Electron Microscopy of Membrane Lipids
Electron microscopy is a method for examining materials using an electron microscope. Electron microscopes use electrons instead of light, which requires that the objects under study be placed in a high vacuum.
Membrane lipids are lipid molecules that are major structural components of biological membranes. They include glycerophospholipids, sphingolipids, and cholesterol (or ergosterol in plants).
A lipid bilayer forms the basic structure of most biological membranes. The lipids in the membrane generally consist of phospholipids, sphingolipids, and cholesterol (or ergosterol in plants). The membrane exhibits a two-dimensional fluidity at ambient temperature, meaning that molecules diffuse laterally in the membrane plane. The distance that a molecule can diffuse in a certain time t can be calculated by the equation, d 2 = 4kt (where d is the distance and kis a diffusion coefficient). The diffusion coefficient for...
- Severs NJ, Shotton DM (eds) (1995) Rapid freezing, freeze fracture, and deep etching. Wiley-Liss, New YorkGoogle Scholar