Encyclopedia of Biophysics

Living Edition
| Editors: Gordon Roberts, Anthony Watts, European Biophysical Societies

Mass Spectrometry of N-Linked Carbohydrates and Glycoproteins

  • David John Harvey
Living reference work entry
DOI: https://doi.org/10.1007/978-3-642-35943-9_217-1

Introduction

N-linked glycans are those attached to proteins with an amide bond to asparagine in an Asp-Xxx-Ser (or -Thr or, occasionally, -Cys) motif where Xxx is any amino acid except proline. Their structures are generally well defined (Stanley et al. 2017), unlike the O-linked glycans (those attached directly to serine or threonine) which, although usually smaller, have more diverse structures and are, consequently, more difficult to analyze. Typical structures of N-glycans are shown in Fig. 1. All have a common trimannosyl chitobiose (Man 3GlcNAc 2) core with several attached antennae, and their analysis, therefore, requires the identification of factors such as the type of glycan (high-mannose, hybrid, or complex (see Fig. 1)), the number and composition of each antenna, number and location of fucose residues, presence or absence of a bisecting GlcNAc residue (β1 → 4-linked to the core mannose residue), and the presence of any further substitution, particularly sialylation....
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References

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Copyright information

© European Biophysical Societies' Association (EBSA) 2018

Authors and Affiliations

  1. 1.Target Discovery Institute, Nuffield Department of MedicineUniversity of OxfordOxfordUK

Section editors and affiliations

  • Neil J. Oldham
    • 1
  1. 1.School of ChemistryThe University of NottinghamNottinghamUK