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Principles and Applications of Proteomics in Pancreatic Cancer

  • Sarah Tonack
  • John Neoptolemos
  • Eithne Costello
Reference work entry

Abstract:

The proteome is the complete set of proteins expressed in a subcellular or cellular compartment, tissue, biological fluid, or organism. It is estimated that there are some 250,000–300,000 human proteins, encoded from 20,000 to 25,000 genes. The discrepancy between the number of genes and the number of proteins is due in part to alternative gene splicing and post-translational modification of proteins.

The proteome is dynamic and changes in response to both intracellular and extracellular signaling. Therefore, comparisons of protein profiles from normal and malignant pancreatic cells, or cells from distinct histological stages that occur during the development of pancreatic cancer provide opportunities for further understanding the pathogenesis of this tumor type. Moreover, such analyses as well as detailed proteomic studies of body fluids, such as pancreatic juice, serum, plasma or urine will likely facilitate the elucidation of new biomarkers for the diagnosis, or management of this disease.

Although proteomic technologies have been rapidly advancing for a number of years, the last decade in particular has seen developments that enable the simultaneous study of large numbers of proteins in a single experiment or set of experiments. In this chapter, the proteomic-based approaches that are most commonly used to analyze pancreatic cancer specimens are reviewed. The range of sample types that have been subjected to analysis, including samples from animal models of pancreatic cancer are discussed, along with examples of proteins that have been identified in these studies.

Keywords

Pancreatic Cancer Chronic Pancreatitis Pancreatic Juice Pancreatic Cancer Cell Line Pancreatic Cancer Patient 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Sarah Tonack
    • 1
  • John Neoptolemos
    • 1
  • Eithne Costello
    • 1
  1. 1.Division of Surgery and Oncology, Royal Liverpool University HospitalUniversity of Liverpool UK

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