Encyclopedia of Biophysics

Living Edition
| Editors: Gordon Roberts, Anthony Watts, European Biophysical Societies

Electrophoretic Light Scattering

  • Jason CW Corbett
  • Fraser McNeil-Watson
Living reference work entry
DOI: https://doi.org/10.1007/978-3-642-35943-9_288-1

Synonyms

Introduction

Electrophoretic light scattering, commonly abbreviated to ELS, is an analytical method that measures the electrophoretic mobility of particles or macromolecules in suspension. As in the related DLS (dynamic light scattering) method, the suspension is illuminated by a narrow light beam, generally a laser beam of a visible wavelength (∼400–800 nm). Under the application of an electric field, electrically charged entities in suspension will undergo directed motion toward their respective counter electrodes, depending on the sign of their overall charge, superimposed upon their randomly directed Brownian motion as measured using DLS. The directed component imparts a Doppler shift on the light scattered by the dispersed entities, from the illuminating light beam, which is collected by the optical system and from which...

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Copyright information

© European Biophysical Societies' Association (EBSA) 2018

Authors and Affiliations

  1. 1.Malvern Instruments LimitedMalvernUK

Section editors and affiliations

  • Stephen E. Harding
    • 1
  • Mary Philips-Jones
  1. 1.School of Biosciences, NCMH LaboratoryUniversity of NottinghamSutton BoningtonUK