Encyclopedia of Biophysics

Living Edition
| Editors: Gordon Roberts, Anthony Watts, European Biophysical Societies

Transient-State Kinetic Methods

  • Clive R. BagshawEmail author
Living reference work entry

Later version available View entry history

DOI: https://doi.org/10.1007/978-3-642-35943-9_56-1



Transient-state kinetics refers to the time course of a reaction before it reaches a steady-state or equilibrium position. The concentrations of species change in time according to the Law of Mass Action and hence analysis of the progress of a reaction can lead to the determination of the rate constants of the elementary steps that make up the pathway. Such a pathway, in conjunction with structural information, allows the mechanism of the reaction to be deduced (Bagshaw 2017; Fersht 1985; Goody and Hofmann-Goody 2002; Gutfreund 1975, 1995). A multistep reaction pathway is normally investigated by dissecting it into individual resolvable steps. However, coupling between the steps may complicate the overall reaction profile. In practice, the resolvable phases are first analyzed using analytical solutions to simple reaction schemes (Capellos and Bielski 1980). The reaction schemes are then assembled to give the whole pathway,...

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Copyright information

© European Biophysical Societies' Association (EBSA) 2018

Authors and Affiliations

  1. 1.Department of Chemistry and BiochemistryUniversity of California Santa CruzSanta CruzUSA

Section editors and affiliations

  • Clive R. Bagshaw
    • 1
  1. 1.Department of Chemistry and BiochemistryUniversity of California at Santa CruzSanta CruzUSA