Encyclopedia of Biophysics

Living Edition
| Editors: Gordon Roberts, Anthony Watts, European Biophysical Societies

Force-Fluorescence Spectroscopy

  • Wei ChengEmail author
Living reference work entry
DOI: https://doi.org/10.1007/978-3-642-35943-9_521-1



Force-fluorescence spectroscopy generally refers to the combination of single-molecule manipulation technique with simultaneous single-molecule fluorescence measurement to characterize the mechanochemical properties of a macromolecule.

Basic Characteristics

On the one hand, single-molecule manipulation using scanning probe techniques, optical tweezers, and magnetic tweezers enables mechanical perturbation of a system at the single-molecule level. On the other hand, intermediates in time-dependent reactions that are otherwise difficult to study in conventional ensemble experiments due to the averaging over many molecules can now be observed and studied directly using single-molecule fluorescence techniques. The combination of single-molecule manipulation techniques with single-molecule fluorescence measurements can thus find many potential applications in biophysical research, for example, manipulation of a macromolecule, while observing...

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Copyright information

© European Biophysical Societies' Association (EBSA) 2018

Authors and Affiliations

  1. 1.Department of Pharmaceutical Sciences, Department of Biophysics, Department of Biological ChemistryUniversity of MichiganAnn ArborUSA

Section editors and affiliations

  • Nils G. Walter
    • 1
  1. 1.Department of ChemistryThe University of MichiganAnn ArborUSA