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Force-Fluorescence Spectroscopy

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Encyclopedia of Biophysics

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Synonyms

Single-molecule methods

Definition

Force-fluorescence spectroscopy generally refers to the combination of single-molecule manipulation technique with simultaneous single-molecule fluorescence measurement to characterize the mechanochemical properties of a macromolecule.

Basic Characteristics

On the one hand, single-molecule manipulation using scanning probe techniques, optical tweezers, and magnetic tweezers enables mechanical perturbation of a system at the single-molecule level. On the other hand, intermediates in time-dependent reactions that are otherwise difficult to study in conventional ensemble experiments due to the averaging over many molecules can now be observed and studied directly using single-molecule fluorescence techniques. The combination of single-molecule manipulation techniques with single-molecule fluorescence measurements can thus find many potential applications in biophysical research, for example, manipulation of a macromolecule, while observing...

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Author information

Authors and Affiliations

  1. Department of Pharmaceutical Sciences, Department of Biophysics, Department of Biological Chemistry, University of Michigan, Ann Arbor, MI, USA

    Wei Cheng

Authors
  1. Wei Cheng
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Corresponding author

Correspondence to Wei Cheng .

Editor information

Editors and Affiliations

  1. University Leicester MRC Centre, Leicester, United Kingdom

    Gordon Roberts

  2. Dept Biochemistry, University of Oxford, Oxford, United Kingdom

    Anthony Watts

Section Editor information

  1. Department of Chemistry, The University of Michigan, Ann Arbor, MI, USA

    Nils G. Walter

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© 2018 European Biophysical Societies' Association (EBSA)

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Cheng, W. (2018). Force-Fluorescence Spectroscopy. In: Roberts, G., Watts, A. (eds) Encyclopedia of Biophysics. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-35943-9_521-1

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  • DOI: https://doi.org/10.1007/978-3-642-35943-9_521-1

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  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-35943-9

  • Online ISBN: 978-3-642-35943-9

  • eBook Packages: Springer Reference Biomedicine and Life SciencesReference Module Biomedical and Life Sciences

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