Encyclopedia of Biophysics

Living Edition
| Editors: Gordon Roberts, Anthony Watts, European Biophysical Societies

Oriented Circular Dichroism Spectroscopy

  • Alison Rodger
Living reference work entry
DOI: https://doi.org/10.1007/978-3-642-35943-9_639-1

Synonyms

OCD

Definition

Oriented circular dichroism (OCD) spectroscopy, as the name implies, is the circular dichroism spectroscopy of oriented samples. It can be a very useful technique; however, it is prone to artifacts resulting from linear dichroism contributions to the observed spectrum (Nordén et al. 2010). Thus care must be taken in collecting and analyzing the data. A sample for OCD ideally should be symmetric about a unique orientation axis. Data must then be collected with the light propagating along that unique axis and spectra measured at different rotations about the unique axis compared. If the rotated spectra are the same, then these data can be believed. If the sample is perfectly oriented (as it would be in a perfectly oriented crystal but probably in no other method), the observed spectrum is then equivalent to three times (no rotational averaging) the CD spectrum of all the transitions in an isotropic sample minus those polarized along the unique axis (which are...

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References

  1. Damianoglou A, Rodger A, Pridmore C, Dafforn TR, Mosely JA, Sanderson JM, Hicks MR (2010) The synergistic action of melittin and phospholipase A2 with lipid membranes: development of linear dichroism for membrane-insertion kinetics. Protein Pept Lett 17:1351–1362CrossRefPubMedGoogle Scholar
  2. Nordén B, Rodger A, Dafforn TR (2010) Linear dichroism and circular dichroism: a textbook on polarized spectroscopy. Royal Society of Chemistry, CambridgeGoogle Scholar

Copyright information

© European Biophysical Societies' Association (EBSA) 2018

Authors and Affiliations

  1. 1.Department of Molecular SciencesMacquarie UniversityMacquarie ParkAustralia

Section editors and affiliations

  • Alison Rodger
    • 1
  1. 1.Department of Molecular Sciences, Macquarie UniversityNWSAustralia