Encyclopedia of Biophysics

Living Edition
| Editors: Gordon Roberts, Anthony Watts, European Biophysical Societies

CEST and DEST Experiments

  • Shahid N. KhanEmail author
  • John D. Persons
  • Rieko Ishima
Living reference work entry
DOI: https://doi.org/10.1007/978-3-642-35943-9_10073-1


Many important biological processes, such as protein folding, misfolding, ligand binding, enzyme catalysis, and molecular recognition, are often better explained, not only by assuming the single highly populated conformation (major state) but also by assuming conformers populated at very low levels (minor states). Some of such minor states are not detected in traditional NMR spectra because of their low populations but are detectable through the exchange process with the major state by NMR. Compared to other spectroscopic techniques, such as circular dichroism and fluorescence spectroscopy that provide only population weighted-average signals, the detection of minor species through observation of a signal from the major species is a unique advantage of NMR. Recent developments in NMR methodology have provided extensive information about such “invisible” minor conformers that exchange with major species over a wide range of timescale. These experiment include measurement of...

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Copyright information

© European Biophysical Societies’ Association (EBSA) 2018

Authors and Affiliations

  • Shahid N. Khan
    • 1
    Email author
  • John D. Persons
    • 1
  • Rieko Ishima
    • 1
  1. 1.Department of Structural BiologyUniversity of Pittsburgh, School of MedicinePittsburghUSA

Section editors and affiliations

  • Mitsu Ikura

There are no affiliations available