Electrophoretic Light Scattering
Electrophoretic light scattering, commonly abbreviated to ELS, is an analytical method that measures the electrophoretic mobility of particles or macromolecules in suspension. As in the related DLS (dynamic light scattering) method, the suspension is illuminated by a narrow light beam, generally a laser beam of a visible wavelength (∼400–800 nm). Under the application of an electric field, electrically charged entities in suspension will undergo directed motion toward their respective counter electrodes, depending on the sign of their overall charge, superimposed upon their randomly directed Brownian motion as measured using DLS. The directed component imparts a Doppler shift on the light scattered by the dispersed entities, from the illuminating light beam, which is collected by the optical system and from which...
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