References
Alley WR Jr, Mann BF, Novotny MV (2013) High-sensitivity analytical approaches for the structural characterization of glycoproteins. Chem Rev 113:2668–2732
Bitto D, Harvey DJ, Halldorsson S et al. (2015) Determination of N-linked glycosylation in UUKV glycoproteins by negative ion mass spectrometry and ion mobility. Methods Mol Biol 1331:93–121
Börnsen KO, Mohr MD, Widmer HM (1995) Ion exchange and purification of carbohydrates on a Nafion(R) membrane as a new sample pretreatment for matrix-assisted laser desorption-ionization mass spectrometry. Rapid Commun Mass Spectrom 9:1031–1034
Carpita NC, Shea EM (1989) Linkage structure of carbohydrates by gas chromatography-mass spectrometry (GC-MS) of partially methylated alditol acetates. In: Biermann CJ, McGinnis GD (eds) Analysis of carbohydrates by GLC and MS. CRC Press, Boca Raton, pp 157–216
Carr SA, Barr JR, Roberts GD et al. (1990) Identification of attachment sites and structural classes of asparagine-linked carbohydrates in glycoproteins. Methods Enzymol 193:501–518
Ceroni A, Maass K, Geyer H et al. (2008) GlycoWorkbench: a tool for the computer-assisted annotation of mass spectra of glycans. J Proteome Res 7:1650–1659
Domon B, Costello CE (1988) A systematic nomenclature for carbohydrate fragmentations in FAB-MS/MS spectra of glycoconjugates. Glycoconj J 5:397–409
Fenn LS, McLean JA (2009) Simultaneous glycoproteomics on the basis of structure using ion mobility-mass spectrometry. Mol BioSyst 5:1298–1302
Greer FM, Morris HR (1997) Fast-atom bombardment and electrospray mass spectrometry of peptides, proteins, and glycoproteins. Methods Mol Biol 64:147–163
Han L, Costello CE (2013) Mass spectrometry of glycans. Biochem Mosc 78:710–720
Harvey DJ (1999) Matrix-assisted laser desorption/ionization mass spectrometry of carbohydrates. Mass Spectrom Rev 18:349–451
Harvey DJ (2009) In-gel enzymatic release of N-glycans. In: The protein protocols handbook, 3rd edn. Humana Press, New York, pp 1357–1364
Harvey DJ (2011) Derivatization of carbohydrates for analysis by chromatography, electrophoresis and mass spectrometry. J Chromatogr B 879:1196–1225
Harvey DJ (2016a) Analysis of protein glycosylation by mass spectrometry. In: Griffiths JR, Unwin RD (eds) Analysis of protein post-translational modifications by mass spectrometry. Wiley, Hoboken, pp 89–159
Harvey DJ (2016b) Carbohydrate analysis by ESI and MALDI. In: Cole RB (ed) Electrospray and MALDI mass spectrometry fundamentals, instrumentation, practicalities and biological applications. Wiley, Hobokken, pp 723–769
Harvey DJ (2018) Analysis of carbohydrates and glycoconjugates by matrix-assisted laser desorption/ionization mass spectrometry: an update for 2013–2014. Mass Spectrom Rev 37:353–491
Harvey DJ, Royle L, Radcliffe CM et al. (2008) Structural and quantitative analysis of N-linked glycans by MALDI and negative ion nanospray mass spectrometry. Anal Biochem 376:44–60
Harvey DJ, Merry AH, Royle L et al. (2009) Proposal for a standard system for drawing structural diagrams of N- and O-linked carbohydrates and related compounds. Proteomics 9:3796–3801
Harvey DJ, Sobott F, Crispin M et al. (2011) Ion mobility mass spectrometry for extracting spectra of N-glycans directly from incubation mixtures following glycan release: application to glycans from engineered glycoforms of intact, folded HIV gp120. J Am Soc Mass Spectrom 22:568–581
Hillenkamp F, Peter-Katalinic J (eds) (2014) MALDI MS: a practical guide to instrumentation, methods and applications. Wiley VCH, Weinheim
Hossain M, Limbach PA (2010) A comparison of MALDI matrices. In: Cole RB (ed) Electrospray and MALDI mass spectrometry: fundamentals, instrumentation, practicalities and biological applications, 2nd edn. Wiley, Hoboken, pp 215–244
Jensen PH, Karlsson NG, Kolarich D et al. (2012) Structural analysis of N- and O-glycans released from glycoproteins. Nat Protoc 7:1299–1300
Küster B, Wheeler SF, Hunter AP, Dwek RA, Harvey DJ (1997) Sequencing of N-linked oligosaccharides directly from protein gels: In-gel deglycosylation followed by matrix-assisted laser desorption/ionization mass spectrometry and normal-phase high performance liquid chromatography. Anal Biochem 250:82–101
Mechref Y, Novotny MV, Krishnan C (2003) Structural characterization of oligosaccharides using MALDI-TOF/TOF tandem mass spectrometry. Anal Chem 75:4895–4903
Novotny MV, Alley WR Jr, Mann BF (2013) Analytical glycobiology at high sensitivity: current approaches and directions. Glycoconj J 30:89–117
Packer NH, Lawson MA, Jardine DR et al. (1998) A general approach to desalting oligosaccharides released from glycoproteins. Glycoconj J 15:737–747
Patel T, Bruce J, Merry A et al. (1993) Use of hydrazine to release in intact and unreduced form both N- and O-linked oligosaccharides from glycoproteins. Biochemistry 32:679–693
Powell AK, Harvey DJ (1996) Stabilisation of sialic acids in N-linked oligosaccharides and gangliosides for analysis by positive ion matrix-assisted laser desorption-ionization mass spectrometry. Rapid Commun Mass Spectrom 10:1027–1032
Reiding KR, Blank D, Kuijper DM et al. (2014) High-throughput profiling of protein N-glycosylation by MALDI-TOF-MS employing linkage-specific sialic acid esterification. Anal Chem 86:5784–5793
Ruhaak LR, Huhn C, Koeleman CAM et al. (2012) Robust and high-throughput sample preparation for (semi-)quantitative analysis of N-glycosylation profiles from plasma samples. Methods Mol Biol 893:371–385
Selman MHJ, Hemayatkar M, Deelder AM et al. (2011) Cotton HILIC SPE microtips for microscale purification and enrichment of glycans and glycopeptides. Anal Chem 83:2492–2499
Stanley P, Taniguchi N, Markus A (2017) Chapter 9, N-Glycans. In: Varki A, Cummings RD, Esko JD et al. (eds) Essentials of glycobiology [Internet], 3rd edn. Cold Spring Harbor Laboratory Press, New York
Stephens E, Maslen SL, Green LG et al. (2004) Fragmentation characteristics of neutral N-linked glycans using a MALDI-TOF/TOF tandem mass spectrometer. Anal Chem 76:2343–2354
Wheeler SF, Domann P, Harvey DJ (2009) Derivatization of sialic acids for stabilization in matrix-assisted laser desorption/ionization mass spectrometry and concomitant differentiation of α(2-3) and α(2-6) isomers. Rapid Commun Mass Spectrom 23:303–312
Woodin CL, Maxon M, Desaire H (2013) Software for automated interpretation of mass spectrometry data from glycans and glycopeptides. Analyst 138:2793–2803
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Section Editor information
Rights and permissions
Copyright information
© 2018 European Biophysical Societies' Association (EBSA)
About this entry
Cite this entry
Harvey, D.J. (2018). Mass Spectrometry of N-Linked Carbohydrates and Glycoproteins. In: Roberts, G., Watts, A. (eds) Encyclopedia of Biophysics. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-35943-9_217-1
Download citation
DOI: https://doi.org/10.1007/978-3-642-35943-9_217-1
Received:
Accepted:
Published:
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-35943-9
Online ISBN: 978-3-642-35943-9
eBook Packages: Springer Reference Biomedicine and Life SciencesReference Module Biomedical and Life Sciences