH, Karamychev VN, Kozyavkin SA, Mills D, Pavlov AR, Pavlova NV, Polouchine NN, Richardson PM, Shakhova VV, Slesarev AI, Weimer B, O'Sullivan DJ (2008) Comparative genomic analysis of the gut bacterium Bifidobacterium longum reveals loci susceptible to deletion during pure culture growth BMC. Genomics 9:247

    Article  Google Scholar 

  • Liu M, van Enckevort FH, Siezen RJ (2005) Genome update: lactic acid bacteria genome sequencing is booming. Microbiology 151:3811–3814

    Article  CAS  Google Scholar 

  • MacConaill LE, Fitzgerald GF, Van Sinderen D (2003) Investigation of protein export in Bifidobacterium breve UCC2003. Appl Environ Microbiol 69:6994–7001

    Article  CAS  Google Scholar 

  • Makarova K, Slesarev A, Wolf Y, Sorokin A, Mirkin B, Koonin E, Pavlov A, Pavlova N, Karamychev V, Polouchine N, Shakhova V, Grigoriev I, Lou Y, Rohksar D, Lucas S, Huang K, Goodstein DM, Hawkins T, Plengvidhya V, Welker D, Hughes J, Goh Y, Benson A, Baldwin K, Lee JH, Diaz-Muniz I, Dosti B, Smeianov V, Wechter W, Barabote R, Lorca G, Altermann E, Barrangou R, Ganesan B, Xie Y, Rawsthorne H, Tamir D, Parker C, Breidt F, Broadbent J, Hutkins R, O'Sullivan D, Steele J, Unlu G, Saier M, Klaenhammer T, Richardson P, Kozyavkin S, Weimer B, Mills D (2006) Comparative genomics of the lactic acid bacteria. Proc Natl Acad Sci USA 103:15611–15616

    Article  Google Scholar 

  • Makarova KS, Koonin EV (2007) Evolutionary genomics of lactic acid bacteria. J Bacteriol 189:1199–1208

    Article  CAS  Google Scholar 

  • Makino S, Ikegami S, Kano H, Sashihara T, Sugano H, Horiuchi H, Saito T, Oda M (2006) Immunomodulatory effects of polysaccharides produced by Lactobacillus delbrueckii ssp. bulgaricus OLL1073R-1. J Dairy Sci 89:2873–2881

    Article  CAS  Google Scholar 

  • McAuliffe O, Cano RJ, Klaenhammer TR (2005) Genetic analysis of two bile salt hydrolase activities in Lactobacillus acidophilus NCFM. Appl Environ Microbiol 71:4925–4929

    Article  CAS  Google Scholar 

  • Morita H, Toh H, Fukuda S, Horikawa H, Oshima K, Suzuki T, Murakami M, Hisamatsu S, Kato Y, Takizawa T, Fukuoka H, Yoshimura T, Itoh K, O'Sullivan DJ, McKay LL, Ohno H, Kikuchi J, Masaoka T, Hattori M (2008) Comparative Genome Analysis of Lactobacillus reuteri and Lactobacillus fermentum Reveal a Genomic Island for Reuterin and Cobalamin Production DNA. Res 15:151–161

    CAS  Google Scholar 

  • Palmer C, Bik EM, Digiulio DB, Relman DA, Brown PO (2007) Development of the Human Infant Intestinal Microbiota PLoS. Biol 5:e177

    Google Scholar 

  • Pfeiler EA, Azcarate-Peril MA, Klaenhammer TR (2007) Characterization of a novel bile-inducible operon encoding a two-component regulatory system in Lactobacillus acidophilus. J Bacteriol 189:4624–4634

    Article  CAS  Google Scholar 

  • Pieterse B, Leer RJ, Schuren FH, van der Werf MJ (2005) Unravelling the multiple effects of lactic acid stress on Lactobacillus plantarum by transcription profiling. Microbiology 151:3881–3894

    Article  CAS  Google Scholar 

  • Pretzer G, Snel J, Molenaar D, Wiersma A, Bron PA, Lambert J, de Vos WM, van der Meer R, Smits MA, Kleerebezem M (2005) Biodiversity-based identification and functional characterization of the mannose-specific adhesin of Lactobacillus plantarum. J Bacteriol 187:6128–6136

    Article  CAS  Google Scholar 

  • Pridmore RD, Berger B, Desiere F, Vilanova D, Barretto C, Pittet AC, Zwahlen MC, Rouvet M, Altermann E, Barrangou R, Mollet B, Mercenier A, Klaenhammer T, Arigoni F, Schell MA (2004) The genome sequence of the probiotic intestinal bacterium Lactobacillus johnsonii NCC 533. Proc Natl Acad Sci USA 101:2512–2517

    Article  CAS  Google Scholar 

  • Reid G, Sanders ME, Gaskins HR, Gibson GR, Mercenier A, Rastall R, Roberfroid M, Rowland I, Cherbut C, Klaenhammer TR (2003) New scientific paradigms for probiotics and prebiotics. J Clin Gastroenterol 37:105–118

    Article  Google Scholar 

  • Roos S, Jonsson H (2002) A high-molecular-mass cell-surface protein from Lactobacillus reuteri 1063 adheres to mucus components. Microbiology 148:433–442

    CAS  Google Scholar 

  • Ryan SM, Fitzgerald GF, van Sinderen D (2005) Transcriptional regulation and characterization of a novel beta-fructofuranosidase-encoding gene from Bifidobacterium breve UCC2003. Appl Environ Microbiol 71:3475–3482

    Article  CAS  Google Scholar 

  • Ryan SM, Fitzgerald GF, van Sinderen D (2006) Screening for and identification of starch-, amylopectin-, and pullulan-degrading activities in bifidobacterial strains. Appl Environ Microbiol 72:5289–5296

    Article  CAS  Google Scholar 

  • Salazar N, Gueimonde M, Hernandez-Barranco AM, Ruas-Madiedo P, de los Reyes-Gavilan CG (2008) Exopolysaccharides produced by intestinal Bifidobacterium strains act as fermentable substrates for human intestinal bacteria. Appl Environ Microbiol 74:4737–4745

    Article  CAS  Google Scholar 

  • Sanchez B, Champomier-Verges MC, Collado Mdel C, Anglade P, Baraige F, Sanz Y, de los Reyes-Gavilan CG, Margolles A, Zagorec M (2007) Low-pH adaptation and the acid tolerance response of Bifidobacterium longum biotype longum. Appl Environ Microbiol 73:6450–6459

    Article  CAS  Google Scholar 

  • Saulnier DM, Molenaar D, de Vos WM, Gibson GR, Kolida S (2007) Identification of prebiotic fructooligosaccharide metabolism in Lactobacillus plantarum WCFS1 through microarrays. Appl Environ Microbiol 73:1753–1765

    Article  CAS  Google Scholar 

  • Schell MA, Karmirantzou M, Snel B, Vilanova D, Berger B, Pessi G, Zwahlen MC, Desiere F, Bork P, Delley M, Pridmore RD, Arigoni F (2002) The genome sequence of Bifidobacterium longum reflects its adaptation to the human gastrointestinal tract. Proc Natl Acad Sci USA 99:14422–14427

    Article  CAS  Google Scholar 

  • Serrano LM, Molenaar D, Wels M, Teusink B, Bron PA, de Vos WM, Smid EJ (2007) Thioredoxin reductase is a key factor in the oxidative stress response of Lactobacillus plantarum WCFS1. Microb Cell Fact 6:29

    Article  Google Scholar 

  • Sonnenburg JL, Chen CT, Gordon JI (2006) Genomic and metabolic studies of the impact of probiotics on a model gut symbiont and host PLoS. Biol 4:e413

    Google Scholar 

  • Sturme MH, Nakayama J, Molenaar D, Murakami Y, Kunugi R, Fujii T, Vaughan EE, Kleerebezem M, de Vos WM (2005) An agr-like two-component regulatory system in Lactobacillus plantarum is involved in production of a novel cyclic peptide and regulation of adherence. J Bacteriol 187:5224–5235

    Article  CAS  Google Scholar 

  • van de Guchte M, Penaud S, Grimaldi C, Barbe V, Bryson K, Nicolas P, Robert C, Oztas S, Mangenot S, Couloux A, Loux V, Dervyn R, Bossy R, Bolotin A, Batto JM, Walunas T, Gibrat JF, Bessieres P, Weissenbach J, Ehrlich SD, Maguin E (2006) The complete genome sequence of Lactobacillus bulgaricus reveals extensive and ongoing reductive evolution. Proc Natl Acad Sci USA 103:9274–9279

    Article  Google Scholar 

  • van Pijkeren JP, Canchaya C, Ryan KA, Li Y, Claesson MJ, Sheil B, Steidler L, O'Mahony L, Fitzgerald GF, van Sinderen D, O'Toole PW (2006) Comparative and functional analysis of sortase-dependent proteins in the predicted secretome of Lactobacillus salivarius UCC118. Appl Environ Microbiol 72:4143–4153

    Article  Google Scholar 

  • Ventura M, Canchaya C, Kleerebezem M, de Vos WM, Siezen RJ, Brussow H (2003) The prophage sequences of Lactobacillus plantarum strain WCFS1. Virology 316:245–255

    Article  CAS  Google Scholar 

  • Ventura M, Lee JH, Canchaya C, Zink R, Leahy S, Moreno-Munoz JA, O'Connell-Motherway M, Higgins D, Fitzgerald GF, O'Sullivan DJ, van Sinderen D (2005) Prophage-like elements in bifidobacteria: insights from genomics, transcription, integration, distribution, and phylogenetic analysis. Appl Environ Microbiol 71:8692–8705

    Article  CAS  Google Scholar 

  • Ventura M, O'Connell-Motherway M, Leahy S, Moreno-Munoz JA, Fitzgerald GF, van Sinderen D (2007) From bacterial genome to functionality; case bifidobacteria. Int J Food Microbiol 120:2–12

    Article  CAS  Google Scholar 

  • Vernazza CL, Gibson GR, Rastall RA (2006) Carbohydrate preference, acid tolerance and bile tolerance in five strains of Bifidobacterium. J Appl Microbiol 100:846–853

    Article  CAS  Google Scholar 

  • Wall T, Bath K, Britton RA, Jonsson H, Versalovic J, Roos S (2007) The early response to acid shock in Lactobacillus reuteri involves the ClpL chaperone and a putative cell wall-altering esterase. Appl Environ Microbiol 73:3924–3935

    Article  CAS  Google Scholar 

  • Walter J, Chagnaud P, Tannock GW, Loach DM, Dal Bello F, Jenkinson HF, Hammes WP, Hertel C (2005) A high-molecular-mass surface protein (Lsp) and methionine sulfoxide reductase B (MsrB) contribute to the ecological performance of Lactobacillus reuteri in the murine gut. Appl Environ Microbiol 71:979–986

    Article  CAS  Google Scholar 

  • Walter J, Heng NC, Hammes WP, Loach DM, Tannock GW, Hertel C (2003) Identification of Lactobacillus reuteri genes specifically induced in the mouse gastrointestinal tract. Appl Environ Microbiol 69:2044–2051

    Article  CAS  Google Scholar 

  • Whitehead K, Versalovic J, Roos S, Britton RA (2008) Genomic and genetic characterization of the bile stress response of probiotic Lactobacillus reuteri ATCC 55730. Appl Environ Microbiol 74:1812–1819

    Article  CAS  Google Scholar 

  • Whitman WB, Coleman DC, Wiebe WJ (1998) Prokaryotes: the unseen majority. Proc Natl Acad Sci USA 95:6578–6583

    Article  CAS  Google Scholar 

  • Yasuda E, Serata M, Sako T (2008) Suppressive effect on activation of macrophages by Lactobacillus casei strain Shirota genes determining the synthesis of cell wall-associated polysaccharides. Appl Environ Microbiol 74:4746–4755

    Article  CAS  Google Scholar 

  • Yuan J, Wang B, Sun Z, Bo X, Yuan X, He X, Zhao H, Du X, Wang F, Jiang Z, Zhang L, Jia L, Wang Y, Wei K, Wang J, Zhang X, Sun Y, Huang L, Zeng M (2008) Analysis of host-inducing proteome changes in Bifidobacterium longum NCC2705 grown in Vivo. J Proteome Res 7:375–385

    Article  CAS  Google Scholar 

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Acknowledgment

The research program on probiotic lactobacilli at North Carolina State University is supported by the North Carolina Dairy Foundation, Danisco USA, Inc., Dairy Management Inc., and the Southeast Dairy Foods Research Center.

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Authors and Affiliations

  1. Department of Food, Bioprocessing, and Nutrition Sciences; and Southeast Dairy Foods Research Center, North Carolina State University, Raleigh, NC, USA

    Sarah O’Flaherty, Yong Jun Goh & Todd R. Klaenhammer

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  1. Sarah O’Flaherty
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  2. Yong Jun Goh
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  3. Todd R. Klaenhammer
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  1. Department of Food Biosciences, University of Reading Whiteknights, Reading, UK

    Dimitris Charalampopoulos  & Robert A. Rastall  & 

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O’Flaherty, S., Goh, Y.J., Klaenhammer, T.R. (2009). Genomics of Probiotic Bacteria. In: Charalampopoulos, D., Rastall, R.A. (eds) Prebiotics and Probiotics Science and Technology. Springer, New York, NY. https://doi.org/10.1007/978-0-387-79058-9_17

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